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Establishment of laboratory medicine of heparin-induced thrombocytopenia

Research Project

Project/Area Number 16H02637
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionThe University of Tokyo

Principal Investigator

Yatomi Yutaka  東京大学, 医学部附属病院, 教授 (60200523)

Research Collaborator YASUMOTO ATSUSHI  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2018: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywordsヘパリン起因性血小板減少症 / HIT抗体検査 / 機能的HIT抗体検査 / サイトカラシンB / 臨床検査医学 / 臨床 / 内科 / シグナル伝達
Outline of Final Research Achievements

Anti-platelet factor 4 (PF4)/heparin antibodies-induced platelet activation is important for thrombosis in heparin-induced thrombocytopenia (HIT), and we established in vitro functional HIT assay system using mouse anti-PF4/heparin monoclonal antibody (HIT-MoAb).
We have found that platelet activation via the clustering of FcγRIIa involves cytochalasin-sensitive cytoskeletal reorganization and that cytochalasin B (CB) significantly enhanced HIT-MoAb-induced platelet activation, accompanied by platelet aggregation. From a practical viewpoint, CB may be useful for improving the sensitivity of HIT functional assays. We have also developed a facile immunological HIT assay system, which can be applied to IVD in the future.

Academic Significance and Societal Importance of the Research Achievements

ヘパリン起因性血小板減少症(heparin-induced thrombocytopenia; HIT)は,抗血栓薬(抗凝固薬)として広く臨床において使用されているヘパリンの重大な副作用であるが,早期かつ的確な診断が難しいことが多く,その診断手法の向上が望まれている.我々は,本診断における二本柱である免疫学的HIT抗体,機能的HIT抗体の手法の向上につながる成果をあげることができた.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (9 results)

All 2019 2018 2017 2016 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Intelligent Image-Activated Cell Sorting2018

    • Author(s)
      Nitta N et al.
    • Journal Title

      Cell

      Volume: 175 Issue: 1 Pages: 266-276.e13

    • DOI

      10.1016/j.cell.2018.08.028

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-throughput imaging flow cytometry by optofluidic time-stretch microscopy2018

    • Author(s)
      Lei Cheng、Kobayashi Hirofumi、Wu Yi、Li Ming、Isozaki Akihiro、Yasumoto Atsushi、Mikami Hideharu、Ito Takuro、Nitta Nao、Sugimura Takeaki、Yamada Makoto、Yatomi Yutaka、Di Carlo Dino、Ozeki Yasuyuki、Goda Keisuke
    • Journal Title

      Nature Protocols

      Volume: 13 Issue: 7 Pages: 1603-1631

    • DOI

      10.1038/s41596-018-0008-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiplex quantitative analysis of eicosanoid mediators in human plasma and serum: Possible introduction into clinical testing2017

    • Author(s)
      Yasumoto Atsushi、Tokuoka Suzumi M.、Kita Yoshihiro、Shimizu Takao、Yatomi Yutaka
    • Journal Title

      J Chromatogr B Analyt Technol Biomed Life Sci.

      Volume: 1068-1069 Pages: 98-104

    • DOI

      10.1016/j.jchromb.2017.10.014

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] HITの診断を確定させる際に有用な血清学的診断法はどれか?2019

    • Author(s)
      安本篤史
    • Organizer
      第13回日本血栓止血学会SSCシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Regulation of FcγRIIa-mediated platelet activation by a cytochalasin-sensitive cytoskeletal rearrangement: its clinical application to the detection of functional HIT antibody2017

    • Author(s)
      Atsushi Yasumoto
    • Organizer
      International Society on Thrombosis and Haemostasis (ISTH)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of FcγRIIa-mediated platelet activation by a cytochalasin-sensitive cytoskeletal rearrangement: its clinical application to the detection of functional HIT antibody.2017

    • Author(s)
      Atsushi Yasumoto
    • Organizer
      International Society on Thrombosis and Haemostasis (ISTH)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サイトカラシンB前処理血小板を用いたHIT診断のための機能的測定法の有用性2016

    • Author(s)
      安本篤史
    • Organizer
      第11回日本血栓止血学会学術標準化委員会(SSC)シンポジウム
    • Related Report
      2016 Annual Research Report
  • [Book] 臨床に直結する血栓止血学2018

    • Author(s)
      朝倉 英策
    • Total Pages
      688
    • Publisher
      中外医学社
    • ISBN
      4498125797
    • Related Report
      2018 Annual Research Report
  • [Remarks] 東京大学医学部附属病院検査部ホームページ

    • URL

      http://lab-tky.umin.jp/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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