| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
|---|
| Outline of Final Research Achievements |
Methylated cytosine (5mC) is important for the regulation of transcription, and is involved in development, differentiation, carcinogenesis, etc. Furthermore, the importance of RNA methylation is also becoming clear. In this study, with the aim of developing a technique for DNA/RNA sequence-specific detection and control of the methylation status in living cells, I focused on the following points; (1) Development of DNA binding protein capable of selectively recognizing cytosine methylation in a specific genomic sequence, (2) detection of methylation in living cells using designed methylation discrimination protein, and (3) Detection and control of RNA methylation status.
|
