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Analysis of transcription-coupled double strand break repair

Research Project

Project/Area Number 16H02954
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation and chemicals
Research InstitutionFukuoka University (2017-2019)
Osaka University (2016)

Principal Investigator

Kuraoka Isao  福岡大学, 理学部, 教授 (60335396)

Co-Investigator(Kenkyū-buntansha) 塩井 成留実 (青木成留実)  福岡大学, 理学部, 助教 (50510187)
竹立 新人  福岡大学, 理学部, 助教 (20846505)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
KeywordsDNA修復 / 転写 / DNA鎖切断 / 環境 / 放射線 / DNA損傷 / 修復 / 転写産物 / 影響評価 / ゲノム修復
Outline of Final Research Achievements

Ionizing radiation directly affects DNA structure by inducing DNA breaks, particularly, DSBs. Secondary effects are the generation of reactive oxygen species that oxidize proteins and lipids, and also induce several damages to DNA. Oxidative DNA damage induces genomic instability and may lead to mutagenesis and carcinogenesis. These DNA lesions interfere not only with replication but also with transcription. Thus, it is thought that the blockage of transcriptional machinery at the damaged DNA site of the transcribed strand triggers transcription-coupled DNA repair and recruits other DNA repair factors to repair the transcription-blocking lesions on the transcribed strand.
Here, we designed new vector construct that can be detect DNA repair pathways with transcription in vivo and found that transcription-coupled double strand break repair can be too quick to analyze the mechanism.

Academic Significance and Societal Importance of the Research Achievements

ヒト細胞において,DNA損傷に対する反応はより多種多様である。例えば,ヒト体細胞においてはほとんどが休止期の細胞,つまり複製より転写の方がより機能し,DNA損傷の影響は転写反応に現れてくると考えられる。本研究はDNA鎖切断がヒトの転写機構において,どのように影響を与え,それを回避していくのかを明らかにする実験を行った。この結果は転写における放射線細胞障害の解明に貢献していると思われる。

Report

(4 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (25 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (16 results) (of which Int'l Joint Research: 10 results,  Invited: 1 results)

  • [Int'l Joint Research] National Institutes of Health(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Barcelona(スペイン)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effects of acetaldehyde-induced DNA lesions on DNA metabolism2020

    • Author(s)
      Haruka Tsuruta, Yuina Sonohara, Kosuke Tohashi, Narumi Aoki Shioi, Shigenori Iwai and Isao Kuraoka
    • Journal Title

      Genes and Environment

      Volume: 42 Issue: 1 Pages: 1-1

    • DOI

      10.1186/s41021-019-0142-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolution of Inosine-Specific Endonuclease V from Bacterial DNase to Eukaryotic RNase.2019

    • Author(s)
      Wu J, Samara NL, Kuraoka I, Yang W.
    • Journal Title

      Mol Cell.

      Volume: 76 Issue: 1 Pages: 44-56

    • DOI

      10.1016/j.molcel.2019.06.046

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nucleotide excision repair genes shaping embryonic development.2019

    • Author(s)
      Araujo SJ, Kuraoka I.
    • Journal Title

      Open Biol.

      Volume: 9 Issue: 10 Pages: 190166-190166

    • DOI

      10.1098/rsob.190166

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An assay to detect DNA-damaging agents that induce nucleotide excision-repairable DNA lesions in living human cells2017

    • Author(s)
      Takatsuka Reine、Ito Shunsuke、Iwai Shigenori、Kuraoka Isao
    • Journal Title

      Mutation Research/Genetic Toxicology and Environmental Mutagenesis

      Volume: 820 Pages: 1-7

    • DOI

      10.1016/j.mrgentox.2017.05.009

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Facile preparation of a fluorescent probe to detect the cellular ability of nucleotide excision repair.2017

    • Author(s)
      Tawarahara H, Kuraoka I, Iwai S.
    • Journal Title

      Anal Biochem.

      Volume: 526 Pages: 71-74

    • DOI

      10.1016/j.ab.2017.03.023

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3'-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease.2016

    • Author(s)
      Yamamoto J, Takahata C, Kuraoka I, Hirota K, Iwai S.
    • Journal Title

      Molecules

      Volume: 21(6) Issue: 6 Pages: 766-766

    • DOI

      10.3390/molecules21060766

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inosine-specific ribonuclease activity of natural variants of human endonuclease V.2016

    • Author(s)
      Kim JI, Tohashi K, Iwai S, Kuraoka I.
    • Journal Title

      FEBS Lett.

      Volume: 590 Issue: 23 Pages: 4354-4360

    • DOI

      10.1002/1873-3468.12470

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] New biochemical function of EEPD1 protein2019

    • Author(s)
      Shota Ueda, Isao Kuraoka
    • Organizer
      16th International Congress of Radiation Research 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Developing towards anti-venom drugs by endogenous inhibitor against the metalloproteinase induced hemorrhage; Rational design of drug and therapeutic potential for snakebite2019

    • Author(s)
      Ayaka Ogura, Yoshifumi Zaitsu, Riku Tsutsumi, Isao Kuraoka, R Manjunatha Kini; Narumi Aoki-Shioi
    • Organizer
      The 20th World Congress of the International Society on Toxinology Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヘビ毒ホスホジエステラーゼの基質DNA配列の優先性2019

    • Author(s)
      財津 佳史、塩井 成留実、倉岡 功
    • Organizer
      第6回アジア環境変異原学会・日本環境変異原学会第48回大会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アセトアルデヒドによるヒストンテールへの影響2019

    • Author(s)
      堤陸、 四ケ所亮介, 小倉彩香, 倉岡 功, 塩井 成留実
    • Organizer
      第6回アジア環境変異原学会・日本環境変異原学会第48回大会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Funcitional analysis of Endonuclease/exonuclease/phosphatase family domain containing 1 protein2019

    • Author(s)
      Shota Ueda, Isao Kuraoka
    • Organizer
      第6回アジア環境変異原学会・日本環境変異原学会第48回大会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inosine-specific ribonuclease activity of human endonuclease V isoforms2019

    • Author(s)
      Mayu Kawasaki, Isao Kuraoka
    • Organizer
      第6回アジア環境変異原学会・日本環境変異原学会第48回大会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] T7 endonuclease 1 cleaves UV-induced DNA lesion2019

    • Author(s)
      Kazuki Matusubara, Isao Kuraoka
    • Organizer
      第6回アジア環境変異原学会・日本環境変異原学会第48回大会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Alternative excision repair model for topoisomerase mediated DNA damage2018

    • Author(s)
      Kuraoka Isao
    • Organizer
      18th All India Congress of Cytology and Genetics & International Symposium on Translating Genes and Genomes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An Assay to Detect DNA-Damaging Agents that Induce Nucleotide Excision-Repairable DNA Lesions2017

    • Author(s)
      Reine Takatsuka, Shigenori Iwai, Noriko Suematsu, Narumi Shioi and Isao Kuraoka
    • Organizer
      THE 12TH INTERNATIONAL CONFERENCE & 5TH ASIAN CONGRESS ON ENVIRONMENTAL MUTAGENS
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] New assay to detect DNA-damaging agents in water pollution using DNA enzyme functions.2017

    • Author(s)
      Noriko Suematsu, Mika Yukutake, Narumi Shioi, Isao Kuraoka
    • Organizer
      The 5th international symposium on Aqua Science and Water Resources
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A study of a novel nucleotide excision-repairable DNA lesion2017

    • Author(s)
      Kuraoka Isao
    • Organizer
      6th US-Japan DNA repair meeting
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] T7 endonuclease Iを用いたDNA損傷剤の検出法2017

    • Author(s)
      上田翔大, 末松祝福子, 行武美華, 塩井(青木)成留実, 倉岡功
    • Organizer
      日本環境変異原学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヌクレオチド切除修復システムによって修復されるDNA損傷の検出アッセイ2017

    • Author(s)
      倉岡功, 高塚玲音, 岩井成憲
    • Organizer
      日本環境変異原学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ERCC1‐XPFおよびRPAはトポイソメラーゼI‐DNA複合体の修復に関与する2016

    • Author(s)
      倉岡功
    • Organizer
      第45回日本環境変異原学会大会
    • Place of Presentation
      Tsukuba
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 構造特異的ヌクレアーゼ ERCC1-XPF はトポイソメラーゼ I-DNA 複合体の修復に関与する2016

    • Author(s)
      倉岡功、高畑千晶、岩井成憲
    • Organizer
      第59回日本放射線影響学会
    • Place of Presentation
      Hiroshima
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヌクレオチド除去修復の修復合成は、TOPOI-DNA 複合体のDNA修復に関与する。2016

    • Author(s)
      倉岡功
    • Organizer
      第75回日本癌学会
    • Place of Presentation
      Yokohama
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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