| Project/Area Number |
16H02584
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Tokai University (2018-2019, 2021)
The University of Tokyo (2016-2017) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
草間 和哉 東京薬科大学, 東京薬科大学薬学部, 助教 (30579149)
櫻井 敏博 東京理科大学, 薬学部薬学科, 助教 (70568253)
中尾 洋一 早稲田大学, 理工学術院, 教授 (60282696)
李 俊佑 東京大学, 大学院農学生命科学研究科(農学部), 助教 (10313082)
永岡 謙太郎 東京農工大学, (連合)農学研究科(研究院), 准教授 (60376564)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2019: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Keywords | 着床・胎盤形成 / 上皮間葉系転換 / 細胞融合 / トロホブラスト細胞 / 子宮内環境 / 受胎率向上 / 反芻動物 / 子宮内膜 / 遺伝子発現 / タンパク質発現 / 着床 / 妊娠成立 / 構築 / 再構築 |
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| Outline of Final Research Achievements |
In ruminants, trophoblast cells go through epithelial-mesenchymal transition (EMT) when implanting to the maternal endometrium. As EMT begins, trophoblast cells fuse each other to generate bi-nucleate or multi-nucleated cells, from which pregnancy associated glycoprotein is released. In addition, exosomes, miRNA and lncRNA were found from uterine flushing media and their functions were examined using in vitro uterine epithelial mono-culture or uterine epithelial-trophoblast co-culture systems. miRNA and lncRNA are effective in controling maternal immune system for semi-allogenic trophoblast cells.
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| Academic Significance and Societal Importance of the Research Achievements |
この４年間、反芻動物の着床研究では世界をリードし続けることが出来た。特に、遺伝子・タンパク質発現及びエクソソーム・miRNAやlncRNA分野では先端の研究を続けるだけではなく論文を出し続けた。そのうちの1つ（業績論文9番目）のContinuous model of conceptus implantation to the maternal endometrium.はJ.Endocrinologyで世界で5番目にダウンロードされ、2017年、生殖分野で最も引用回数の多い論文となった。
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