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Development of sludge property modification technology using PHA-accumulating bacteria for efficient anaerobic digestion

Research Project

Project/Area Number 16H03000
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Design and evaluation of sustainable and environmental conscious system
Research InstitutionOsaka University (2017-2018)
Kitasato University (2016)

Principal Investigator

INOUE Daisuke  大阪大学, 工学研究科, 准教授 (70448091)

Co-Investigator(Kenkyū-buntansha) 池 道彦  大阪大学, 工学研究科, 教授 (40222856)
黒田 真史  大阪大学, 工学研究科, 助教 (20511786)
清 和成  北里大学, 医療衛生学部, 教授 (80324177)
澤田 和子  北里大学, 医療衛生学部, 特別研究員 (00772485)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Keywordsバイオマス利活用 / 下水汚泥 / ポリヒドロキシアルカン酸 / 嫌気性消化 / 微生物解析
Outline of Final Research Achievements

This study clarified the distribution of Rhodocyclaceae, which would be a key player for increasing PHA contents, in activated sludge and their PHA accumulation ability. In particular, this study identified the carbon sources and the environmental conditions favorable for efficient PHA accumulation by Rhodocyclaceae members. This study also clarified that aerobic dynamic discharge process is a suitable method to enrich PHA-accumulating bacteria, especially Rhodocyclaceae, within a very short period. The findings obtained in this study will lead to the practical application of our proposed technology, which improves the energy recovery from waste activated sludge.

Academic Significance and Societal Importance of the Research Achievements

Rhodocyclaceae科細菌の下水汚泥中における分布やPHA蓄積能に関する知見はこれまでにほとんどなく、本研究で得たそれらの知見は学術的に新規性が高く、今後の関連研究を後押しする重要なものといえる。また、本研究で明らかにしたPHA蓄積菌の迅速集積法などは、下水汚泥からの資源・エネルギー回収に活用され得るポテンシャルを有していると考えられることから、本研究の成果は社会的にも大きな意義を有するものといえる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (16 results)

All 2019 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (14 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] Polyhydroxyalkanoate accumulation ability and associated microbial community in activated sludge-derived acetate-fed microbial cultures enriched under different temperature and pH conditions2018

    • Author(s)
      Daisuke Inoue, Yuta Suzuki, Kazuko Sawada, Kazunari Sei
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 125 Issue: 3 Pages: 339-345

    • DOI

      10.1016/j.jbiosc.2017.09.008

    • NAID

      40021507598

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polyhydroxybutyrate Accumulation Ability and Relevant Microbial Populations in Acetate-fed Mixed Microbial Cultures Enriched from Activated Sludge under Different Temperature Conditions2017

    • Author(s)
      Daisuke Inoue, Yuta Suzuki, Jota Morohoshi, Kazunari Sei
    • Journal Title

      Japanese Journal of Water Treatment Biology

      Volume: 53 Issue: 2 Pages: 57-67

    • DOI

      10.2521/jswtb.53.57

    • NAID

      130006471054

    • ISSN
      0910-6758, 1881-0438
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Zoogloeaceae科細菌のポリヒドロキシアルカン酸蓄積能に及ぼす環境因子の影響2019

    • Author(s)
      大津留知樹, 古川隼士, 清和成, 井上大介
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 余剰汚泥中のポリヒドロキシアルカン酸蓄積微生物の迅速集積に関する基礎的検討2019

    • Author(s)
      井上大介, 福山篤史, 池道彦
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Potential of PHA and glycogen accumulation from industrial wastewater in waste activated sludge2018

    • Author(s)
      Daisuke Inoue, Takaaki Narui, Yukihiro Okada, Kazunori Yokoe, Masashi Kuroda, Satoshi Soda, Michihiko Ike
    • Organizer
      Grand Renewable Energy 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Zoogloeaceae科細菌のポリヒドロキシアルカン酸(PHA)蓄積能に及ぼす炭素源の影響2018

    • Author(s)
      大津留知樹, 古川隼士, 井上大介, 清和成
    • Organizer
      第7回ファージ研究会・2018年日本細菌学会関東支部インターラボセミナー合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 二次処理方式に着目した下水処理場におけるメタン生成ポテンシャルの評価2018

    • Author(s)
      井上大介, 成井貴祥, 黒田真史, 惣田訓, 池道彦
    • Organizer
      第29回廃棄物資源循環学会研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rhodocyclaceae科細菌のポリヒドロキシアルカン酸(PHA)蓄積能の評価2018

    • Author(s)
      大津留知樹, 古川隼士, 井上大介, 清和成
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 下水二次処理方式が余剰汚泥のメタン回収ポテンシャルに及ぼす影響の評価2018

    • Author(s)
      成井貴祥, 井上大介, 黒田真史, 惣田訓, 池道彦
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 異なるpH・温度条件で活性汚泥から集積したPHA蓄積微生物群集の特徴づけ2017

    • Author(s)
      井上大介、鈴木悠太、澤田和子、清和成
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本大学黒髪キャンパス(熊本県熊本市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Polyhydroxyalkanoate accumulation abilities of Thauera and Zoogloea strains2017

    • Author(s)
      Daisuke Inoue, Tomoki Otsuru, Kazunari Sei
    • Organizer
      Water and Environment Technology Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Potential of waste activated sludge to accumulate intercellular polymeric substances from simple substrates and industrial wastewater2017

    • Author(s)
      Takaaki Narui, Yukihiro Okada, Kazunori Yokoe, Masashi Kuroda, Daisuke Inoue, Satoshi Soda, Michihiko Ike
    • Organizer
      10th Advanced Engineering Technology for Environment and Energy -Environment, Energy and Sustainable Development
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Polyhydroxyalkanoate accumulation abilities of Azoarcus, Thauera and Zoogloea strains2017

    • Author(s)
      Tomoki Otsuru, Daisuke Inoue, Kazunari Sei
    • Organizer
      10th Advanced Engineering Technology for Environment and Energy -Environment, Energy and Sustainable Development
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of length of feast period on PHB accumulation ability of activated sludge in acetate-fed SBR2017

    • Author(s)
      Daisuke Inoue, Kousuke Sakai, Sawa Miyake, Satoshi Soda, Michihiko Ike
    • Organizer
      the 2nd International Resource Recovery Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酢酸を基質として活性汚泥より集積した微生物群のPHB蓄積能に対するFeast期の影響2017

    • Author(s)
      井上大介, 酒井孝輔, 三宅佐和, 惣田訓, 池道彦
    • Organizer
      日本水処理生物学会第54回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Influence of temperature and pH during enrichment process on polyhydroxyalkanoate accumulation ability and associated microbial community of acetate-fed enrichment cultures derived from activated sludge2016

    • Author(s)
      Daisuke Inoue, Yuta Suzuki, Kazuko Sawada, Kazunari Sei
    • Organizer
      The 9th Annual Conference of the Challenges in Environmental Science and Engineering
    • Place of Presentation
      Grand Hi-Lai Hotel (Kaohsiung, Taiwan)
    • Year and Date
      2016-11-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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