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Development of novel nitrogen removal process from wastewater using denitrifying anaerobic methane oxidation reaction and investigation of microorganism involved in the reaction

Research Project

Project/Area Number 16H02975
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionNagaoka University of Technology

Principal Investigator

HATAMOTO Masashi  長岡技術科学大学, 産学融合トップランナー養成センター, 特任准教授 (20524185)

Co-Investigator(Kenkyū-buntansha) 山口 隆司  長岡技術科学大学, 工学研究科, 教授 (10280447)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywordsメタン / 亜酸化窒素 / 嫌気 / 脱窒反応 / メタン酸化 / 廃水処理 / 硝酸 / 亜硝酸 / DAMO / アーキア / 温室効果ガス / 排水・排ガス / 排水処理 / 嫌気的メタン酸化脱窒反応 / 廃棄物等発生抑制
Outline of Final Research Achievements

In this study, nitrogen removal process from wastewater using denitrifying anaerobic methane oxidation (DAMO) reaction was developed. The method has advantages of no organic carbon requirement, less sludge production and low-cost for construction and maintenance. However, their drawback is long time requirement for biomass growth. In order to overcome this drawback, this study used sponge carrier reactor named DHS and hallow fiber membrane reactor for its unique structure which facilitates the biofilm growth and the gas-liquid mass transfer. The results of this study showed that using DAMO combined with DHS showed lower N2O gas emission during the nitrogen removal compared to conventional nitrogen removal. Using hallow fiber membrane reactor, it showed faster reactor start up and we found different microbial groups were involved in nitrogen removal, which might be strongly influenced the inoculum source.

Academic Significance and Societal Importance of the Research Achievements

嫌気的メタン酸化脱窒反応では亜酸化窒素を経由しないため、従来の脱窒反応とは異なり、温室効果ガスである亜酸化窒素の大気放出が抑制できると考えられている。しかしながら、本研究では共存微生物からの亜酸化窒素放出が存在することを確認した。その一方で、従来法よりも亜酸化窒素放出量は少ないことも明らかにした。本成果は温室効果ガスの排出を抑制した新規な廃水からの窒素除去プロセスの開発に繋がる物である。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (7 results)

All 2019 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Effects of Copper and PQQ on the Denitrification Activities of Microorganisms Facilitating Nitrite- and Nitrate-Dependent DAMO Reaction2018

    • Author(s)
      Hatamoto Masashi、Nemoto Sho、Yamaguchi Takashi
    • Journal Title

      International Journal of Environmental Research

      Volume: 12 Issue: 5 Pages: 749-753

    • DOI

      10.1007/s41742-018-0118-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivation of denitrifying anaerobic methane-oxidizing microorganisms in a continuous-flow sponge bioreactor2017

    • Author(s)
      Masashi Hatamoto, Takafumi Sato, Sho Nemoto, Takashi Yamaguchi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 印刷中 Issue: 14 Pages: 5881-5888

    • DOI

      10.1007/s00253-017-8315-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Application of Hollow Fiber Membrane Reactor in Autotrophic Removal of Nitrogen2019

    • Author(s)
      Tran Phuong Thao, Hatamoto Masashi, Takahiro Watari, Takashi Yamaguchi
    • Organizer
      第53回 日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] DHSリアクターにおける嫌気的メタン酸化脱窒反応による亜酸化窒素発生量の評価2018

    • Author(s)
      津場大輔、吉田悠亮、根本笙、牧慎也、幡本将史、山口隆司
    • Organizer
      平成29年度土木学会全国大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 嫌気的メタン酸化脱窒反応による窒素除去と亜酸化窒素発生量の評価2017

    • Author(s)
      津場大輔、吉田悠亮、根本笙、牧慎也、幡本将史、山口隆司
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] DHSリアクターを用いた嫌気的メタン酸化脱窒反応による硝酸・亜硝酸の窒素除去2016

    • Author(s)
      津場大輔, 根本笙, 牧慎也, 幡本将史, 山口隆司
    • Organizer
      第34回土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      長岡
    • Year and Date
      2016-11-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] Application of denitrifying methane-oxidizing microorganisms for wastewater treatment using sponge bioreactor2016

    • Author(s)
      Masashi Hatamoto, Takafumi Sato, Shou Nemoto, Yusuke Yoshida, Takashi Yamaguchi
    • Organizer
      16th International Symposium on Microbial Ecology (ISME16)
    • Place of Presentation
      Montreal
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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