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Integrated research on food chemistry, clinical medicine, and breeding for allergen-free and allergen reduced buckwheat

Research Project

Project/Area Number 16H02530
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Science in genetics and breeding
Research InstitutionUniversity of Tsukuba

Principal Investigator

Ohsawa Ryo  筑波大学, 生命環境系, 教授 (80211788)

Co-Investigator(Kenkyū-buntansha) 佐藤 里絵  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, 上級研究員 (10399371)
原 尚資  国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 研究員 (20721426)
近藤 康人  藤田医科大学, 医学部, 教授 (30301641)
手島 玲子  岡山理科大学, 獣医学部, 教授 (50132882)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2019: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2018: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2017: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2016: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Keywordsソバ / アレルゲン / 自殖化 / QTL / アレルゲンタンパク質 / 低アレルゲンソバ / アレルギー / 育種 / 食品化学 / エピトープ配列 / 遺伝子領域内変異 / 低アレルゲン系統 / 低アレルゲン個体 / 育種学 / 食物アレルギー / エピトープ変異 / アナフィラキシー
Outline of Final Research Achievements

The aim of this study is to reduce or eliminate allergenic proteins in buckwheat seeds, especially the Fag e 2 protein that causes anaphylactic shock.
As a result, we found diverse mutations within the Fag e 2 gene region in buckwheat genetic resources. It became clearly that the reactivity to anti-Fag e 2 antibody was different among self-lines which were bred ourselves and that the reactivity variation was also hereditary. We generated segregating populations for genetic analysis of anti-Fag e 2 antibody reactivity using strong and weak reactivity individuals among self-lines, and confirmed the genetic segregation of a monomial distribution. This suggests that the abundance of allergenic proteins is regulated by QTL genes.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、他殖性作物のソバにおいて、自家和合性系統群の作製に成功したことである。他殖性ソバ集団内の多様な変異を顕在化させ、選抜・固定し、ゲノムレベルでのプロファイリングおよびアレルゲン性評価を実施できたためアレルゲン低減化品種育成を可能にした。また、アレルゲン反応性の強弱は単一の遺伝子効果ではなく、量的な形質として把握するべきであることを明らかにしたのは本研究が世界でも初めてである。
本研究によってノンアレルゲンソバ品種の育成が可能となり、国内外需要が上昇し、6次産業化が生じること、消費者が安心して食せるようになること、並びに混入・誤食被害が緩和されることが期待される。

Report

(5 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (7 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Development and Evaluation of an Enzyme-Linked Immunosorbent Assay Using a Nonpoisonous Extraction System for the Determination of Crustacean Protein in Processed Foods.2018

    • Author(s)
      Koizumi D, Shirota K, Oda H, Adachi R, Sakai S, Akiyama H, Nishimaki-Mogami T, Teshima R.
    • Journal Title

      J AOAC Int.

      Volume: 101 Issue: 3 Pages: 798-804

    • DOI

      10.5740/jaoacint.17-0324

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 食物アレルゲンコンポーネント2017

    • Author(s)
      近藤康人
    • Journal Title

      栄養

      Volume: 2 Pages: 215-223

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] ソバにおけるタンパク質群の多様性2017

    • Author(s)
      佐藤里絵、原 尚資、山内美月、手島玲子、大澤 良
    • Organizer
      日本食品化学学会
    • Place of Presentation
      伊勢志摩ロイヤルホテル・三重県・志摩市
    • Year and Date
      2017-06-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] そばにおけるタンパク質群の多様性2017

    • Author(s)
      佐藤里絵、原尚資、山内実月、手島玲子、大澤良
    • Organizer
      日本食品化学学会第23回総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Diversity of seed proteins, including allergens, in common buckwheat.2017

    • Author(s)
      Satoh R., Hara T., Teshima R., Ohsawa R.
    • Organizer
      RecentAdvanced in Food Analysis
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ソバ主要アレルゲンFag e 2の遺伝子領域内変異と抗原抗体反応性変異の探索2016

    • Author(s)
      山内美月、原 尚資、佐藤里絵、向井仁美、大澤 良
    • Organizer
      日本育種学会第130回講演会
    • Place of Presentation
      鳥取大学・鳥取県・鳥取市
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Book] Proteomics in Food Science. From Farm to Fork 1st Edition, Chapter 28. Proteomic approaches for allergen analysis in crop plants.2017

    • Author(s)
      Satoh R. and Teshima R.
    • Total Pages
      538
    • Publisher
      Elsevier
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2023-01-30  

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