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Study on the novel radioprotection mechanisms in radiotolerant tardigrades

Research Project

Project/Area Number 16H02951
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation and chemicals
Research InstitutionThe University of Tokyo

Principal Investigator

Kunieda Takekazu  東京大学, 大学院理学系研究科(理学部), 准教授 (10463879)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords放射線 / クマムシ / DNA / 活性酸素種 / DNA防護 / 酸化ストレス / ゲノム編集 / 耐性 / 放射線耐性 / クロマチン構造 / ゲノム / 細胞周期
Outline of Final Research Achievements

Dsup is the protein unique to radiotolerant tardigrades which suppress DNA damage and increase radiotolerance in human cultured cells. This study indicates that Dsup has an ability to protect DNA from reactive oxygen species (ROS) and thus the radioprotective ability of Dsup is suggested to be due to suppression of indirect effects of radiation. In vitro study clearly demonstrated that Dsup’s radioprotective ability does not depend on the facilitation of DNA repair. The binding mode of Dsup to DNA molecule and the action of Dsup on reconstructed nucleosomes are well consistent with the DNA protection activity of Dsup. The study revealed novel DNA protection mechanism which may support high radiotolerance in tardigrades.

Academic Significance and Societal Importance of the Research Achievements

本研究は地球上の動物の中でも抜きん出て放射線耐性の高いクマムシについて、その独自の放射線耐性タンパク質Dsupの性状・機能を解析することで、これまで知られていなかった新たなDNA保護機構/放射線耐性機構を明らかにしたもので、放射線による生物影響の低減技術の開発につながることが期待される。

Report

(4 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (52 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (37 results) (of which Int'l Joint Research: 12 results,  Invited: 17 results) Remarks (2 results)

  • [Int'l Joint Research] Medical College of Wisconsin(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Vienna Bio Center(Austria)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Durham University(United Kingdom)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 最強とも呼ばれる可愛らしい動物・クマムシ2018

    • Author(s)
      國枝武和
    • Journal Title

      生物工学会誌

      Volume: 96 Pages: 92-95

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Comparative genomics of the tardigrades Hypsibius dujardini and Ramazzottius varieornatus2017

    • Author(s)
      Yoshida Yuki、Koutsovoulos Georgios、Laetsch Dominik R.、Stevens Lewis、Kumar Sujai、Horikawa Daiki D.、Ishino Kyoko、Komine Shiori、Kunieda Takekazu、Tomita Masaru、Blaxter Mark、Arakawa Kazuharu
    • Journal Title

      PLOS Biology

      Volume: 15 Issue: 7 Pages: e2002266-e2002266

    • DOI

      10.1371/journal.pbio.2002266

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] DNA Protection Protein, a Novel Mechanism of Radiation Tolerance: Lessons from Tardigrades2017

    • Author(s)
      Hashimoto Takuma、Kunieda Takekazu
    • Journal Title

      Life

      Volume: 7 Issue: 2 Pages: 26-26

    • DOI

      10.3390/life7020026

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 極限環境耐性を持つクマムシのゲノム戦略を読み解く2017

    • Author(s)
      橋本拓磨、國枝武和
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 75 Pages: 131-133

    • Related Report
      2017 Annual Research Report
  • [Journal Article] X線からヒト培養細胞を守るクマムシタンパク質Dsup2017

    • Author(s)
      橋本拓磨、國枝武和
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 75 Pages: 102-103

    • Related Report
      2017 Annual Research Report
  • [Journal Article] クマムシのゲノムから発見した新規タンパク質Dsupはヒト培養細胞の放射線耐性を向上させる2017

    • Author(s)
      橋本拓磨、國枝武和
    • Journal Title

      実験医学

      Volume: 35 Pages: 445-448

    • Related Report
      2017 Annual Research Report
  • [Journal Article] クマムシ固有の新規タンパク質によるヒト培養細胞の放射線耐性の向上2017

    • Author(s)
      橋本拓磨、堀川大樹、國枝武和
    • Journal Title

      放射線生物研究

      Volume: 52 Pages: 1-13

    • NAID

      40021183602

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel protein in tardigrade, Ramazzottius varieornatus, improves radio-tolerance of human cultured cells.2017

    • Author(s)
      Hashimoto Takuma、Horikawa Daiki D.、Kunieda Takekazu
    • Journal Title

      Radiation Biology Research Communications

      Volume: 52 Pages: 1-13

    • NAID

      40021183602

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.2016

    • Author(s)
      Hashimoto T, Horikawa DD, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, Toyoda A and Kunieda T
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 12808-12808

    • DOI

      10.1038/ncomms12808

    • NAID

      120005971035

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of an isogenic strain of the desiccation-sensitive tardigrade Isohypsibius myrops (Parachela, Eutardigrada) and its life history traits.2016

    • Author(s)
      Ito M, Saigo T, Abe W, Kubo T and Kunieda T
    • Journal Title

      Zool J Linn Soc

      Volume: 178(4) Issue: 4 Pages: 863-870

    • DOI

      10.1111/zoj.12449

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Tricks enabling extremotolerance of tardigrades2019

    • Author(s)
      Kunieda T
    • Organizer
      MFPL international seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 極限環境に耐える動物クマムシの耐性戦略2019

    • Author(s)
      國枝 武和
    • Organizer
      第38回分子病理研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 生体分子のストレスからのプロテクションと生命活動を如何にして両立させるか2019

    • Author(s)
      國枝武和、柴原礼良
    • Organizer
      第4回クマムシ学研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クマムシ固有の細胞外分泌性脂肪酸結合タンパク質SAHSの乾燥耐性に寄与する分子メカニズムの解析2019

    • Author(s)
      田中彬寛、國枝武和
    • Organizer
      第4回クマムシ学研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 宇宙にも耐える!極限環境に耐える動物クマムシのサバイバル戦略を読み解く2019

    • Author(s)
      國枝 武和
    • Organizer
      第635回高崎研オープンセミナー
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 極限環境に耐える動物クマムシから切り拓く新たな耐性メカニズム2019

    • Author(s)
      國枝 武和
    • Organizer
      第2回理研科学者会議セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 脱水ストレス依存的に繊維/液滴状に凝集するクマムシタンパク質の解析2019

    • Author(s)
      田中彬寛、中野智美、西郷永希子、安井玲太朗、秦裕子、尾山大明、國枝武和
    • Organizer
      極限環境生物学会2019年度(第20回)年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 極限環境耐性動物クマムシにおけるゲノム編集2019

    • Author(s)
      熊谷瞳、國枝武和
    • Organizer
      第42回 日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脱水ストレスに応答して繊維/液滴状構造を形成するクマムシタンパク質群の同定と解析2019

    • Author(s)
      田中彬寛、中野智美、西郷永希子、安井玲太朗、秦裕子、尾山大明、國枝武和
    • Organizer
      第42回 日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The rise of tardigrade-unique toolbox for extremotolerance2018

    • Author(s)
      Kunieda T
    • Organizer
      14th International Symposium on Tardigrada
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Tolerance to gamma radiation in the desiccation-sensitive tardigrade Isohypsibius myrops (Parachela, Eutardigrada)2018

    • Author(s)
      Saigo T, Ito M, Satoh K, Kubo T and Kunieda T
    • Organizer
      14th International Symposium on Tardigrada
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] AFM analysis of DNA binding mode of a novel DNA-protection protein, Dsup, unique to a radiotolerant tardigrade2018

    • Author(s)
      Shibahara R, Hizume K, Hashimoto T, Araki H, Kubo T and Knieda T
    • Organizer
      14th International Symposium on Tardigrada
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 最強動物!?クマムシのサバイバル戦略を読み解く2018

    • Author(s)
      國枝 武和
    • Organizer
      動物学会関東支部公開講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 極限環境に耐えるクマムシの耐性戦略に迫る2018

    • Author(s)
      國枝 武和
    • Organizer
      第一回再生学異分野融合研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 極限環境に耐えるクマムシの耐性戦略を読み解く2018

    • Author(s)
      國枝武和
    • Organizer
      第78回日本寄生虫学会東日本支部大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 高い放射線耐性を持つクマムシに固有なDNA防護タンパク質DsupのDNA保護メカニズムの解析2018

    • Author(s)
      柴原 礼良、日詰 光治、橋本 拓磨、荒木 弘之、國枝 武和
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クマムシ固有の分泌型熱可溶性タンパク質SAHSの翻訳後修飾の解析2018

    • Author(s)
      田中彬寛、山口志保、國枝武和
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 乾燥耐性をもたないクマムシIsohypsibius myropsの放射線耐性2018

    • Author(s)
      西郷 永希子、伊藤 麻紀子、佐藤 勝也、國枝 武和
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クマムシ固有の分泌型熱可溶性タンパク質SAHSの翻訳後修飾の解析2018

    • Author(s)
      田中彬寛、山口志保、國枝武和
    • Organizer
      第19回極限環境生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 乾燥耐性をもたないクマムシIsohypsibius myropsの放射線耐性2018

    • Author(s)
      西郷 永希子、伊藤 麻紀子、佐藤 勝也、國枝 武和
    • Organizer
      第19回極限環境生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Decoding the Molecular Strategy of Radiotolerance in Extremotolerant Animal Model, Tardigrades2018

    • Author(s)
      Kunieda T
    • Organizer
      ENDO2018, the Endocrine Society’s 100th annual meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Decoding the Molecular Strategy of Radiotolerance in Extremotolerant Animal Model, Tardigrades2018

    • Author(s)
      Kunieda T
    • Organizer
      the Endocrine Society’s 100th annual meeting
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Unique strategy for amazing resilience of tardigrades2018

    • Author(s)
      Kunieda T
    • Organizer
      Organs under stress symposium
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Decoding of unique strategy for extreme tolerance in tardigrades2017

    • Author(s)
      Kunieda T
    • Organizer
      Vienna Biocenter Seminar
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ベールを脱ぎ始めたクマムシの極限環境耐性メカニズム2017

    • Author(s)
      國枝武和、橋本拓磨、堀川大樹、近藤小雪、田中冴、柴原礼良、桑原宏和、秦裕子、尾山大明、榎本敦、宮川清、原雄一郎、横堀伸一、小原雄治、藤山秋佐夫、久保健雄、荒川和晴、片山俊明、豊田敦
    • Organizer
      2017年度生命科学系学会合同年次大会ConBio2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 動物の極限環境耐性から生命の本質を探る2017

    • Author(s)
      國枝武和
    • Organizer
      未来へのバイオ技術勉強会-超絶生物イノベーション
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Invited
  • [Presentation] 高い放射線耐性を持つクマムシに固有なDNA防護タンパク質DsupのDNA結合様式の解析2017

    • Author(s)
      柴原礼良、日詰光治、橋本拓磨、荒木弘之、久保健雄、國枝武和
    • Organizer
      2017年度生命科学系学会合同年次大会ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高い放射線耐性を持つクマムシに固有なDNA防護タンパク質DsupのDNA結合様式の解析2017

    • Author(s)
      柴原礼良、日詰光治、橋本拓磨、久保健雄、國枝武和
    • Organizer
      極限環境生物学会2017年度年会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Novel tardigrade protein protects DNA from radiation and ROS in human cultured cells2017

    • Author(s)
      Kunieda T
    • Organizer
      International Symposium on LIVING IN SPACE 2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヨコヅナクマムシ由来細胞の分離法の確立と耐性能力の解析2017

    • Author(s)
      小島広樹、久保健雄、國枝武和
    • Organizer
      日本動物学会関東支部第69回大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ベールを脱ぎ始めたクマムシの極限環境耐性メカニズム2017

    • Author(s)
      國枝武和、橋本拓磨、堀川大樹、近藤小雪、田中冴、柴原礼良、桑原宏和、秦裕子、尾山大明、榎本敦、宮川清、原雄一郎、横堀伸一、小原雄治、藤山秋佐夫、久保健雄、荒川和晴、片山俊明、豊田敦
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 高い放射線耐性を持つクマムシに固有なDNA防護タンパク質DsupのDNA結合様式の解析2017

    • Author(s)
      柴原礼良、日詰光治、橋本拓磨、荒木弘之、久保健雄、國枝武和
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 極限環境に耐える動物クマムシの秘密を探る2016

    • Author(s)
      國枝武和
    • Organizer
      第72回日本寄生虫学会西日本支部大会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 放射線耐性動物クマムシの新規タンパク質によるヒト培養細胞におけるDNA傷害の抑制と放射線耐性の向上2016

    • Author(s)
      橋本拓磨、斎藤裕樹、秦裕子、榎本敦、堀川大樹、荒川和晴、片山俊明、豊田敦、尾山大明、宮川清、久保健雄、國枝武和
    • Organizer
      日本放射線影響学会第59回大会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 極限環境に耐えるヨコヅナクマムシのゲノム戦略2016

    • Author(s)
      國枝武和、橋本拓磨、堀川大樹、桑原宏和、近藤小雪、田中冴、原雄一郎、横堀伸一、小原雄治、藤山秋佐夫、久保健雄、荒川和晴、片山俊明、豊田敦
    • Organizer
      第17回極限環境生物学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Correct Decoding of Genomic Strategy in Extremotolerant Tardigrade, Ramazzottius varieornatus2016

    • Author(s)
      Kunieda T, Hashimoto T, Horikawa DD, Kuwahara H, Kondo K, Tanaka S, Saigo T, Kubo T, Fujiyama A, Arakawa K, Katayama T, Toyoda A
    • Organizer
      11th International Congress on Extremophiles
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of PP1/PP2A in Tolerance and the Initial Transcriptional Response to Desiccation in Anhydrobiotic Tardigrade, Hypsibius dujardini2016

    • Author(s)
      Kondo K, Katou Y, Shirahige K, Kubo T, Kunieda T
    • Organizer
      11th International Congress on Extremophiles
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Remarks] くまむし研究グループ

    • URL

      http://www.bs.s.u-tokyo.ac.jp/~saibou/kuma/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] ヒト培養細胞の放射線耐性を向上させる新規タンパク質をクマムシのゲノムから発見

    • URL

      https://www.s.u-tokyo.ac.jp/ja/press/2016/5001/

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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