Research on degradation systems of persistent chlorinated organophosphorus triesters in the triesters-degrading bacteria.

• Project/Area Number: 16H02974
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental engineering and reduction of environmental burden
• Research Institution: Nagaoka University of Technology
• Principal Investigator: Kera Yoshio 長岡技術科学大学, 工学研究科, 教授 (00137168)
• Co-Investigator(Kenkyū-buntansha): 阿部 勝正 長岡技術科学大学, 工学研究科, 助教 (40509551) ; 高橋 祥司 長岡技術科学大学, 工学研究科, 教授 (90324011)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
• Keywords: 環境技術 / 難分解性有害物 / 微生物分解 / 分解酵素 / 遺伝子 / 遺伝子発現調節 / 酵素

Outline of Final Research Achievements

The chlorinated organophosphorus compounds tris(2-chloroethyl) phosphate (TCEP) and tris(1,3-dichloro-2-propyl)phosphate (TDCPP) are persistent and widespread contaminants in various environments. Many studies have shown several toxic effects of the compounds. We have isolated novel bacteria degrading trihaloalkyl phosphates, Sphingobium sp. strain TCM1 and Sphingomonas sp. strain TDK1.
In this study, we analyzed the degradation systems of persistent chlorinated organophosphorus triesters in the triesters-degrading bacteria, the strain TCM1.

Academic Significance and Societal Importance of the Research Achievements

我々が世界で初めて単離に成功した含塩素有機リン酸トリエステル類分解菌 Sphingobium sp. TCM1 株に存在する分解システムとその調節機構を詳細に解析することは、学術的な貢献度が極めて高く、かつ独創的な点である。
また、得られた知見は、当該化合物を含む廃水の処理や汚染水域の環境保全・修復など、世界に先駆けた環境技術開発の基礎となるものであり、社会的な意義や貢献度も極めて大きい。

Research Products

• [Journal Article] Enzymatic characterization and regulation of gene expression of PhoK alkaline phosphatase in Sphingobium sp. strain TCM1. (2020)
  • Author(s): Takahashi S, Morooka Y, Kumakura T, Abe K, Kera Y.
  • Journal Title: Appl. Microbiol. Biotechnol. Volume: 104 Issue: 3 Pages: 1125-1134
  • DOI: 10.1007/s00253-019-10291-6
  • Peer Reviewed
• [Journal Article] An atypical phosphodiesterase capable of degrading haloalkyl phosphate diesters from Sphingobium sp. strain TCM1. (2017)
  • Author(s): Abe，K., Mukai, N., Morooka, Y., Makino, T., Oshima, K., Takahashi, S. and Kera, Y.
  • Journal Title: Sci. Rep. Volume: ７ Issue: 1 Pages: 2842-2842
  • DOI: 10.1038/s41598-017-03142-9
  • Peer Reviewed / Open Access
• [Journal Article] Identification of alkaline phosphatase genes for utilizing a flame retardant, tris(2-chloroethyl) phosphate, in Sphingobium sp. strain TCM1. (2017)
  • Author(s): Takahashi, S., Katanuma, H., Abe, K., Kera, Y.
  • Journal Title: Appl.Microbiol. Biotechnol. Volume: 101 Issue: 5 Pages: 2153-2162
  • DOI: 10.1007/s00253-016-7991-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Draft genome sequences of Sphingobium sp. strain TCM1 and Sphingomonas sp. strain TDK1, haloalkyl phosphate flame retardant and plasticizer-degrading bacteria. (2016)
  • Author(s): Kera, Y., Abe, K., Kasai, D., Fukuda, M., Takahashi, S.
  • Journal Title: Genome Announcement Volume: 4 Issue: 4
  • DOI: 10.1128/genomea.00668-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 難分解性含塩素有機リン化合物を分解する新規ホスホトリエステラーゼ． (2019)
  • Author(s): 阿部勝正、高橋祥司、解良芳夫
  • Organizer: 第12回北陸合同バイオシンポジウム
• [Presentation] 換え大腸菌を用いた高効率リン酸トリス(2-クロロエチル)分解． (2019)
  • Author(s): 阿部勝正、 篠原輝、 高橋祥司、 解良芳夫
  • Organizer: 第 71 回日本生物工学会大会
• [Presentation] Investigation of the Role of Signal-like Sequence of Organophosphorus Hydrolase from Sphingobium sp. TCM1. (2018)
  • Author(s): Seiya Konno, Toshihiro Imai, Katsumasa Abe, Shouji Takahashi, Yoshio Kera
  • Organizer: The 7th international GIGAKU conference in Nagaoka (IGCN2018)
  • Int'l Joint Research
• [Presentation] Expression and Purification of Phosphodiesterase from Sphingobium sp. strain TCM1. (2018)
  • Author(s): Masayuki Kawaguchi, Takeshi Makino, Katsumasa Abe, Shouji Takahashi, Yoshio Kera
  • Organizer: The 7th international GIGAKU conference in Nagaoka (IGCN2018)
  • Int'l Joint Research
• [Presentation] ハロアルキル有機リン酸トリエステル加水分解酵素中のシグナル様ペプチドの機能解析． (2018)
  • Author(s): 今野 聖矢, 今井 俊宏, 阿部 勝正, 高橋 祥司, 解良 芳夫
  • Organizer: 第 70 回日本生物工学会大会
• [Presentation] 変異型ハロアルキル有機リン酸トリエステル加水分解酵素の大腸菌発現系の構築と諸特性解析． (2018)
  • Author(s): 阿部 勝正, 田中 成美, 高橋 祥司, 解良 芳夫
  • Organizer: 第 70 回日本生物工学会大会
• [Presentation] Sphingobium sp. TCM1株ホスホジエステラーゼの発現と精製． (2018)
  • Author(s): 川口 誠之，牧野 剛，高橋 祥司, 解良 芳夫
  • Organizer: 第91回日本生化学会大会
• [Presentation] 有機リン加水分解酵素中に見いだされた新奇シグナル様配列の機能解析． (2018)
  • Author(s): 阿部 勝正,今野 聖矢, 今井 俊宏, 高橋 祥司, 解良 芳夫
  • Organizer: 第91回日本生化学会大会
• [Presentation] 含塩素有機リン難燃剤分解菌 Sphingobium sp. TCM1 株の PhoK アルカリホスファターゼの機能解析． (2018)
  • Author(s): 高橋祥司・諸岡由佳・佐伯栞里・片沼拓士・阿部勝正・解良芳夫
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] Sphingobium sp. TCM1株における有機リン難燃剤分解代謝に関与するアルカリホスファターゼ遺伝子の同定 (2017)
  • Author(s): 高橋祥司・片沼拓士・阿部勝正・解良芳夫
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学（京都府、京都市）
  • Year and Date: 2017-03-19
• [Presentation] Enzymatic properties of PhoK alkaline phosphatase of Sphingobium sp. Strain TCM1. (2017)
  • Author(s): Yuka Morooka, Shouji Takahashi, Hiroshi Katanuma, Katsumasa Abe, Yoshio Kera
  • Organizer: The 6th international GIGAKU conference in Nagaoka (IGCN2017),
  • Int'l Joint Research
• [Presentation] Expression, affinity purification, and characterization of NH-tagged haloalkylphosphorus hydrolase. (2017)
  • Author(s): Narumi Tanaka，Katsue Miura，Katsumasa Abe，Shouji Takahashi，Yoshio Kera
  • Organizer: The 6th international GIGAKU conference in Nagaoka (IGCN2017),
  • Int'l Joint Research
• [Presentation] Functional analysis of a phosphodiesterase gene in the metabolism of an organophosphorus flame retardant in Sphingobium sp. strain TCM1. (2017)
  • Author(s): Masaya Morinaga, Shouji Takahashi, Katsumasa Abe, Yoshio Kera
  • Organizer: The 6th international GIGAKU conference in Nagaoka (IGCN2017),
  • Int'l Joint Research
• [Presentation] Regulation of phosphotriesterase gene expression in the organophosphorus flame retardant-degrading bacterium Sphingobium sp. strain TCM1. (2017)
  • Author(s): Takahito Kumakura, Shouji Takahashi, Maya Akimoto, Katsumasa Abe, Yoshio Kera
  • Organizer: The 6th international GIGAKU conference in Nagaoka (IGCN2017),
  • Int'l Joint Research
• [Presentation] 変異型ハロアルキル有機リン酸トリエステル加水分解酵素の諸特性解析． (2017)
  • Author(s): 田中成美・三浦克恵・西郡祐輝・阿部勝正・高橋祥司・解良芳夫
  • Organizer: 2017年度生命科学系学会合同年会（第90回日本生化学会）
• [Presentation] Sphingobium sp. TCM1株 PhoKアルカリホスファターゼの酵素学的諸特性の解析． (2017)
  • Author(s): 諸岡由佳・片沼拓士・阿部勝正・高橋祥司・解良芳夫
  • Organizer: 2017年度生命科学系学会合同年会（第90回日本生化学会）
• [Presentation] Study of the Locarization of Haloalkylphosphorus Hydrolase from Sphingobium sp. Strain TCM1. (2016)
  • Author(s): Toshihiro Imai, Takahiro Kabasawa, Katsumasa Abe, Shouji Takahashi and Yoshio Kera
  • Organizer: The 5th international GIGAKU conference in Nagaoka (IGCN2016)
  • Place of Presentation: 長岡技術科学大学（新潟県・長岡市）
  • Year and Date: 2016-10-07
  • Int'l Joint Research
• [Presentation] Study on the localization of Haloalkylphosphotriesterase from Sphingomonas sp. Strain TDK1. (2016)
  • Author(s): Risa Ibaraki, Toyokazu Kobayashi, Katsumasa Abe, Shouji Takahashi, Yoshio Kera
  • Organizer: The 5th international GIGAKU conference in Nagaoka (IGCN2016)
  • Place of Presentation: 長岡技術科学大学（新潟県・長岡市）
  • Year and Date: 2016-10-07
  • Int'l Joint Research
• [Presentation] Sphingobium sp. TCM1 株有機リン酸加水分解酵素中のシグナル様ペプチドの機能解析． (2016)
  • Author(s): 今井俊宏・李 誠駿・阿部勝正・高橋祥司・解良芳夫
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター・東北大学川内北キャンパス(宮城県・仙台市)
  • Year and Date: 2016-09-27
• [Presentation] Sphingomonads が有する有機リン酸加水分解酵素の局在解析． (2016)
  • Author(s): 茨木梨沙・櫻庭裕樹・阿部勝正・高橋祥司・解良芳夫
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター・東北大学川内北キャンパス(宮城県・仙台市)
  • Year and Date: 2016-09-27