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Nitrogen cycle by subterranean microbial community; Groundwater flow and microbial denitrification in deep aquifer associated with the accretionary prisms

Research Project

Project/Area Number 16H02968
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental impact assessment
Research InstitutionShizuoka University

Principal Investigator

Kimura Hiroyuki  静岡大学, グリーン科学技術研究所, 教授 (30377717)

Co-Investigator(Kenkyū-buntansha) 木庭 啓介  京都大学, 生態学研究センター, 教授 (90311745)
光延 聖  愛媛大学, 農学研究科, 准教授 (70537951)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords微生物 / 地下圏 / 脱窒 / メタン生成 / 発酵 / 物質循環 / 地下水流動 / 地殻変動 / 窒素循環
Outline of Final Research Achievements

Accretionary prisms are composed mainly of ancient marine sediment scraped from a subducting oceanic plate at a convergent plate boundary. Large amounts of anaerobic groundwater and natural gas, mainly methane (CH4) and nitrogen gas (N2), are present in the deep aquifers associated with an accretionary prism, but the origin of N2 is poorly understood. The present study demonstrated that N2 production through the anaerobic oxidation of organic matter by denitrifying bacteria is particularly prevalent in the deep aquifers of the accretionary prism. DNA analysis revealed the dominance of the genus Thauera, reported to be capable of nitrate reduction and denitrification using organic matter as electron donor under anaerobic conditions. The denitrifying bacteria may compete with the CH4-producing syntrophic consortium for organic matter in the accretionary prism sediments.

Academic Significance and Societal Importance of the Research Achievements

付加体は、西南日本の太平洋側の地域に広く分布する厚い堆積層である。本研究課題では、付加体の深部帯水層における炭素循環および窒素循環についての新たな知見を数多く得ることができた。そして、地の利を活かした日本発の特色ある研究を推進できた。また、地殻変動が頻繁に発生する付加体の深部帯水層での炭素循環に関する知見が得られたことから、温暖化メカニズムの解明にも貢献した。さらに、本研究課題において深部地下圏での微生物による脱窒プロセスや炭素循環、窒素循環を総合的に理解したことにより、新エネルギーの開発に必要な基盤情報の提供に繋がった。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (46 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 10 results,  Acknowledgement Compliant: 3 results) Presentation (24 results) (of which Int'l Joint Research: 4 results,  Invited: 6 results) Book (3 results) Remarks (3 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] Department of Oceanography/University of Hawaii at Manoa(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Hawaii, Manoa(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] The Potential for CH<sub>4</sub> Production by Syntrophic Microbial Communities in Diverse Deep Aquifers Associated with an Accretionary Prism and its Overlying Sedimentary Layers2020

    • Author(s)
      Matsushita Makoto、Ishikawa Shugo、Magara Kenta、Sato Yu、Kimura Hiroyuki
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME19103

    • NAID

      130007784459

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional diversification of two bilin reductases for light perception and harvesting in unique cyanobacterium Acaryochloris marina MBIC 110172020

    • Author(s)
      Miyake Keita、Fushimi Keiji、Kashimoto Tomonori、Maeda Kaisei、Ni‐Ni‐Win、Kimura Hiroyuki、Sugishima Masakazu、Ikeuchi Masahiko、Narikawa Rei
    • Journal Title

      The FEBS Journal

      Volume: - Issue: 18 Pages: 4016-4031

    • DOI

      10.1111/febs.15230

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Filtration and exposure to benzalkonium chloride or sodium chloride to preserve water samples for dissolved inorganic carbon analysis2019

    • Author(s)
      Takahashi Hiroshi A.、Handa Hiroko、Sugiyama Ayumi、Matsushita Makoto、Kondo Miyuki、Kimura Hiroyuki、Tsujimura Maki
    • Journal Title

      GEOCHEMICAL JOURNAL

      Volume: 53 Issue: 5 Pages: 305-318

    • DOI

      10.2343/geochemj.2.0570

    • NAID

      130007724555

    • ISSN
      0016-7002, 1880-5973
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 付加体深部帯水層での微生物メタン生成とエネルギー生産システムの社会実装2019

    • Author(s)
      木村浩之、松下 慎、芦沼完太、津布久卓也
    • Journal Title

      日本微生物生態学会誌

      Volume: 34 Pages: 43-48

    • NAID

      130007716349

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Geochemical and Microbiological Evidence for Microbial Methane Production in Deep Aquifers of the Cretaceous Accretionary Prism2018

    • Author(s)
      Makoto Matsushita, Kenta Magara, Yu Sato, Naoya Shinzato, Hiroyuki Kimura
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 2 Pages: 205-213

    • DOI

      10.1264/jsme2.ME17199

    • NAID

      130007393738

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Temperature-dependent expression of different guanine-plus-cytosine content 16S rRNA genes in Haloarcula strains of the class Halobacteria2018

    • Author(s)
      Sato Yu、Kimura Hiroyuki
    • Journal Title

      Antonie van Leeuwenhoek

      Volume: 112 Issue: 2 Pages: 187-201

    • DOI

      10.1007/s10482-018-1144-3

    • NAID

      120006502988

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Geochemical and microbiological evidence for microbial methane production in deep aquifers of the Cretaceous accretionry prism2018

    • Author(s)
      Makoto Matsushita, Kenta Magara, Yu Sato, Naoya Shinzato, Hiroyuki Kimura
    • Journal Title

      Microbes and Environments

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea2017

    • Author(s)
      T. Kono, S. Mehrotra, C. Endo, N. Kizu, M. Matsuda, H. Kimura, E. Mizohata, T. Inoue, T. Hasunuma, A. Yokota, H. Matsumura, H. Ashida
    • Journal Title

      Nature Commun.

      Volume: 8 Issue: 1 Pages: 14007-14007

    • DOI

      10.1038/ncomms14007

    • NAID

      120005947453

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Expression and function of different guanine-plus-cytosine content 16S rRNA genes in Haloarcula hispanica at different temperatures2017

    • Author(s)
      Yu Sato, Taketomo Fujiwara, Hiroyuki Kimura
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 482-482

    • DOI

      10.3389/fmicb.2017.00482

    • NAID

      120006312524

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regional Variation of CH<sub>4</sub> and N<sub>2</sub> Production Processes in the Deep Aquifers of an Accretionary Prism2016

    • Author(s)
      Makoto Matsushita, Shugo Ishikawa, Kazushige Nagai, Yuichiro Hirata, Kunio Ozawa, Satoshi Mitsunobu, Hiroyuki Kimura
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 3 Pages: 329-338

    • DOI

      10.1264/jsme2.ME16091

    • NAID

      130005265117

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 微生物により生成されるメタンの濃度測定へのレーザー吸収分光法の適用2019

    • Author(s)
      川野 誠、中山洋平、木村浩之
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 地下帯水層に生息する新規微生物群集の遺伝子解析2019

    • Author(s)
      内野正洋、木村浩之
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 嫌気性地下水に含まれる水素発生型発酵細菌を利用した水素ガス生成システムの構築2019

    • Author(s)
      津布久卓也、木村浩之
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 地下圏の脱窒とメタン生成の有機物利用における競合:菌叢の違いは関係ない?2019

    • Author(s)
      芦沼完太、松下 慎、木村浩之
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 温泉付随メタンガス発電施設の調査と防災ステーションへの展開2019

    • Author(s)
      石垣治久、木村浩之、天野幸治
    • Organizer
      2019年度自然災害科学中部地区研究集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Function of different guanine-plus-cytosine content 16S rRNAs in halophilic archaea at various temperatures2018

    • Author(s)
      Hiroyuki Kimura
    • Organizer
      5th World Congress on Microbial Biotechnology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 静岡県の地層と微生物とメタンの関係 ~基礎研究から新エネルギー生産まで~2018

    • Author(s)
      木村 浩之
    • Organizer
      日本土壌肥料学会中部支部第98回例会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 海底堆積物からの贈り物、メタン! ~分散型エネルギー生産システムの構築を目指して~2018

    • Author(s)
      木村 浩之
    • Organizer
      第11回アクセラレーション技術発表討論会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 海底堆積物からの贈り物、メタン!~地球科学と微生物生態学の融合研究からメタン・水素ガス生成リアクターの開発まで~2018

    • Author(s)
      木村 浩之
    • Organizer
      名古屋工業大学バイオ活用土木環境システム研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 地球ダイナミクスと微生物メタン生成 ~微生物生態学から新エネルギーの社会実装まで~2018

    • Author(s)
      木村 浩之
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 地下温水中の微生物群集を利用した水素ガス生成システムの開発2018

    • Author(s)
      津布久卓也、松下 慎、木村浩之
    • Organizer
      第19回静岡ライフサイエンスシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 深部帯水層の地下圏微生物を対象とした基盤研究と温泉メタンガス発電事業の推進2018

    • Author(s)
      荻祐太朗、松下 慎、木村浩之
    • Organizer
      第19回静岡ライフサイエンスシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 付加体の深部帯水層中における N2 生成メカニズムの解明2018

    • Author(s)
      芦沼完太、松下 慎、佐藤 悠、木村浩之
    • Organizer
      第19回静岡ライフサイエンスシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 川根温泉の地下帯水層におけるメタン生成プロセスと分散型エネルギー生産システム2017

    • Author(s)
      荻祐太朗、石川修伍、松下 慎、木村浩之
    • Organizer
      第18回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡大学(静岡県静岡市)
    • Year and Date
      2017-03-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] 沖縄本島の深部帯水層に生息する地下圏微生物とメタン生成2017

    • Author(s)
      眞柄健太、松下 慎、佐藤 悠、木村浩之
    • Organizer
      第18回静岡ライフサイエンスシンポジウム
    • Place of Presentation
      静岡大学
    • Year and Date
      2017-03-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Regional variation of CH4 and N2 production processes in the deep aquifers of an accretionary prism2017

    • Author(s)
      Makoto Matsushita, Hiroyuki Kimura
    • Organizer
      2017 International Symposium toward the Future of Advanced Researches in Shizuoka University
    • Place of Presentation
      静岡大学(静岡県静岡市)
    • Year and Date
      2017-02-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evidence of microbial methane generation in the deep aquifer of accretionary prism in southern Okinawa Island, Japan2017

    • Author(s)
      Kenta Magara, Makoto Matsushita, Yu Sato, Hiroyuki Kimura
    • Organizer
      2017 International Symposium toward the Future of Advanced Researches in Shizuoka University
    • Place of Presentation
      静岡大学(静岡県静岡市)
    • Year and Date
      2017-02-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Radiocarbon changes of unsterilized water samples during the long-term storage2017

    • Author(s)
      Hiroshi Takahashi, Masayo Minami, Takafumi Aramaki, Hiroyuki Kimura
    • Organizer
      The Fourteenth International AMS Conference (ASM14)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 付加体の温泉付随ガスと地下圏微生物を対象とした基盤研究による川根温泉メタンガス発電システムの創成2017

    • Author(s)
      荻 祐太朗、松下 慎、木村 浩之
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水試料の溶存無機炭素分析における殺菌処理:水銀を用いない手法の提案2017

    • Author(s)
      高橋 浩、半田宙子、松下 慎、木村浩之
    • Organizer
      日本地球化学会年会第64回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 付加体の深部帯水層におけるメタン及び窒素ガス生成プロセスの地域特性2016

    • Author(s)
      松下 慎、石川修伍、眞柄健太、光延 聖、木村浩之
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館(神奈川県横須賀市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 沖縄本島に分布する付加体の深部帯水層におけるメタン生成メカニズム2016

    • Author(s)
      眞柄健太、松下 慎、佐藤 悠、石川修伍、新里尚也、木村浩之
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館(神奈川県横須賀市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 付加体の深部帯水層におけるCH4とN2の生成プロセスの地域特性2016

    • Author(s)
      松下 慎、石川修伍、平田悠一郎、眞柄健太、木村浩之
    • Organizer
      日本地球惑星科学連合2016年大会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-05-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 沖縄本島南部に分布する付加体の深部帯水層におけるメタン生成メカニズム2016

    • Author(s)
      眞柄健太、松下 慎、石川修伍、平田悠一郎、木村浩之
    • Organizer
      日本地球惑星科学連合2016年大会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-05-22
    • Related Report
      2016 Annual Research Report
  • [Book] 静岡の大規模自然災害の科学2020

    • Author(s)
      静岡大学防災総合センター編(岩田孝仁・北村晃寿・小山真人編)
    • Total Pages
      256
    • Publisher
      静岡新聞社
    • ISBN
      9784783805557
    • Related Report
      2019 Annual Research Report
  • [Book] 駿河湾学2017

    • Author(s)
      木村浩之、松下 慎
    • Total Pages
      160
    • Publisher
      東海大学出版部
    • ISBN
      9784486021445
    • Related Report
      2017 Annual Research Report
  • [Book] 海洋底科学の基礎2016

    • Author(s)
      日本地質学会「海洋底科学の基礎」編集委員会
    • Total Pages
      389
    • Publisher
      共立出版
    • Related Report
      2016 Annual Research Report
  • [Remarks] 木村研究室ホームページ

    • URL

      http://kimura-lab.sci.shizuoka.ac.jp/top.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 静岡大学木村研究室ホームページ

    • URL

      http://kimura-lab.sci.shizuoka.ac.jp/top.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] 木村研究室ホームページ

    • URL

      http://www.ipc.shizuoka.ac.jp/~shkimur/top.html

    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] メタン生成装置2020

    • Inventor(s)
      川野 誠、木村浩之
    • Industrial Property Rights Holder
      川野 誠、木村浩之
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-052932
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 水素ガス生成方法、水素ガス生成システム、並びに、水素ガス及びメタン生成システム2019

    • Inventor(s)
      木村浩之
    • Industrial Property Rights Holder
      静岡大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2018 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 水素ガス生成方法、水素ガス生成システム、並びに、水素ガス及びメタン生成システム2018

    • Inventor(s)
      木村浩之
    • Industrial Property Rights Holder
      国立大学法人静岡大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-037195
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] バイオリアクター、それを用いたメタン生成方法及び水素ガス生成方法、並びに水/ガス/電気の自家的供給システム2017

    • Inventor(s)
      木村浩之、増田俊明
    • Industrial Property Rights Holder
      静岡大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Acquisition Date
      2018
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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