| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
We investigated the circadian expression rhythm of a clock gene in cultured slices of the suprachiasmatic nuclei derived from gene-targeted mice exhibiting enhanced elongation in the period length of circadian locomotor rhythms under the constant lightness. In cultured slices derived from mice kept under the constant lightness, period lengths of clock gene expression rhythms became unstable compared with the light-dark cycle condition, but no significant difference was observed compared with control genotypes. Currently, we are investigating the difference in shortening the period length of circadian locomotor rhythms due to the rotating-wheel running.
Out of five enzymes of the urea cycle present in the liver, mice fed with the standard diet showed diurnal rhythms of mRNA levels only in two enzymes. On the other hand, with prolonged fasting, a high-carbohydrate (no-protein) diet, low-protein diets, and high-protein diets, diurnal rhythms were observed in most enzyme mRNA levels.
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