Study on the genetic background of heat stress tolerance in dairy cattle in Southeast Asia

• Project/Area Number: 16H02766
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Animal production science
• Research Institution: Nagoya University
• Principal Investigator: Ohkura Satoshi 名古屋大学, 生命農学研究科, 教授 (20263163)
• Co-Investigator(Kenkyū-buntansha): 松田 二子 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (10608855) ; PHENG Vutha 名古屋大学, アジアサテライトキャンパス学院(農), 特任准教授 (70747120)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000); Fiscal Year 2019: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2016: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
• Keywords: 応用動物 / 獣医学 / 畜産学 / 神経科学 / ストレス / ウシ / 暑熱 / 心拍変動

Outline of Final Research Achievements

The present study aimed to identify the characteristics of heat stress tolerance by physiological and genetic analysis and to elucidate the mechanism of heat tolerance in dairy cattle raised in Southeast Asia. Using a Holter-type electrocardiograph to analyze heart rate variability in dairy cattle raised on farms in Cambodia, the frequency domain indices of heart rate variability were found to be used to select individuals with heat tolerance. We also conducted GRAS-Di analysis with next-generation sequencing on these dairy cows to conduct a comprehensive polymorphism analysis of candidate genes that confer heat stress tolerance.

Academic Significance and Societal Importance of the Research Achievements

本研究において確立した、心拍変動解析による自律神経機能の変化に着目してウシの暑熱ストレス反応を評価する手法は、暑熱環境下にあるウシの暑熱ストレスへの適応度の差違を判別するツールとして活用できる。今後、心拍変動の周波数成分パラメータを用いて暑熱ストレス耐性を有する乳用牛を選抜し、それらのウシを用いて、暑熱ストレス耐性の遺伝的背景や暑熱耐性の分子メカニズムの解明が進めば、世界的に期待されている「乳量が多くかつ暑熱ストレス耐性の高い乳用牛」への改良に資する基盤的知見を得ることが可能になる。

Research Products

• [Int'l Joint Research] 王立農業大学/農林水産省動物衛生生産科学研究所(カンボジア)
• [Int'l Joint Research] かせサート大学(タイ)
• [Int'l Joint Research] ふぃリピン大学ロスバニョス校/国立農業水産天然資源研究開発会議(フィリピン)
• [Int'l Joint Research] 王立農業大学/農林水産省動物衛生生産科学研究所(カンボジア)
• [Int'l Joint Research] カセサート大学(タイ)
• [Int'l Joint Research] フィリピン大学ロスバニョス校/国立農業水産天然資源研究開発会議(フィリピン)
• [Int'l Joint Research] 王立農業大学/国立動物衛生生産科学研究所(カンボジア)
• [Int'l Joint Research] カセサート大学(タイ)
• [Int'l Joint Research] フィリピン大学ロスバニョス校/国立農業水産天然資源研究開発会議(フィリピン)
• [Int'l Joint Research] 王立農業大学/国立動物衛生生産科学研究所(カンボジア)
• [Int'l Joint Research] カセサート大学(タイ)
• [Int'l Joint Research] フィリピン大学ロスバニョス校/国立農業水産天然資源研究開発会議(フィリピン)
• [Journal Article] Neurokinin 3 receptor?selective agonist, senktide, decreases core temperature in Japanese Black cattle (2021)
  • Author(s): Nakamura S.、Miwa M.、Morita Y.、Ohkura S.、Yamamura T.、Wakabayashi Y.、Matsuyama S.
  • Journal Title: Domestic Animal Endocrinology Volume: 74 Pages: 106522-106522
  • DOI: 10.1016/j.domaniend.2020.106522
  • Peer Reviewed
• [Journal Article] Establishment of long-term chronic recording technique of <i>in vivo</i> ovarian parenchymal temperature in Japanese Black cows (2020)
  • Author(s): Morita Y, Ozaki R, Mukaiyama A, Sasaki T, Tatebayashi R, Morishima A, Kitagawa Y, Suzumura R, Abe R, Tsukamura H, Matsuyama S, Ohkura S.
  • Journal Title: Journal of Reproduction and Development Volume: 66 Issue: 3 Pages: 271-275
  • DOI: 10.1262/jrd.2019-097
  • NAID: 130007853975
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Seasonal changes in the reproductive performance in local cows receiving artificial insemination in the Pursat province of Cambodia (2020)
  • Author(s): Tep, B., Morita, Y., Matsuyama, S., Ohkura, S., Inoue, N., Tsukamura, H., Uenoyama, Y. and Pheng, V.
  • Journal Title: Asian-Australasian Journal of Animal Sciences Volume: 33 Issue: 12 Pages: 1922-1929
  • DOI: 10.5713/ajas.19.0893
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Peripheral administration of SB223412, a selective neurokinin-3 receptor antagonist, suppresses pulsatile luteinizing hormone secretion by acting on the gonadotropin-releasing hormone pulse generator in estrogen-treated ovariectomized female goats. (2020)
  • Author(s): Sasaki T, Sonoda T, Tatebayashi R, Kitagawa Y, Oishi S, Yamamoto K, Fujii N, Inoue N, Uenoyama Y, Tsukamura H, Maeda KI, Matsuda F, Morita Y, Matsuyama S, Ohkura S.
  • Journal Title: J Reprod Dev Volume: - Issue: 4 Pages: 351-357
  • DOI: 10.1262/jrd.2019-145
  • NAID: 130007888728
  • Peer Reviewed / Open Access
• [Journal Article] Peripheral administration of κ-opioid receptor antagonist stimulates gonadotropin-releasing hormone pulse generator activity in ovariectomized, estrogen-treated female goats (2019)
  • Author(s): Sasaki T.、Ito D.、Sonoda T.、Morita Y.、Wakabayashi Y.、Yamamura T.、Okamura H.、Oishi S.、Noguchi T.、Fujii N.、Uenoyama Y.、Tsukamura H.、Maeda K.I.、Matsuda F.、Ohkura S.
  • Journal Title: Domestic Animal Endocrinology Volume: 68 Pages: 83-91
  • DOI: 10.1016/j.domaniend.2018.12.011
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of heat stress response in crossbred dairy cows under tropical climate by analysis of heart rate variability (2018)
  • Author(s): Bun, C., Watanabe, Y., Uenoyama, Y., Inoue, N., Ieda, N., Matsuda, F., Tsukamura, H., Kuwahara, M., Maeda, K.-I., Ohkura, S. and Pheng, V.
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 1 Pages: 181-185
  • DOI: 10.1292/jvms.17-0368
  • NAID: 130006331058
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 暑熱ストレス下におけるウシの排卵不全メカニズムの解明. (2020)
  • Author(s): 向山晃永，舘林亮輝，北川悠梨，阿部良哉，鈴村玲香，松山秀一，大蔵 聡，森田康広
  • Organizer: 第113回日本繁殖生物学会大会
• [Presentation] 熱帯地域におけるウシの繁殖性向上を目指して：カンボジアを例に (2020)
  • Author(s): 森田康広，Tep, B.，Pheng, V. ，上野山賀久，大蔵 聡
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] Reproductive performance of local cows received artificial insemination in Cambodia: Suppression of calving rate during the hot/dry and wet seasons (2019)
  • Author(s): Tep, B., Morita, Y., Matsuyama, S., Ohkura, S., Inoue, N., Tsukamura, H., Uenoyama, Y. and Pheng, V.
  • Organizer: 2nd International Conference on Tropical Animal Science and Production (TASP2019)
  • Int'l Joint Research
• [Presentation] 生殖内分泌の基礎 －GnRH分泌制御の神経内分泌メカニズム－ (2019)
  • Author(s): 大蔵 聡
  • Organizer: 第1 回日本生殖発生毒性フォーラム
  • Invited
• [Presentation] Neuroendocrine control of reproductive functions in domestic ruminants (2019)
  • Author(s): Ohkura, S.
  • Organizer: The 2nd International Conference on Mathematics and Natural Sciences (IConMNS) 2019
  • Int'l Joint Research / Invited
• [Presentation] ニューロキニンB受容体作動薬投が夏季のウシ体温に及ぼす影響 (2019)
  • Author(s): 中村 翔，三輪雅史，森田康広，山村 崇，若林嘉浩，大蔵 聡，松山秀一
  • Organizer: 第112回日本繁殖生物学会大会
• [Presentation] 家畜における繁殖の神経内分泌学－基礎的知見と臨床応用の可能性－ (2019)
  • Author(s): 松田二子，大蔵 聡
  • Organizer: （公社）日本畜産学会主催日本家畜臨床学会共催公開シンポジウム「畜産研究の成果を獣医臨床フィールドへ」
  • Invited
• [Presentation] 夏季におけるウシの排卵不全の原因探索 (2019)
  • Author(s): 向山晃永，尾崎理穂，舘林亮輝，北川悠梨，阿部良哉，鈴村玲香，松山秀一，大蔵 聡，森田康広
  • Organizer: 第40回動物臨床医学会記念年次大会
• [Presentation] 私が出会った生殖神経内分泌学－農学分野における基礎研究の意義－」 (2018)
  • Author(s): 大蔵 聡
  • Organizer: 第43回日本比較内分泌学会及びシンポジウム
  • Invited
• [Presentation] 黒毛和種牛における暑熱環境下での卵胞動態の解析 (2018)
  • Author(s): 向山晃永1、佐々木拓弥、尾崎理穂、舘林亮輝、北川悠梨、森島 愛、阿部良哉、鈴村玲香、松山秀一、大蔵 聡、森田康広
  • Organizer: 平成30年度東海畜産学会大会
• [Presentation] Evaluation of heat stress responses by analysis of heart rate variability in crossbred dairy cows under the tropical climate. (2017)
  • Author(s): Bun, C., Watanabe, Y., Ohkura, S., Uenoyama, Y., Inoue, N., Ieda, N., Matsuda, F., Tsukamura, H., Kuwahara, M., Maeda, K.-I. and Pheng, V.
  • Organizer: The 4th World Congress on Reproductive Biology
  • Int'l Joint Research
• [Presentation] Evaluation of heat stress responses by analysis of heart rate variability in dairy cows under the tropical climate. (2016)
  • Author(s): Bun, C., Ohkura, S., Uenoyama, Y., Inoue, N., Ieda, N., Matsuda, F., Tsukamura, H., Kuwahara, M., Maeda, K.-I. and Pheng, V.
  • Organizer: 1st International Conference on Tropical Animal Science and Production
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2016-07-26
  • Int'l Joint Research
• [Book] 視床下部および下垂体ホルモン（「繁殖生物学 改訂版」） (2020)
  • Author(s): 大蔵 聡
  • Total Pages: 351
  • Publisher: インターズー
  • ISBN: 9784866711102
• [Remarks] 名古屋大学大学院生命農学研究科 動物生産科学研究室
  • URL: http://www.agr.nagoya-u.ac.jp/~laps/index-j.html
• [Remarks] 名古屋大学大学院生命農学研究科 動物生産科学第1研究分野
  • URL: http://www.agr.nagoya-u.ac.jp/~laps/index-j.html