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Elucidation of the system to get success on land by using chemical weapons

Research Project

Project/Area Number 16H03283
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomolecular chemistry
Research InstitutionYamaguchi University

Principal Investigator

Matsui Kenji  山口大学, 大学院創成科学研究科, 教授 (90199729)

Co-Investigator(Kenkyū-buntansha) 肥塚 崇男  山口大学, 大学院創成科学研究科, 助教 (30565106)
河内 孝之  京都大学, 生命科学研究科, 教授 (40202056)
Research Collaborator Simamura Masaki  
Uefune Masayoshi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsゼニゴケ / テルペン合成酵素 / セスキテルペン / 油体細胞 / 陸上進化 / 防衛物質 / 二次代謝産物 / 集積機構 / 油体細胞分化 / 化学防衛 / 化学生態学 / 化学防衛物質 / 進化 / 生合成
Outline of Final Research Achievements

It is assumed that Marchantia polymorpha still keeps some of the original systems that were acquired during settling on land. Among those, harnessing chemical weapons, such as terpenoids, seem to be responsible for its success on lands. In this study, we aimed to figure out how M. polymorpha acquired the genes for chemical defense and how M. polymorpha improved the system to accumulate the chemicals in distinct cells. A deep data-mining with genome sequence of M. polymorpha yielded more than 30 genes for terpene synthase (TPS). Among them, those similar to fungal TPS were shown to be involved in sesquiterpene synthesis. It was assumed that the genes duplicated many times very recently. A promoter-assay with TdTomato showed that the fungal type TPSs specifically expressed in oil body cells. Accordingly, it was suggested that acquiring TPS from fungi and innovation of cell-factories specific to form and store terpenoids took an important role for settling on lands.

Academic Significance and Societal Importance of the Research Achievements

苔類は最初に陸上進出に成功し、他の植物系統が絶滅して行く中、陸域生態系を今日まで生き抜いてきた。その現生系統のゼニゴケは多様な化学防衛物質を構成的に持つことで高い防衛体制を常に有している。本研究ではそうした防衛体制が進化の過程でどのように獲得されてきたのかの一面を明らかにしたもので、その成果を活用することで新しい環境耐性強化植物の創成が期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (8 results)

All 2019 2018 2017 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] How Do Plants Emit and Take in Volatile Organic Chemicals?: Simple Diffusion Does not Illustrate the Mechanisms.2018

    • Author(s)
      松井健二、望月智史
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 56 Issue: 2 Pages: 95-103

    • DOI

      10.1271/kagakutoseibutsu.56.95

    • NAID

      130007563370

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2018-01-20
    • Related Report
      2017 Annual Research Report
  • [Journal Article] Identification of a Hexenal Reductase That Modulates the Composition of Green Leaf Volatiles2018

    • Author(s)
      Tanaka Toshiyuki、Ikeda Ayana、Shiojiri Kaori、Ozawa Rika、Shiki Kazumi、Nagai-Kunihiro Naoko、Fujita Kenya、Sugimoto Koichi、Yamato Katsuyuki T.、Dohra Hideo、Ohnishi Toshiyuki、Koeduka Takao、Matsui Kenji
    • Journal Title

      Plant Physiology

      Volume: 178 Issue: 2 Pages: 552-564

    • DOI

      10.1104/pp.18.00632

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 1-Octen-3-ol Is Formed from Its Glycoside during Processing of Soybean [Glycine max (L.) Merr.] Seeds2018

    • Author(s)
      Matsui Kenji、Takemoto Hiroyuki、Koeduka Takao、Ohnishi Toshiyuki
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 66 Issue: 28 Pages: 7409-7416

    • DOI

      10.1021/acs.jafc.8b01950

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Green leaf volatile-burst in Arabidopsis is governed by galactolipid oxygenation by a lipoxygenase that is under control of calcium ion2018

    • Author(s)
      Satoshi Mochizuki, Kenji Matsui
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 505 Pages: 939-944

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物の香り化合物による生存戦略2019

    • Author(s)
      松井健二
    • Organizer
      日本農薬学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 植物生態系を紡ぐ香り化合物の代謝を介した相互作用2019

    • Author(s)
      松井健二
    • Organizer
      理研シンポジウム「第19回分析・解析技術と化学の最先端」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Growth condition of Marchantia polymorpha affects number of oil body cells and amounts of secondary metabolites2017

    • Author(s)
      Kenji Matsui
    • Organizer
      The 65th NIBB Conference Marchantia Workshop 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 松井・肥塚研究室

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~matsui/

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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