| Budget Amount *help |
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2018: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2017: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2016: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
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| Outline of Final Research Achievements |
Based on the molecular design strategies of fluorogenic probes capable of detecting target cells with single-cell level resolution, which have been established by this group with using the concept of intramolecular spirocyclization and quinone methide intermediate, we succeeded to develop novel photo-sensitizing probes for ablating beta-galactosidase expressing cells in a single-cell resolution manner. It was also capable to ablate lacZ-positive cells selectively by applying the probe to co-culture of lacZ-positive and negative cells, which could not be realized by so far developed photo-sensitizing probes without forming quinone methide intermediate.
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