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Stoichiometric-coupled dynamics of carbon, nitrogen and phosphorus influences aerobic methane production in lake ecosystems

Research Project

Project/Area Number 16H02935
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionUniversity of Yamanashi

Principal Investigator

IWATA Tomoya  山梨大学, 大学院総合研究部, 教授 (50362075)

Co-Investigator(Kenkyū-buntansha) 篠原 隆一郎  国立研究開発法人国立環境研究所, 地域環境研究センター, 主任研究員 (00610817)
小島 久弥  北海道大学, 低温科学研究所, 助教 (70400009)
田中 健太  筑波大学, 生命環境系, 准教授 (80512467)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords好気的メタン生成 / ホスホン酸 / C-Pリアーゼ / 元素バランス / 温室効果ガス / メタン / 湖沼 / NMR / 微生物群集 / メタゲノム / トランスクリプトーム
Outline of Final Research Achievements

The present study surveyed nine Japanese deep freshwater lakes to show the pattern and mechanisms of aerobic CH4 production and subsurface methane maximum (SMM) formation. The results showed that the availability of organic carbon controls N accumulation in lake waters thereby influences the CH4 production process. The microbial community analyses revealed that the distribution of picocyanobacteria (i.e., Synechococcus) was closely related to the vertical distribution of dissolved CH4 and SMM formation. Moreover, a cross-lake comparison showed that lakes with a more abundant Synechococcus population exhibited a greater development of the SMM, suggesting that these microorganisms are the most likely cause of methane production. We conclude that the stoichiometric balance between DOC and DIN might cause the cascading responses of biogeochemical processes, from N depletion to picocyanobacterial domination, and subsequently influence SMM formation in lake ecosystems.

Academic Significance and Societal Importance of the Research Achievements

本研究成果から、温度上昇によって湖水の成層構造が変化したり、集水域の人為撹乱が増大すれば、生元素の量的バランスの変化を介して湖からの温室効果ガス放出量にまで影響が及ぶ可能性があると考えられる。とくに、流域からの有機物負荷や温度上昇による成層構造の強化により湖の表層環境において窒素やリンが枯渇すると、ピコシアノバクテリアが優占することでメタンの生成量が増加する可能性があるだろう。湖は主要なメタンの自然放出源の1つであることから、温室効果ガスの動態や気候変動の機構解明の観点からも、本研究で明らかにした湖のメタン生成プロセスは学術的・社会的意義が大きいと考えられる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (18 results)

All 2020 2019 2018 2017 2016 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (3 results)

  • [Journal Article] Linking Stoichiometric Organic Carbon?Nitrogen Relationships to planktonic Cyanobacteria and Subsurface Methane Maximum in Deep Freshwater Lakes2020

    • Author(s)
      Khatun Santona、Iwata Tomoya、Kojima Hisaya、Ikarashi Yoshiki、Yamanami Kana、Imazawa Daichi、Kenta Tanaka、Shinohara Ryuichiro、Saito Hiromi
    • Journal Title

      Water

      Volume: 12 Issue: 2 Pages: 402-402

    • DOI

      10.3390/w12020402

    • NAID

      120007166906

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aerobic methane production by planktonic microbes in lakes2019

    • Author(s)
      Khatun Santona、Iwata Tomoya、Kojima Hisaya、Fukui Manabu、Aoki Takuya、Mochizuki Seito、Naito Azusa、Kobayashi Ai、Uzawa Ryo
    • Journal Title

      Science of The Total Environment

      Volume: 696 Pages: 133916-133916

    • DOI

      10.1016/j.scitotenv.2019.133916

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of 2-aminoethylphosphonic acid in suspended particles in an ultraoligotrophic lake: a two-dimensional nuclear magnetic resonance (2D-NMR) study2018

    • Author(s)
      Shinohara Ryuichiro、Iwata Tomoya、Ikarashi Yoshiki、Sano Tomoharu
    • Journal Title

      Environmental Science and Pollution Research

      Volume: 25 Issue: 30 Pages: 30739-30743

    • DOI

      10.1007/s11356-018-1744-5

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Relationships between the composition of bacterioplankton communities and subsurface methane maximum in lakes2019

    • Author(s)
      Khatun Santona, Kojima Hisaya, Ikarashi Yoshiki, Yamanami Kana, Tanaka Kenta, Shinohara Ryuichiro, Iwata Tomoya
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 淡水生態系における好気的メタン生成プロセス2019

    • Author(s)
      岩田智也, Khatun Santona, 小島久弥, 福井学, 寺島美亜, 高須賀太一, 田中健太, 篠原隆一郎
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Community structure of planktonic bacteria relating to subsurface methane maximum of aerobic lake waters2018

    • Author(s)
      Khatun Santona, Kojima Hisaya, Iwata Tomoya, Ikarashi Yoshiki, Yamanami Kana, Tanaka Kenta, Shinohara Ryuichiro
    • Organizer
      JPGU2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of phosphorus compounds by using 2D (1 H 31 P) nuclear magnetic resonance (NMR) spectroscopy in an oligotrophic environment2017

    • Author(s)
      Shinohara R., Iwata T., Ikarashi Y., Sano T., Kohzu A.
    • Organizer
      ASLO 2017 Aquatic Sciences Meeting
    • Place of Presentation
      アメリカ合衆国、ハワイ州、ホノルル
    • Year and Date
      2017-02-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel methane production pathway in freshwater ecosystems2017

    • Author(s)
      Khatun Santona, Hisaya Kojima, Tomoya Iwata
    • Organizer
      日本地球惑星科学連合2017年大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Aerobic methane production by planktonic microbes in freshwater ecosystems2017

    • Author(s)
      Khatun Santona, Iwata Tomoya, Kojima Hisaya
    • Organizer
      第三回山岳科学学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 湖の好気的メタン極大形成に影響を及ぼす環境要因の解明2017

    • Author(s)
      山南果奈・岩田智也
    • Organizer
      第三回山岳科学学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Aerobic methane production by planktonic microbes in freshwater ecosystems2017

    • Author(s)
      Khatun Santona, Iwata Tomoya, Kojima Hisaya
    • Organizer
      2017年度生物地球化学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 湖の好気的メタン極大形成に影響を及ぼす環境要因の解明2017

    • Author(s)
      山南果奈・岩田智也
    • Organizer
      2017年度生物地球化学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 湖沼の好気環境に出現するメタン極大の形成プロセス2017

    • Author(s)
      岩田智也・小林あい・内藤あずさ・小島久弥
    • Organizer
      2017年度生物地球化学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] A novel aerobic methane production pathway in freshwater ecosystems2016

    • Author(s)
      Santona Khatun, Hisaya Kojima, and Tomoya Iwata
    • Organizer
      日本陸水学会第81回大会
    • Place of Presentation
      琉球大学(沖縄県中頭郡西原町)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 淡水生態系における新たな好気的メタン生成経路2016

    • Author(s)
      KHATUN Santona・小島久弥・岩田智也
    • Organizer
      2016年度生物地球化学研究会
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究者ホームページ

    • URL

      http://www.ccn.yamanashi.ac.jp/~tiwata/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 研究室Webページ

    • URL

      http://www.ccn.yamanashi.ac.jp/~tiwata/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究代表者ホームページ

    • URL

      http://www.js.yamanashi.ac.jp/~iwata/

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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