• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Synthetic regulatory mechanism and physiological function of nitric oxidce in yeasts and fungi in

Research Project

Project/Area Number 16H02601
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

TAKAGI Hiroshi  奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (50275088)

Co-Investigator(Kenkyū-buntansha) 那須野 亮  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (90708116)
渡辺 大輔  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (30527148)
川本 進  千葉大学, 真菌医学研究センター, 客員教授 (80125921)
萩原 大祐  筑波大学, 生命環境系, 准教授 (20612203)
知花 博治  千葉大学, 真菌医学研究センター, 准教授 (30333488)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2018: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2017: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2016: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Keywords一酸化窒素 / 酵母 / 酸化ストレス / シグナル伝達 / 病原真菌 / 糸状菌 / 合成制御機構 / 生理機能 / 応用微生物 / 微生物
Outline of Final Research Achievements

We found a candidate gene encoding an enzyme involved in NADPH synthesis, which is required for the NOS reaction. We also showed that an NADPH-dependent diflavin oxidoreductase 1 (NDOR1) and its interaction partner protein CIAPIN1, which are homologous to Tah18 and Dre2, respectively, are involved in the NOS activity in a manner similar to Tah18-Dre2, suggesting that the Tah18-dependent NO synthesis and its regulatory system are conserved in eukaryotic cells from yeasts to mammals. To identify S-nitrosylated proteins in yeast, we developed the biotin switch method combined with LC-MS. Using cell extract treated with a NO donor S-nitrosoglutathione, we identified many S-nitrosylated proteins. In research of pathogenic yeasts and fungi, we found that some of multidrug-resistant strains of Candida species and clinical strains of Aspergillus fumigatus had lower levels of NO or less sensitivity to NO than those of other strains, suggesting that NO is related to pathogenicity.

Academic Significance and Societal Importance of the Research Achievements

酵母のNO合成に関わるTah18タンパク質の哺乳類オルソログ(Ndor1)が酵母のNO合成に寄与することが判明し、酵母で見出したNOの合成制御機構が哺乳類にも保存されている可能性を見出した。また、酵母におけるNOの機能には、ヒトの心臓血管系でも報告されている細胞保護・細胞死の二面性があることが分かった。以上の結果から、酵母で見出したNOの合成制御機構、生理機能を解析することで、哺乳類のNO研究にインパクトを与えるとともに、産業酵母の育種や抗真菌剤の開発など基礎・応用の両面で波及効果が期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (37 results)

All 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (30 results) (of which Int'l Joint Research: 15 results,  Invited: 8 results)

  • [Journal Article] Heterogeneity in Pathogenicity-related Properties and Stress Tolerance in <i>Aspergillus fumigatus</i> Clinical Isolates2018

    • Author(s)
      Hagiwara D, Takahashi H, Takagi H, Watanabe A, Kamei K
    • Journal Title

      Medical Mycology Journal

      Volume: 59 Issue: 4 Pages: E63-E70

    • DOI

      10.3314/mmj.18-00007

    • NAID

      130007538275

    • ISSN
      2185-6486, 2186-165X
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitric oxide signaling in yeast2018

    • Author(s)
      Rika Indri Astuti, Ryo Nasuno, Hiroshi Takagi
    • Journal Title

      Advamces in Microbial Physiology

      Volume: 72 Pages: 29-63

    • DOI

      10.1016/bs.ampbs.2018.01.003

    • ISBN
      9780128144138
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 酵母における一酸化窒素シグナルを介したストレス応答機構2018

    • Author(s)
      那須野 亮, 吉川雄樹, 高木博史
    • Journal Title

      生化学

      Volume: 90 Pages: 701-705

    • NAID

      40021722783

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 酵母における一酸化窒素の分子機能と応用2017

    • Author(s)
      高木博史
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 75 Pages: 214-218

    • NAID

      40021224716

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 酵母に見出した一酸化窒素(NO)の合成制御機構と生理機能2017

    • Author(s)
      那須野 亮, 吉川雄樹, 高木博史
    • Journal Title

      化学と生物

      Volume: 55 Pages: 617-623

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast2016

    • Author(s)
      Yoshikawa Y, Nasuno R, Kawahara N, Nishimura A, Watanabe D, Takagi H.
    • Journal Title

      Nitric Oxide-Biol. Chem.

      Volume: 57 Pages: 85-91

    • DOI

      10.1016/j.niox.2016.04.003

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitric oxide signaling in yeast.2016

    • Author(s)
      Rika Indri Astuti, Ryo Nasuno, Hiroshi Takagi
    • Journal Title

      Applied microbiology and biotechnology.

      Volume: 100 Issue: 22 Pages: 9483-9497

    • DOI

      10.1007/s00253-016-7827-7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Diversity of Aspergillus fumigatus clinical strains in the potential pathogenicity-related properties2018

    • Author(s)
      Daisuke, Hagiwara, Caitlin H. Kowalski, Robert A. Cramer, Hiroki Takahashi, Hiroshi Takagi, Akira Watanabe, Katsuhiko Kamei
    • Organizer
      The 8th Advances Against Aspergillosis conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酵母に見出したフラボタンパク質Tah18依存的な一酸化窒素合成の制御機構と細胞死誘導2018

    • Author(s)
      吉川雄樹, 那須野 亮, 高木博史
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 酵母に見出したフラボタンパク質Tah18依存的な一酸化窒素の合成制御機構の解析2018

    • Author(s)
      吉川雄樹, 那須野亮, 高木博史
    • Organizer
      第18回日本NO学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 酵母におけるS-ニトロソ化タンパク質の同定2018

    • Author(s)
      示野誠也, 吉川雄樹, 那須野 亮, 高木博史
    • Organizer
      第18回日本NO学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 酵母におけるS-ニトロソ化タンパク質の探索・同定・機能解析2018

    • Author(s)
      示野誠也, 吉川雄樹, 那須野 亮, 高木博史
    • Organizer
      第65回日本生化学会近畿支部例会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Novel stress tolerant mechanisms of yeast and their application to biotechnology2018

    • Author(s)
      Hiroshi Takagi
    • Organizer
      The 6th International Conference on Biochemistry and Molecular Biology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast2018

    • Author(s)
      Yuki Yoshikawa, Ryo Nasuno, Hiroshi Takagi
    • Organizer
      The 13th lnternational Meeting on Yeast Apoptosis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酵母に見出した新規な一酸化窒素合成の制御機構の解析2018

    • Author(s)
      吉川 雄樹、那須野 亮、高木 博史
    • Organizer
      酵母遺伝学フォーラム第51回研究報告会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Saccharomyces cerevisiaeにおけるS-ニトロソ化タンパク質の同定・機能解析2018

    • Author(s)
      示野誠也, 吉川雄樹, 那須野 亮, 高木博史
    • Organizer
      酵母遺伝学フォーラム第51回研究報告会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification and functional analysis of nitrated proteins in the yeast Saccharomyces cerevisiae2018

    • Author(s)
      Supapid Eknikom, Ryo Nasuno, Hiroshi Takagi
    • Organizer
      The 34th International Specialized Symposium on Yeasts
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA-sequencingによる糸状菌の一酸化窒素応答の解析2018

    • Author(s)
      萩原大祐, 二宮章洋, 浦山俊一, 高木博史
    • Organizer
      第18回糸状菌分子生物学コンファレンス
    • Related Report
      2018 Annual Research Report
  • [Presentation] Synthetic Mechanism and Physiological Role of Nitric Oxide in Yeast2017

    • Author(s)
      Hiroshi Takagi
    • Organizer
      Gordon Research Conference: Nitric Oxide
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 酵母に見出したフラボタンパク質Tah18依存的な一酸化窒素の合成制御機構と生理機能解析2017

    • Author(s)
      吉川雄樹, 那須野 亮, 渡辺大輔, 高木博史
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Molecular mechanism of nitrosative stress tolerance in yeast2017

    • Author(s)
      Khairul Anam, Ryo Nasuno, Daisuke Watanabe, Hiroshi Takagi
    • Organizer
      日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification and functional analysis of nitrated protein in yeast2017

    • Author(s)
      Supapid Eknikom, Ryo Nasuno, Hiroshi Takagi
    • Organizer
      日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酵母に見出した「両刃の剣」一酸化窒素の合成機構と生理的役割2017

    • Author(s)
      高木博史
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 酵母に見出したフラボタンパク質Tah18依存的な一酸化窒素合成の制御機構と細胞死誘導機構2017

    • Author(s)
      吉川雄樹, 那須野 亮, 高木博史
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酵母に見出した一酸化窒素の合成制御機構と細胞死誘導メカニズムの解析2017

    • Author(s)
      吉川雄樹, 那須野 亮, 高木博史
    • Organizer
      酵母遺伝学フォーラム第50回研究報告会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Synthetic Mechanism and Physiological Role of Nitric Oxide in Yeast2017

    • Author(s)
      Hiroshi Takagi
    • Organizer
      Gordon Research Conference “Nitric Oxide”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Synthetic mechanism and physiological role of nitric oxide in yeast2016

    • Author(s)
      Hiroshi Takagi
    • Organizer
      The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nitric oxide-mediated antioxidative mechanism in yeast: the transcription factor Mac1-dependent activation of the superoxide dismutase Sod12016

    • Author(s)
      Ryo Nasuno, Miho Aitoku, Yuki Manago, Akira Nishimura, Yu Sasano, Hiroshi Takagi
    • Organizer
      The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast2016

    • Author(s)
      Yuki Yoshikawa, Ryo Nasuno, Hiroshi Takagi
    • Organizer
      The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酵母に見出したフラボタンパク質Tah18依存的な一酸化窒素合成の制御機構2016

    • Author(s)
      吉川雄樹, 那須野 亮, 渡辺大輔, 高木博史
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Stress tolerance mechanisms of baker’s yeast mediated by proline/arginine metabolism2016

    • Author(s)
      Hiroshi Takagi
    • Organizer
      The 14th International Congress on Yeasts
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nitric oxide-mediated antioxidative mechanism in yeast through the activation of the transcription factor Mac12016

    • Author(s)
      Ryo Nasuno, Miho Aitoku, Yuki Manago, Akira Nishimura, Hiroshi Takagi
    • Organizer
      The 14th International Congress on Yeasts
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nitric oxide signaling in oxidative stress response in the fission yeast2016

    • Author(s)
      Rika Indri Astuti, Daisuke Watanabe, Hiroshi Takagi
    • Organizer
      The 14th International Congress on Yeasts
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast2016

    • Author(s)
      Yuki Yoshikawa, Ryo Nasuno, Daisuke Watanabe, Hiroshi Takagi
    • Organizer
      The 14th International Congress on Yeasts
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of nitric oxide detoxification in budding yeast Saccharomyces cerevisiae2016

    • Author(s)
      Khairul Anam, Ryo Nasuno, Daisuke Watanabe, Hiroshi Takagi
    • Organizer
      The 14th International Congress on Yeasts
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel stress-tolerant mechanisms of yeast mediated by proline-arginine metabolism and their applications to industrial yeasts2016

    • Author(s)
      Hiroshi Takagi
    • Organizer
      3rd International Seminar on Science
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 製パン過程におけるパン酵母のストレス耐性機構:プロリン・アルギニン代謝と育種への応用2016

    • Author(s)
      高木博史
    • Organizer
      日本イースト工業会平成28年度技術懇談会
    • Related Report
      2016 Annual Research Report
    • Invited

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi