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Cell dynamics of heat-induced rice chalky formation and nitrogen-enhanced suppression unveiled by using newly-developed on-site cell-specific analysis

Research Project

Project/Area Number 16H02533
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Crop production science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Wada Hiroshi  国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 上級研究員 (40533146)

Co-Investigator(Kenkyū-buntansha) 野並 浩  愛媛大学, 農学研究科, 教授 (00211467)
平岡 賢三  山梨大学, クリーンエネルギー研究センター, 特命教授 (80107218)
恩田 弥生  愛媛大学, 農学研究科, 准教授 (70368463)
中島 大賢  北海道大学, 農学研究院, 助教 (70710945)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥40,690,000 (Direct Cost: ¥31,300,000、Indirect Cost: ¥9,390,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2016: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Keywordsイネ / 高温登熟 / 1細胞代謝産物解析 / 背白米 / 高温ストレス / 細胞計測 / タンパク質 / 浸透圧調節 / 水稲 / 品種間差 / 作物学 / 細胞分子計測 / 温暖化 / 水稲玄米品質 / 窒素施肥 / 浸透調節 / タンパク質合成
Outline of Final Research Achievements

Heat-induced chalky formation is a major concern in rice production. The chalkiness is suppressed by nitrogen, yet little is known about the cellular dynamics. Picolitre pressure-probe electrospray-ionization mass spectrometry and transmission electron microscopy were used to investigate the cellular mechanisms of both processes in single endosperm cells of intact growing plants. We found an enlargement of protein storage vacuoles in osmotically adjusted cells under heat conditions with little accumulation of storage proteins prior to chalky formation, in addition to the partial inhibition of amyloplast development. Increased nitrogen availability enhanced heat tolerance through strong osmotic adjustment and the cells sustained protein synthesis even under heat conditions, which led to normal storage protein and amyloplast development to suppress air space formation. These findings provide new insight into the nitrogen-associated heat responses in the growing endosperm cells.

Academic Significance and Societal Importance of the Research Achievements

水稲登熟期の高温に伴う白未熟粒(主として背白粒)の発生により,全国的に米品質の低下被害が多発している.これまで,背白粒の発生は窒素追肥により回避できることが知られてきたが,白濁形成・窒素施与による白濁回避が起こる細胞レベルのメカニズムについては研究が進展していなかった.本研究では,近年開発した1細胞代謝産物分析法と透過型電子顕微鏡観察を融合させることにより,高温下の非破壊の玄米胚乳細胞内で何が起き,白濁に至ったのかそのメカニズムを明らかにしている.本成果は稲作現場の技術開発・品種開発につながる基礎知見であり,学術的価値はもとより,社会的なインパクトのある成果である.

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (38 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (17 results) (of which Int'l Joint Research: 11 results,  Peer Reviewed: 17 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 6 results) Book (2 results) Remarks (5 results)

  • [Int'l Joint Research] ヘルシンキ大学/フィンランド自然資源研究所(フィンランド)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice2020

    • Author(s)
      Wada H, Hatakeyama Y, Nakashima T, Nonami H, Erra-Balsells R, Hakata M, Nakata K, Hiraoka K, Onda Y, Nakano H
    • Journal Title

      Scientific Reports

      Volume: 10(1) Issue: 1 Pages: 2013-2013

    • DOI

      10.1038/s41598-020-58869-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ray parenchymal cells contribute to lignification of tracheids in developing xylem of Norway spruce2019

    • Author(s)
      Olga Blokhina, Teresa Laitinen, Yuto Hatakeyama, Nicolas Delhomme, Tanja Paasela, Lei Zhao, Nathaniel R. Street, Hiroshi Wada, Anna Karkonen, Kurt Fagerstedt
    • Journal Title

      Plant Physiology

      Volume: 181(4) Issue: 4 Pages: 1552-1572

    • DOI

      10.1104/pp.19.00743

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Component Profiling in Agricultural Applications Using an Adjustable Acupuncture Needle for Sheath-Flow Probe Electrospray Ionization/Mass Spectrometry2019

    • Author(s)
      Hiraoka Kenzo、Rankin-Turner Stephanie、Ninomiya Satoshi、Wada Hiroshi、Nakano Hiroshi、Matsumura Masaya、Sanada-Morimura Sachiyo、Tanaka Fukuyo、Nonami Hiroshi
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 67 Issue: 11 Pages: 3275-3283

    • DOI

      10.1021/acs.jafc.8b06424

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Point analysis of foods by sheath-flow probe electrospray ionization/mass spectrometry (sfPESI/MS) coupled with a touch sensor2019

    • Author(s)
      Hiraoka K, Rankin-Turner S, Ninomiya S, Sekine R, Wada H, Matsumura M, Sanada-Morimura S, Tanaka F, Nonami H, Ariyada O
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 68(1) Issue: 1 Pages: 418-524

    • DOI

      10.1021/acs.jafc.9b06489

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Multiple strategies for heat adaptation to prevent chalkiness in the rice endosperm2019

    • Author(s)
      Wada H, Hatakeyama Y, Onda Y, Nonami H, Nakashima T, Erra-Balsells R, Morita S, Hiraoka K, Tanaka F, Nakano H
    • Journal Title

      Journal of Experimental Botany

      Volume: 70(4) Issue: 4 Pages: 1299-1311

    • DOI

      10.1093/jxb/ery427

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evidence for preservation of vacuolar compartments during foehn-induced chalky ring formation of Oryza sativa L.2018

    • Author(s)
      Hatakeyama Y, Masumoto-Kubo C, Nonami H, Morita S, Hiraoka K, Onda Y, Nakashima T, Nakano N, and Wada H
    • Journal Title

      Planta

      Volume: 248(5) Issue: 5 Pages: 1263-1275

    • DOI

      10.1007/s00425-018-2975-x

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electrospray Generated from the Tip-Sealed Fine Glass Capillary Inserted with an Acupuncture Needle Electrode2018

    • Author(s)
      Dilshadbek T. Usmanov, Satoshi Ninomiya, Kenzo Hiraoka, Hiroshi Wada, Hiroshi Nakano, Masaya Matsumura, Sachiyo Sanada-Morimura, and Hiroshi Nonami
    • Journal Title

      Journal of The American Society for Mass Spectrometry

      Volume: 29 Issue: 12 Pages: 2297-2304

    • DOI

      10.1007/s13361-018-2062-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dipping probe electrospray ionization/mass spectrometry for direct on-site and low-invasive food analysis2018

    • Author(s)
      Usmanov Dilshadbek T.、Mandal Mridul K.、Hiraoka Kenzo、Ninomiya Satoshi、Wada Hiroshi、Matsumura Masaya、Sanada-Morimura Sachiyo、Nonami Hiroshi、Yamabe Shinichi
    • Journal Title

      Food Chemistry

      Volume: 260 Pages: 53-60

    • DOI

      10.1016/j.foodchem.2018.04.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Remote sampling mass spectrometry for dry samples: Sheath-flow probe electrospray ionization (PESI) using a gel loading tip inserted with an acupuncture needle2018

    • Author(s)
      Dilshadbek T. Usmanov, Khatam B. Ashurov, Satoshi Ninomiya, Kenzo Hiraoka, Hiroshi Wada, Hiroshi Nakano, Masaya Matsumura, Sachiyo Sanada-Morimura, Hiroshi Nonami
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 32 Issue: 5 Pages: 407-413

    • DOI

      10.1002/rcm.8045

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Turgor-responsive starch phosphorylation in Oryza sativa stems: a primary event of starch degradation associated with grain-filling ability2017

    • Author(s)
      3.Wada H, Masumoto-Kubo C, Tsutsumi K, Nonami H, Tanaka F, Okada H, Erra-Balsells R, Hiraoka K, Nakashima T, Hakata M, and Morita S
    • Journal Title

      PLOS ONE

      Volume: 12(7) Issue: 7 Pages: e0181272-e0181272

    • DOI

      10.1371/journal.pone.0181272

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analysis of fluorene and 9,9-dialkylfluorenes by electrospray droplet impact (EDI)/SIMS2017

    • Author(s)
      Rio Takaishi, Dilshadbek Tursunbayevich Usmanov, Satoshi Ninomiya, Yuji Sakai, Kenzo Hiraoka, Hiroshi Wada, Masaya Matsumura, Sachiyo Sanada-Morimura, Hiroshi Nonami, Shinichi Yamabe
    • Journal Title

      International Journal of Mass Spectrometry

      Volume: 419 Pages: 29-36

    • DOI

      10.1016/j.ijms.2017.05.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pulsed probe electrospray and nano-electrospray: the temporal profiles of ion formation from the Taylor cone2017

    • Author(s)
      Dilshadbek T. Usmanov, Kenzo Hiraoka, Satoshi Ninomiya, Lee Chuin Chen, Hiroshi Wada, Masaya Matsumura, Sachiyo Sanada-Morimura, Keisuke Nakata Hiroshi Nonami
    • Journal Title

      Analytical Methods

      Volume: 9 Issue: 34 Pages: 4958-4963

    • DOI

      10.1039/c7ay01275f

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Non-proximate mass spectrometry using a heated 1-m long PTFE tube and an air-tight APCI ion source2017

    • Author(s)
      Dilshadbek U, Hiraoka K, Wada H, Matsumura M, Sanada-Morimura S, Nonami H, Yamabe
    • Journal Title

      Analytica Chimica Acta

      Volume: - Pages: 59-67

    • DOI

      10.1016/j.aca.2017.03.044

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Desorption in Mass Spectrometry2017

    • Author(s)
      Dilshadbek U, Ninomiya S, Chen LC, Saha S, Mandal MK, Sakai Y, Takaishi R, Habib A, Hiraoka K, Yoshimura K, Takeda S, Wada H., Nonami H
    • Journal Title

      Mass Spectrometry

      Volume: 6 Issue: 2 Pages: S0059-S0059

    • DOI

      10.5702/massspectrometry.S0059

    • NAID

      130005243790

    • ISSN
      2186-5116, 2187-137X
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gaseous ion formation by the cavitation occurred between aqueous solutions and the ultrasonically vibrating blade studied by mass spectrometry2016

    • Author(s)
      Dilshadbek U, Hiraoka K, Wada H, Matsumura M, Sanada-Morimura S, Nonami H
    • Journal Title

      International J. Mass Spectrometry

      Volume: 411 Pages: 34-39

    • DOI

      10.1016/j.ijms.2016.11.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mass spectrometric monitoring of oxidation of aliphatic C6-C8 hydrocarbons and ethanol in low pressure oxygen and air plasmas2016

    • Author(s)
      Dilshadbek U, Chen LC, Hiraoka K, Wada H, Nonami H, Yamabe S
    • Journal Title

      Journal of Mass Spectrometry

      Volume: 51 Issue: 12 Pages: 1187-1195

    • DOI

      10.1002/jms.3890

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrogen incorporation in saturated aliphatic C6-C8 hydrocarbons and ethanol in low-pressure nitrogen plasma generated by a hollow cathode discharge ion source2016

    • Author(s)
      Dilshadbek U, Chen LC, Hiraoka K, Wada H, Nonami H, Yamabe S
    • Journal Title

      Journal of Mass Spectrometry

      Volume: 51 Issue: 6 Pages: 446-452

    • DOI

      10.1002/jms.3765

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 米生産における白未熟粒の発生メカニズムについて2020

    • Author(s)
      和田博史
    • Organizer
      令和元年度「おいでまい」栽培者研修大会(香川県主催)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] New approaches combined with environmental control for enhancing rice production under heat conditions2019

    • Author(s)
      Hiroshi Wada
    • Organizer
      ヘルシンキ大学Viikki植物科学(ViPS)センター
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 高温登熟環境下での背白米発生及び窒素施用による品質改善機構の品種間差異2018

    • Author(s)
      1.畠山友翔, 中野洋, 恩田弥生, 野並浩, Rosa Erra-Balsells, 中島大賢, 平岡賢三, 和田博史
    • Organizer
      第245回日本作物学会講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 気候変動下における水稲の安定多収生産に向けて:高温障害対策を中心とする研究開発と今後の課題2018

    • Author(s)
      和田博史
    • Organizer
      日本農業気象学会75周年記念大会(2018年全国大会)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 制御環境下の細胞生理学的アプローチの展望:水稲高温障害研究を例として2018

    • Author(s)
      和田博史
    • Organizer
      日本農業気象学会75周年記念大会(2018年全国大会)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Phosphoinositides in rice pollen grains associated with spikelet fertility2018

    • Author(s)
      Wada H, Nakashima T, Hakata M, Hatakeyama Y, Nakata K, Nonami H, Erra-Balsells R, Hiraoka K, and Nakano H
    • Organizer
      2017 CIGR World Workshop in Matsuyama
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of cytoplasmic streaming in osmotic adjustment in tomato trichome cells under water stress2018

    • Author(s)
      Nakata K, Wada H, Onda Y, Erra-Balsells R, and Nonami H
    • Organizer
      2017 CIGR World Workshop in Matsuyama
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of the on-site live cell metabolomics performable in controlled environment2017

    • Author(s)
      Wada H, Nonami H, Tanaka K, Nakashima T, Hatakeyama Y, Nakano H, Onda Y, Hiraoka K, Hakata M, and Morita S
    • Organizer
      Society for Experimental Biology 2017 Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nitrogen application reverses heat-induced rice chalkiness: evidence for organelle rearrengement due to the recovery of protein synthesis in endosperm cells2017

    • Author(s)
      Hatakeyama Y, Onda Y, Nonami H, Nakashima T, Nakano H, Erra-Balsells R, Hiraoka K, Wada H
    • Organizer
      Society for Experimental Biology 2017 Annual Meeting
    • Related Report
      2017 Annual Research Report
  • [Presentation] Pico pressure probe electrospray mass spectrometry for in situ single plant cell qualitative and quantitative analysis of phosphate metabolic pathways2017

    • Author(s)
      Nakata K, Wada H, Erra-Balsells R, Nonami H
    • Organizer
      第65回質量分析総合討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] フェーンに伴うリング状乳白粒の発生機構:オンサイト・セルスペシフィック解析による水稲高温障害研究への応用2016

    • Author(s)
      和田博史,羽方誠,中野洋,野並浩,森田敏
    • Organizer
      日本農業気象学会九州支部・日本生物環境工学会九州支部合同大会シンポジウム
    • Place of Presentation
      鹿児島大学(鹿児島県・鹿児島市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Mechanisms of foehn-induced chalky ring formation in rice: Use of the on-site cell-specific analytical method and practical applications2016

    • Author(s)
      Wada H, Hakata M, Nonami H, Nakano H, and Morita S
    • Organizer
      IRRI-JIRCAS-NARO Symposium, Session I. High temperature tolerance, Session I-1
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-cell metabolite profiling of stalk and glandular cells of intact trichomes with internal electrode capillary pressure probe electrospray ionization mass spectrometry2016

    • Author(s)
      Nonami H, Nakashima T, Wada H, Morita S, Erra-Balsells R, Hiraoka K
    • Organizer
      The 64th Annual Conference on Mass Spectrometry
    • Place of Presentation
      ホテル阪急エクスポパーク(大阪府・大阪市)
    • Year and Date
      2016-05-18
    • Related Report
      2016 Annual Research Report
  • [Book] Adaptation to Climate Change in Agriculture2019

    • Author(s)
      Iizumi, Toshichika, Hirata, Ryuichi, Matsuda, Ryo (Eds.)
    • Total Pages
      228
    • Publisher
      Springer Nature
    • ISBN
      9789811392351
    • Related Report
      2019 Annual Research Report
  • [Book] 米の外観品質・食味 ―最新研究と改善技術―2018

    • Author(s)
      松江勇次編著
    • Total Pages
      490
    • Publisher
      養賢堂
    • ISBN
      484250563X
    • Related Report
      2018 Annual Research Report
  • [Remarks] イネの登熟期高温による背白粒発生と窒素追肥による抑制メカニズムの一端が明らかに!

    • URL

      https://www.naro.affrc.go.jp/publicity_report/publication/files/P2_karc_naro_News_62.pdf

    • Related Report
      2019 Annual Research Report
  • [Remarks] IRRI-JIRCAS-NARO Symposium

    • URL

      http://www.naro.affrc.go.jp/english/global-initiatives/irri-jircas-naro-joint-symposiumosium/program.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 日本農業気象学会九州支部・日本生物環境工学会九州支部合同大会シンポジウム

    • URL

      http://www2.bpes.kyushu-u.ac.jp/~www-met/kyushubr2016/sympo.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] The 64th Annual Conference on Mass Spectrometry

    • URL

      http://www.mssj.jp/conf/64/program/1P-08.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 九州沖縄農業研究センター 水田作研究領域

    • URL

      http://www.naro.affrc.go.jp/karc/introduction/chart/suiden_engei_area/saibai/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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