| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
We established a system to detect action-at-a-distance mutations (untargeted mutations found at positions apart from a DNA lesion) by incorporating the DNA lesion of interest at a downstream position of the supF gene (a reporter gene for mutations). An oxidatively damaged base, 8-hydroxyguanine, was examined using this system and found to induce the action-at-a-distance mutations (base substitution mutations in this case) frequently when the WRN protein was reduced.
We also examined another kind of DNA lesion, an abasic site. We found that it induced the action-at-a-distance mutations observed in the 8-hydroxyguanine experiments and a different type of action-at-a-distance mutations (large deletions).
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