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Studies on the molecular basis of chitooligosaccharide signaling in the arbuscular mycorrhizal symbiosis

Research Project

Project/Area Number 16H02548
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionOsaka Prefecture University

Principal Investigator

Akiyama Kohki  大阪府立大学, 生命環境科学研究科, 教授 (20285307)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000)
Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Keywordsアーバスキュラー菌根菌 / Mycファクター / キトオリゴ糖 / ストリゴラクトン
Outline of Final Research Achievements

AM fungi had long been postulated to produce signal molecules called “Myc factors” that induce the molecular and cellular responses leading to successful root colonization by AM fungi. Lipochitooligosaccharides called Myc-LCOs and small chitin oligomers have been identified as Myc factors from the germinated spore exudates of the AM fungus Rhizophagus irregularis (Maillet et al., 2011, Genre et al., 2012). However, it was found that Myc-LCOs and chitin oligomers elicit only partial and limited induction of symbiotic responses in AM host plants. We previously showed that partially N-deacetylated chitin (DAC) trimers were strong inducers of AM symbiosis-related gene expression in rice roots and were secreted from R. irregularis. There are six possible isomers for DAC trimers. In this study, we identified a DAC trimer isomer, secreted by the AM fungus, acting as an inducer of AM-symbiotic responses in rice.

Academic Significance and Societal Importance of the Research Achievements

植物は共生菌や病原菌に由来する様々なキチン質オリゴ糖を識別して適切な共生・防御応答を示すと考えられている。生理活性オリゴ糖では鎖長が重要な構造的要素であり、一般に4糖以上のものが強い活性を示す。本研究で同定した短鎖のヘテロキトオリゴ糖やキトサンオリゴ糖は3糖が最も強い活性を示す。このような短鎖長のオリゴ糖の共生における関与は全く新規の発見であり、植物微生物相互作用研究において新たな展開をもたらすことが期待される。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2020 2019 2018 2017

All Presentation (5 results)

  • [Presentation] ヘテロキトオリゴ糖受容体のタンパク質同定に向けた光アフィニティープローブの開発2020

    • Author(s)
      廣岡 健太朗、野島 耕陽、秋山 康紀
    • Organizer
      日本農芸化学会大会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヘテロキトオリゴ糖受容体のタンパク質同定に向けた光アフィニティープローブの開発2019

    • Author(s)
      廣岡 健太朗,野島 耕陽,秋山 康紀
    • Organizer
      植物微生物研究会 第29回研究交流会
    • Related Report
      2019 Annual Research Report
  • [Presentation] AM菌が分泌する部分N-脱アセチルキチン3糖による イネ菌根共生遺伝子の発現誘導2018

    • Author(s)
      野島耕陽、秋山康紀
    • Organizer
      植物微生物研究会 第28回研究交流会
    • Related Report
      2018 Annual Research Report
  • [Presentation] AM菌が分泌するヘテロキトオリゴ糖共生シグナルの糖鎖配列の決定2018

    • Author(s)
      野島耕陽、秋山康紀
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 有機合成アプローチによる Myc factor活性へテロキトオリゴ糖の同定2017

    • Author(s)
      野島耕陽、辰巳雄亮、秋山康紀
    • Organizer
      植物微生物研究会 第27回研究交流会
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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