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Study on mechanisms of pathogenicity divergence in Fusarium oxysporum by structural and functional analyses of small chromosomes

Research Project

Project/Area Number 16H02536
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant protection science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

ARIE Tsutomu  東京農工大学, (連合)農学研究科(研究院), 教授 (00211706)

Co-Investigator(Kenkyū-buntansha) 飯田 祐一郎  国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 主任研究員 (00456609)
荒添 貴之  東京理科大学, 理工学部応用生物科学科, 助教 (40749975)
小松 健  東京農工大学, (連合)農学研究科(研究院), 准教授 (60451837)
川部 眞登  富山県農林水産総合技術センター, 富山県農林水産総合技術センター園芸研究所, 主幹研究員 (60462678)
赤木 靖典  鳥取大学, (連合)農学研究科(研究院), プロジェクト研究員 (60626589)
Research Collaborator KASHIWA Takeshi  
AYUKAWA Yu  
SAITO Hiroki  
KOTERA Syunsuke  
AKAI Kotaro  
JO Seong-Mi  
HANYUDA Saeri  
KATO Yukiko  
SAKAI Misa  
KAMAKURA Takashi  
TURGEON B Gillian  
YUN Sung-Hwan  
REP Martijn  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥40,950,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥9,450,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2017: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2016: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Keywordsフザリウム / 小型染色体 / 宿主特異性 / 病原性分化 / トマト萎凋病
Outline of Final Research Achievements

Phytopathogenic Fusarium oxysporum is differentiated in virulence like formae speciales based on a range of plant species which can be the host, and races based on a range cultivars which can be the host. Although this differentiation is very clear, the mechanism of differentiation has been unknown. Recently, it has been reported that F. oxysporum may have a small chromosome that is not essential for growth, and this small chromosome is involved in virulence. In this study, analyses and comparison of genes and structures on small chromosomes of F. oxysporum isolates with different virulence, suggesting that some genes may be involved in virulence, host specificity determination, race determination, etc. We obtained basic knowledge for elucidating pathogenic differentiation mechanisms such as forms and races.

Academic Significance and Societal Importance of the Research Achievements

植物病原性Fusarium oxysporumの分化型やレースの未詳な分化機構を、小型染色体の解析を通して明らかにすることができる可能性を示した。これは、これまでの病原と植物(抵抗性品種)のいたちごっこのメカニズム解析および小型染色体の寄与の解明につながり、植物病理学的に興味深いばかりでなく、今後の育種などによる病害防除のヒントになり、社会的意義がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (45 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (14 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 13 results,  Open Access: 6 results,  Acknowledgement Compliant: 4 results) Presentation (26 results) (of which Int'l Joint Research: 7 results,  Invited: 6 results) Book (1 results)

  • [Int'l Joint Research] University of Agriculture at La Molina(ペルー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Can Tho University(ベトナム)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ワーヘニンゲン大学(オランダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Cornell University(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Induction of resistance to diseases in plant by aerial ultrasound irradiation2019

    • Author(s)
      Kawakami D, Yoshida T, Kanemaru Y, Heidi M, Zaquinaula H, Mizukami T, Arimoto M, Shibata T, Goto A, Enami Y, Amano H, Teraoka T, Komatsu K, Arie T
    • Journal Title

      Journal of Pesticide Science

      Volume: 44 Issue: 1 Pages: 41-47

    • DOI

      10.1584/jpestics.D18-064

    • NAID

      130007602389

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2019-02-20
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A putative RNA silencing component protein FoQde-2 is involved in virulence of the tomato wilt fungus Fusarium oxysporum f. sp. lycopersici2018

    • Author(s)
      Jo S-M, Ayukawa Y, Yun S-H, Komatsu K, Arie T
    • Journal Title

      J Gen Plant Pathol

      Volume: 84 Issue: 6 Pages: 395-398

    • DOI

      10.1007/s10327-018-0800-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)2018

    • Author(s)
      Ayukawa Y, Komatsu K, Taga M, Arie T
    • Journal Title

      J Gen Plant Pathol

      Volume: 84 Issue: 4 Pages: 254-261

    • DOI

      10.1007/s10327-018-0784-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chrysoviruses in Magnaporthe oryzae2018

    • Author(s)
      Moriyama H, Urayama SI, Higashiura T, Le TM, Komatsu K
    • Journal Title

      Viruses

      Volume: 10 Issue: 12 Pages: 697-710

    • DOI

      10.3390/v10120697

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Panama disease of banana occurred in Miyakojima Island, Okinawa, Japan2018

    • Author(s)
      Nitani Takao、Akai Kotaro、Hasegawa Ryo、Ayukawa Yu、Garcia Ricardo Romero、Chitose Atsushi、Komatsu Ken、Kikuno Hidehiko、Natsuaki Keiko T.、Arie Tsutomu
    • Journal Title

      Journal of General Plant Pathology

      Volume: 84 Issue: 2 Pages: 165-168

    • DOI

      10.1007/s10327-018-0769-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Knockout of the SREBP system increases production of the polyketide FR901512 in filamentous fungal sp. No. 14919 and lovastatin in Aspergillus terreus ATCC205422018

    • Author(s)
      Itoh Hiroya、Miura Ai、Matsui Makoto、Arazoe Takayuki、Nishida Keiji、Kumagai Toshitaka、Arita Masanori、Tamano Koichi、Machida Masayuki、Shibata Takashi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 102 Issue: 3 Pages: 1393-1405

    • DOI

      10.1007/s00253-017-8685-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 分解酵素とインヒビターを介した植物と病原菌の攻防-植物病原菌の矛と盾2018

    • Author(s)
      飯田祐一郎
    • Journal Title

      化学と生物

      Volume: 56 Pages: 244-245

    • NAID

      130007616817

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi2017

    • Author(s)
      Ayukawa Yu、Hanyuda Saeri、Fujita Naoko、Komatsu Ken、Arie Tsutomu
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 4253-4253

    • DOI

      10.1038/s41598-017-04084-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species2017

    • Author(s)
      van Dam Peter、Fokkens Like、Ayukawa Yu、van der Gragt Michelle、ter Horst Anneliek、Brankovics Bal?zs、Houterman Petra M.、Arie Tsutomu、Rep Martijn
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 9042-9042

    • DOI

      10.1038/s41598-017-07995-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] L-Methionine induces resistance to Fusarium wilt of tomato plants.2017

    • Author(s)
      齋藤まどか・中島 雅己・有江 力・阿久津 克己
    • Journal Title

      Japanese Journal of Phytopathology

      Volume: 83 Issue: 1 Pages: 3-9

    • DOI

      10.3186/jjphytopath.83.3

    • NAID

      130005492457

    • ISSN
      0031-9473, 1882-0484
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequencing of individual chromosomes of plant pathogenic Fusarium oxysporum2017

    • Author(s)
      Kashiwa T, Kozaki T, Ishii K, Turgeon BG, Teraoka T, Komatsu K, Arie T
    • Journal Title

      Fungal Genet Biol

      Volume: 98 Pages: 46-51

    • DOI

      10.1016/j.fgb.2016.12.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A new biotype of Fusarium oxysporum f. sp. lycopersici race 2 emerged by a transposon-driven mutation of avirulence gene AVR12016

    • Author(s)
      Kashiwa T, Suzuki T, Sato A, Akai K, Teraoka T, Komatsu K, Arie T
    • Journal Title

      FEMS Microbiol Lett

      Volume: 77 Issue: 14 Pages: 371-383

    • DOI

      10.1093/femsle/fnw132

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Detection and differentiation of Fusarium oxysporum f. sp. lycopersici race 1 using loop-mediated isothermal amplification with three primer sets2016

    • Author(s)
      Ayukawa Y, Komatsu K, Kashiwa T, Akai K, Yamada M, Teraoka T, Arie T
    • Journal Title

      Lett Appl Microbiol

      Volume: 63 Issue: 3 Pages: 202-209

    • DOI

      10.1111/lam.12597

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Detection of cabbage yellows fungus Fusarium oxysporum f. sp. conglutinans in soil by PCR and real-time PCR2016

    • Author(s)
      Kashiwa T, Inami K, Teraoka T, Komatsu K, Arie T
    • Journal Title

      J Gen Plant Pathol

      Volume: 82 Issue: 5 Pages: 240-247

    • DOI

      10.1007/s10327-016-0668-5

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Study on Fusarium oxysporum f. sp. cubense in Japan2019

    • Author(s)
      Arie T, Nitani T, Kotera S, Tsuzuki R
    • Organizer
      International banana wilt disease workshop at Piura
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] フザリウムによる植物病害の防除と検診,分子遺伝学に関する研究2019

    • Author(s)
      有江 力
    • Organizer
      第44回日本農薬学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ミツバおよびコリアンダーから分離したFusarium oxysporumの病原性および分子系統解析2019

    • Author(s)
      加藤有紀子・小松 健・有江 力
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キョウナ萎凋病から分離したFusarium oxysporum株とアブラナ科を宿主とする分化型菌株の比較2019

    • Author(s)
      酒井美沙・宮本拓也・中島雅己・小松 健・有江 力
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Spraying rice flowers with a non-pathogenic Fusarium oxysporum W5 effectively controls “Bakanae” by competition with the pathogen in planta2018

    • Author(s)
      Saito H, Komatsu K, Arie T
    • Organizer
      14th European Fusarium Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diversity in the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici: variation of mutations in AVR genes in the field isolates2018

    • Author(s)
      Arie T, Hanyuda S, Masuko R, Ayukawa Y, Akai K, Komatsu K
    • Organizer
      14th European Fusarium Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analyses of RecQ helicase and SMS-2 protein genes in Fusarium oxysporum f. sp. lycopersici2018

    • Author(s)
      Jo S-M, Komatsu K, Yun S-H Arie T
    • Organizer
      14th European Fusarium Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 土壌伝染性Fusarium oxysporumの病原性進化・分化のメカニズムと多様性: その知見を土壌検診に活かす2018

    • Author(s)
      有江 力
    • Organizer
      日本土壌微生物学会2018年度広島大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 非病原性Fusarium oxysporum W5ゲノムはコア染色体でない領域を有する2018

    • Author(s)
      齊藤大幹・浅井秀太・小松 健・有江 力
    • Organizer
      第18回糸状菌分子生物学コンファレンス
    • Related Report
      2018 Annual Research Report
  • [Presentation] Fusarium oxysporumの分子生物学的・ ゲノム科学的特徴とそれに基づく エンドウ萎凋病菌の特異識別2018

    • Author(s)
      有江 力・小寺俊丞
    • Organizer
      植物防疫官技術研修会ゼミナ-ル
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] キャベツ萎黄病菌Fusarium oxysporum f. sp. conglutinansの複数菌株がホウレンソウに病原性を示す2018

    • Author(s)
      小寺俊丞・小松 健・有江 力
    • Organizer
      平成30年度日本植物病理学会関東部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 生物防除資材候補である非病原性Fusarium oxysporum W5のゲノム解析2018

    • Author(s)
      齊藤大幹・浅井秀太・小寺俊丞・小松 健・有江 力
    • Organizer
      平成30年度日本植物病理学会関東部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キャベツ萎黄病菌Cong:1-1株のキャベツ,シロイヌナズナ,ストックに対する病原性を司る小型染色体は異なる2018

    • Author(s)
      鮎川 侑・小松 健・有江 力
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Functional analyses of RecQ helicase and SMS-2 protein genes in Fusarium oxysporum f. sp. lycopersici2018

    • Author(s)
      Jo S, Komatsu K, Yun SH, Arie T
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高知県の圃場におけるトマト萎凋病制御後の萎凋病菌の経時的消長2018

    • Author(s)
      羽生田冴莉・鮎川 侑・森田泰彰・山崎睦子・小松 健・有江 力
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] エンドウ萎凋病菌Fusarium oxysporum f. sp. pisiの分子系統解析と特異検出法の検討2018

    • Author(s)
      小寺俊丞・菱池政志・小松 健・有江 力
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] チューリップ球根腐敗病菌の病原性関連SIX遺伝子群の検出2018

    • Author(s)
      川部眞登
    • Organizer
      平成29年度関東東海北陸農業試験研究推進会議 北陸・水田農業部会 病害 虫害技術研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Diversity of mutations in AVR1 and AVR2 genes in the field isolates of tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici2017

    • Author(s)
      Akai K, Tamura S, Kikuchi, S, Ayukawa Y, Akagi Y, Komatsu K, Kodama M
    • Organizer
      29th Fungal Genetics Conference
    • Place of Presentation
      Asilomar, CA, USA
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fusarium oxysporum f. sp. conglutinansの小型染色体は宿主植物によって病原的機能が異なる2017

    • Author(s)
      鮎川 侑・小松 健・Martijn Rep・有江 力
    • Organizer
      平成29年度日本植物病理学会植物感染生理談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トマト萎凋病菌レース1 MAFF 103036株の小型染色体喪失株のトマトへの病原力2017

    • Author(s)
      増子諒一・鮎川 侑・小松 健・有江 力
    • Organizer
      平成29年度日本植物病理学会関東部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] チューリップ球根腐敗病菌の系統と薬剤抵抗性・病原性に関して2017

    • Author(s)
      川部眞登
    • Organizer
      第11回フザリウム研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] チューリップ球根腐敗病菌のSIX遺伝子群の検出2017

    • Author(s)
      川部眞登・有江力
    • Organizer
      第17回糸状菌分子生物学カンファレンス
    • Related Report
      2017 Annual Research Report
  • [Presentation] Functional analysis of an Argonaute-like protein FoQDE in Fusarium oxyspsorum f. sp. lycopersici2016

    • Author(s)
      Jo S-M, Yun S-H, Komatsu K,
    • Organizer
      第16回糸状菌分子生物学コンファレンス
    • Place of Presentation
      京都大学、宇治市、京都府
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 発芽管破裂法(GTBM)によるFusarium oxysporumの細胞学的核型解析2016

    • Author(s)
      鮎川侑・小松 健・多賀正節・有江 力
    • Organizer
      第16回糸状菌分子生物学コンファレンス
    • Place of Presentation
      京都大学、宇治市、京都府
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Diversity of pathogenicity of the tomato wilt fungus2016

    • Author(s)
      Arie T
    • Organizer
      Amsterdam University Seminar
    • Place of Presentation
      Amsterdam, The Netherlands
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Variations in avirulence genes on small chromosomes in the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici; emergence of new biotypes, rapid race determination methods, and control of the disease2016

    • Author(s)
      Arie T
    • Organizer
      The 2016 KSPP Spring Meeting and International Conference
    • Place of Presentation
      Jeonju, Korea
    • Year and Date
      2016-04-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 図解でよくわかる 病害虫のきほん: 病害虫発生のメカニズムから、栽培管理、農薬・肥料の使い方、防除法まで (すぐわかるすごくわかる!)2016

    • Author(s)
      有江力
    • Total Pages
      159
    • Publisher
      誠文堂新光社
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2022-10-07  

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