Screening of amyloid aggregation inhibitors using quantum dot nanoprobe and elucidation of the inhibition mechanism

• Project/Area Number: 16H03288
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Muroran Institute of Technology
• Principal Investigator: Tokuraku Kiyotaka 室蘭工業大学, 大学院工学研究科, 准教授 (00332106)
• Co-Investigator(Kenkyū-buntansha): 中村 宗一郎 信州大学, 役員等, 理事 (00105305) ; 橋 友理香 山野美容芸術短期大学, その他部局等, 講師 (40756962) ; 上井 幸司 室蘭工業大学, 大学院工学研究科, 准教授 (80347905)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: アミロイド / 凝集阻害 / 量子ドット / ハイスループット / スクリーニング / Aβ / タウ / オリゴマー / アルツハイマー / シヌクレイン / 医薬品探索 / 認知症 / アルツハイマー病 / アミロイドβ / αシヌクレイン

Outline of Final Research Achievements

Amyloid disease such as Alzheimer's disease is triggered by aggregation of amyloid protein to develop it, and therefore the aggregation inhibitor is useful for prevention and treatment of disease. In this study, we developed an automated microliter-scale high-throughput screening system (MSHTS system) for amyloid β (Aβ) aggregation inhibitors applying real-time imaging method of Aβ aggregation using quantum dots. Furthermore, we screened for inhibitory substances using this automated system and analyzed the structure-activity relationship of active substances. In addition to expanding the MSHTS method to other amyloids (tau, α-synuclein, and amyloid A), we developed a new screening method for inhibitors of amyloid oligomer formation with high neurotoxicity.

Academic Significance and Societal Importance of the Research Achievements

認知症の過半数を占めるアルツハイマー病の発症には、アミロイドβやタウと呼ばれるタンパク質の脳内での凝集と蓄積が関与しているため、凝集を抑制する物質は病気の予防や治療に有用である。本研究では、不純物を含む天然物エキスのアミロイド凝集抑制活性であっても微量かつ効率的に評価できる自動分析システムの開発に成功し、本手法を用いて様々な凝集抑制物質を発見してきた。今後、本手法や新たに見つかった化合物を用いることで、認知症予防に関する研究が飛躍的に進展することが期待される。

Research Products

• [Journal Article] An automated microliter-scale high-throughput screening system (MSHTS) for real-time monitoring of protein aggregation using quantum-dot nanoprobes (2019)
  • Author(s): Sasaki Rina、Tainaka Reina、Ando Yuichi、Hashi Yurika、Deepak Hadya V.、Suga Yoshiko、Murai Yuta、Anetai Masaki、Monde Kenji、Ohta Kiminori、Ito Ikuko、Kikuchi Haruhisa、Oshima Yoshiteru、Endo Yasuyuki、Nakao Hitomi、Sakono Masafumi、Uwai Koji、Tokuraku Kiyotaka
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 2587-2587
  • DOI: 10.1038/s41598-019-38958-0
  • Peer Reviewed / Open Access
• [Journal Article] Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs (2018)
  • Author(s): Shigematsu Hideki、Imasaki Tsuyoshi、Doki Chihiro、Sumi Takuya、Aoki Mari、Uchikubo-Kamo Tomomi、Sakamoto Ayako、Tokuraku Kiyotaka、Shirouzu Mikako、Nitta Ryo
  • Journal Title: The Journal of Cell Biology Volume: 217 Issue: 12 Pages: 4155-4163
  • DOI: 10.1083/jcb.201711182
  • NAID: 120006599259
  • Peer Reviewed
• [Journal Article] Rosmarinic acid is a novel inhibitor for Hepatitis B virus replication targeting viral epsilon RNA-polymerase interaction (2018)
  • Author(s): Tsukamoto Yuta、Ikeda Sotaro、Uwai Koji、Taguchi Riho、Chayama Kazuaki、Sakaguchi Takemasa、Narita Ryo、Yao Wan-Ling、Takeuchi Fumihiko、Otakaki Yukie、Watashi Koichi、Wakita Takaji、Kato Hiroki、Fujita Takashi
  • Journal Title: PLOS ONE Volume: 13 Issue: 5 Pages: e0197664-e0197664
  • DOI: 10.1371/journal.pone.0197664
  • NAID: 120006649572
  • Peer Reviewed / Open Access
• [Journal Article] Emerging roles of bioactive peptides on brain health promotion (2018)
  • Author(s): Katayama Shigeru、Nakamura Soichiro
  • Journal Title: International Journal of Food Science & Technology Volume: 1 Issue: 6 Pages: 1-7
  • DOI: 10.1111/ijfs.14076
  • Peer Reviewed / Open Access
• [Journal Article] Biological and neuroprotective activity of Thai edible plant extracts (2018)
  • Author(s): Junsathian Pimmada、Yordtong Kanokwan、Corpuz Henry M.、Katayama Shigeru、Nakamura Soichiro、Rawdkuen Saroat
  • Journal Title: Industrial Crops and Products Volume: 124 Pages: 548-554
  • DOI: 10.1016/j.indcrop.2018.08.008
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Soybean-Derived Glycine-Arginine Dipeptide Administration Promotes Neurotrophic Factor Expression in the Mouse Brain (2018)
  • Author(s): Shimizu Ayano、Mitani Takakazu、Tanaka Sachi、Fujii Hiroshi、Maebuchi Motohiro、Amiya Yusuke、Tanaka Mitsuru、Matsui Toshiro、Nakamura Soichiro、Katayama Shigeru
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 66 Issue: 30 Pages: 7935-7941
  • DOI: 10.1021/acs.jafc.8b01581
  • Peer Reviewed
• [Journal Article] Long-Term Diet Supplementation with Lactobacillus paracasei K71 Prevents Age-Related Cognitive Decline in Senescence-Accelerated Mouse Prone 8 (2018)
  • Author(s): Corpuz Henry、Ichikawa Saki、Arimura Misa、Mihara Toshihiro、Kumagai Takehisa、Mitani Takakazu、Nakamura Soichiro、Katayama Shigeru
  • Journal Title: Nutrients Volume: 10 Issue: 6 Pages: 762-762
  • DOI: 10.3390/nu10060762
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Theophylline suppresses interleukin-6 expression by inhibiting glucocorticoid receptor signaling in pre-adipocytes. (2018)
  • Author(s): Mitani T, Takaya T, Harada N, Katayama S, Yamaji R, Nakamura S, Ashida H.
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 646 Pages: 98-106
  • DOI: 10.1016/j.abb.2018.04.001
  • NAID: 120007099784
  • Peer Reviewed
• [Journal Article] Kale supplementation up-regulates HSP70 and suppresses cognitive decline in a mouse model of accelerated senescence (2018)
  • Author(s): Kushimoto Shoko、Uchibori Yuki、Yanai Shuichi、Makabe Hidefumi、Nakamura Soichiro、Katayama Shigeru
  • Journal Title: Journal of Functional Foods Volume: 44 Pages: 292-298
  • DOI: 10.1016/j.jff.2018.03.011
  • Peer Reviewed
• [Journal Article] Cyperenoic acid suppresses osteoclast differentiation and delays bone loss in a senile osteoporosis mouse model by inhibiting non-canonical NF-κB pathway (2018)
  • Author(s): Chawalitpong Supatta、Chokchaisiri Ratchanaporn、Suksamrarn Apichart、Katayama Shigeru、Mitani Takakazu、Nakamura Soichiro、Athamneh Ahmad AI、Ritprajak Patcharee、Leelahavanichkul Asada、Aeimlapa Ratchaneevan、Charoenphandhu Narattaphol、Palaga Tanapat
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 5625-5625
  • DOI: 10.1038/s41598-018-23912-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure-activity relations of rosmarinic acid derivatives for the amyloid beta aggregation inhibition and antioxidant properties (2017)
  • Author(s): Taguchi Riho, Hatayama Koki, Takahashi Tomohito, Hayashi Takafumi, Sato Yuki, Sato Daisuke, Ohta Kiminori, Nakano Hiroto, Seki Chigusa, Endo Yasuyuki, Tokuraku Kiyotaka, Uwai Koji
  • Journal Title: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY Volume: 138 Pages: 1066-1075
  • DOI: 10.1016/j.ejmech.2017.07.026
  • Peer Reviewed
• [Journal Article] Unidirectional growth of heavy meromyosin (HMM) clusters along actin filaments revealed by real-time fluorescence microscopy (2017)
  • Author(s): Rika Hirakawa, Yusuke Nishikawa, Taro Q.P. Uyeda, Kiyotaka Tokuraku
  • Journal Title: Cytoskeleton Volume: 74 Issue: 12 Pages: 482-489
  • DOI: 10.1002/cm.21408
  • Peer Reviewed
• [Journal Article] Solventless extraction methods for immature fruits: Evaluation of their antioxidant and cytoprotective activities (2017)
  • Author(s): Heng, M.Y., Katayama, S., Mitani, T., Ong, E.S., Nakamura, S.
  • Journal Title: Food Chem. Volume: 221 Pages: 1388-1393
  • DOI: 10.1016/j.foodchem.2016.11.015
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 量子ドットナノプローブを用いたアミロイド凝集過程のリアルタイムイメージング技術とその活用 (2016)
  • Author(s): 德樂清孝、上井幸司
  • Journal Title: 第54回粉体に関する討論会講演論文集 Volume: 1 Pages: 136-140
  • NAID: 120006671919
  • Acknowledgement Compliant
• [Journal Article] 量子ドットを用いたAβ凝集阻害物質の微量ハイスループットスクリーニング法 (2016)
  • Author(s): 德樂清孝、上井幸司
  • Journal Title: BIO Clinica 31 Volume: 31 Pages: 76-81
• [Journal Article] Mutually exclusive cooperative binding of myosin and cofilin to actin filaments involves cooperative conformational changes of actin. (2016)
  • Author(s): Ngo KX, Umeki N, Kijima ST, Kodera N, Ueno H, Furutani-Umezu N, Nakajima J, Noguchi TQP, Nagasaki A, Tokuraku K, Uyeda TQP
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 35449-35449
  • DOI: 10.1038/srep35449
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Simultaneous analysis of major ingredients of Gardenia fruit by HPLC-MS/TQMS method (2016)
  • Author(s): Galaqin, M., Uwai, K., Yuguchi, M., Iwasa, T.
  • Journal Title: Mongolian J. Chem. Volume: 17 Pages: 34-37
  • NAID: 120006529878
  • Peer Reviewed
• [Journal Article] Simple primary β-amino alcohol catalyzed enantioselective Diels-Alder reaction of 3-hydroxy-2-pyridones (2016)
  • Author(s): Takahashi, T., Reddy, U.V.S., Kohari, Y., Seki, C., Furuyama, T., Kobayashi, N., Okuyama, Y., Kwon, E., Uwai, U., Tokiwa, M., Takeshita, M., Nakano, H.
  • Journal Title: Tetrahedron Letters Volume: 57 Issue: 51 Pages: 5771-5776
  • DOI: 10.1016/j.tetlet.2016.11.030
  • Peer Reviewed
• [Journal Article] 2-Azanorbornane-based amine organocatalyst for enantioselective aldol reaction of isatins with ketones (2016)
  • Author(s): Ogasawara, A., Reddy, U.V.S., Seki, C., Okuyama, Y., Uwai, K., Tokiwa, M., Takeshita, M., Nakano, H.
  • Journal Title: Tetrahedron: Asymmetry Volume: 27 Issue: 20-21 Pages: 1062-1068
  • DOI: 10.1016/j.tetasy.2016.08.013
  • Peer Reviewed
• [Journal Article] Simple Primary Amino Amide Organocatalyst for Enantioselective Aldol Reactions of Isatins with Ketones (2016)
  • Author(s): Kimura, J., Reddy, V.S., Kohari, Y., Seki, C., Mawatari, Y., Uwai, K., Okuyama, Y., Kwon, E., Tokiwa, M., Takeshita, M., Iwasa, T., Nakano, H.
  • Journal Title: European Journal of Organic Chemistry Volume: 2016 Pages: 3748-3756
  • Peer Reviewed
• [Journal Article] Effects of Sesaminol Feeding on Brain Aβ Accumulation in a Senescence-Accelerated Mouse-Prone 8 (2016)
  • Author(s): Katayama, S., Sugiyama, H., Kushimoto, S., Uchiyama, Y., Hirano, M., Nakamura, S
  • Journal Title: J. Agric. Food Chem. Volume: 64 Issue: 24 Pages: 4008-4013
  • DOI: 10.1021/acs.jafc.6b01237
  • Peer Reviewed
• [Presentation] 北海道の天然資源で認知症を予防する アミロイド凝集阻害物質の微量探索システムの開発と北海道天然資源からの有用物質の探索 (2019)
  • Author(s): 徳樂清孝
  • Organizer: 日本鉄道電気技術協会北海道支部講演会
  • Invited
• [Presentation] Elucidation of the aggregation of serum amyloid A protein and screening of the inhibitors using a microliter-scale highthroughput screening system (2019)
  • Author(s): Xuguang Lin, Masahiro Kuragano, Kenichi Watanabe, Kiyotaka Tokuraku
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2019
  • Int'l Joint Research
• [Presentation] Imaging of alpha-synuclein aggregates using quantum-dot nanoprobes (2019)
  • Author(s): Nuomin, Masahiro Kuragano, Taro Q.P. Noguchi, Kiyotaka Tokuraku
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2019
  • Int'l Joint Research
• [Presentation] Development of an automated microliter-scale highthroughput screening system with quantum-dot nanoprobes for amyloid aggregation inhibitors (2019)
  • Author(s): Rina Sasaki, Kenji Monde, Koji Uwai, Kiyotaka Tokuraku
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2019
  • Int'l Joint Research
• [Presentation] Synergistic effects of inhibition of amyloid β aggregation and induction of brain-derived neurotrophic factor (BDNF) activity on mitigation of neurocytotoxicity by amyloid β (2019)
  • Author(s): Masahiro Kuragano, Mamoru Fukuchi, Koji Uwai, Kiyotaka Tokuraku
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2019
  • Int'l Joint Research
• [Presentation] ゲンノショウコ (Geranium thunbergii) のアミロイトβ凝集阻害物質 (2019)
  • Author(s): 上井幸司，久保研二，渡邉輝，関千草，中野博人，徳樂清孝
  • Organizer: 日本薬学会第139年会
• [Presentation] アミロイド凝集阻害物質の自動スクリーニングシステムの開発と天然物および化合物ライブラリーの評価 (2019)
  • Author(s): 佐々木里奈、田井中玲奈、安藤雄一、太田公規、門出健次、上井幸司、徳樂清孝
  • Organizer: 日本生物物理学会北海道支部会
• [Presentation] カフェ酸誘導体によるアミロイドβ可溶性オリゴマー形成阻害の分光学的解析 (2019)
  • Author(s): 上井幸司，久米田博之，坂本和，関千草，中野博人，馬渡康輝，徳樂清孝
  • Organizer: 化学系学協会北海道支部2019年冬季研究発表会
• [Presentation] バイオナノイメージング法を応用した抗認知症物質の微量探索システム開発 (2018)
  • Author(s): 徳樂清孝
  • Organizer: 第30回フロンティア技術討論会
  • Invited
• [Presentation] Neuromodulatory effects of soybean-derived peptides on BDNF expression and neurogenesis (2018)
  • Author(s): Shigeru Katayama, Takakazu Mitani, Soichiro Nakamura
  • Organizer: ISNFF2018
  • Int'l Joint Research / Invited
• [Presentation] Functional properties of novel ferulicacis-rutinoside enzymatically synthesized using rutinose (2018)
  • Author(s): Kanako Matsumoto, Takakazu Mitani, HyunYoung Park, Dhiraj A. Vattem, Soichiro Nakamura, Shigeru Katayama
  • Organizer: ISNFF2018
  • Int'l Joint Research / Invited
• [Presentation] Bioactive peptides for brain health and its mechanistic exploration (2018)
  • Author(s): Shigeru Katayama, Takakazu Mitani, Soichiro Nakamura
  • Organizer: AOCS Annual Meeting & Expo 2018
  • Int'l Joint Research
• [Presentation] A microliter-scale high-throughput screening system of amyloid-β aggregation inhibitors (2018)
  • Author(s): Kiyotaka Tokuraku, Koji Uwai
  • Organizer: The 26th Annual Meeting of International Congress on Nutrition and Integrative Medicine (ICNIM 2018)
  • Int'l Joint Research
• [Presentation] アミロイドβ凝集阻害活性スクリーニングと活性物質の探索 (2018)
  • Author(s): 久保研二，渡邉輝，関千草，中野博人，徳樂清孝，上井幸司
  • Organizer: 第36回メディシナルケミストリーシンポジウム
• [Presentation] 量子ドットナノプローブを用いたアミロイド凝集阻害物質の自動化スクリーニングシステムの開発 (2018)
  • Author(s): 佐々木里奈、安藤雄一、上井幸司、徳楽清孝
  • Organizer: 第91回日本生化学会大会
• [Presentation] 量子ドットナノプローブを用いたtau凝集過程のリアルタイムイメージングと凝集阻害物質の活性評価 (2018)
  • Author(s): 田井中玲奈、橋友理香、中尾仁美、迫野昌文、徳楽清孝
  • Organizer: 第91回日本生化学会大会
• [Presentation] 量子ドットナノプローブを用いたα-シヌクレイン凝集体のイメージング (2018)
  • Author(s): NUOMIN、野口太郎、徳楽清孝
  • Organizer: 第91回日本生化学会大会
• [Presentation] 量子ドットを用いたTauリピートペプチド凝集阻害物質探索システムの開発と改良 (2018)
  • Author(s): 橋友理香、田井中玲奈、上井幸司、徳楽清孝
  • Organizer: 第91回日本生化学会大会
• [Presentation] アミロイドβ の凝集はアクチンに富む細胞辺縁部で凝集が促進される (2018)
  • Author(s): 近井優作、山下涼太、倉賀野正弘、高橋正行、徳楽清孝
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] 北海道白糠町産植物からのAβ凝集阻害物質の探索と機能性評価 (2018)
  • Author(s): 渡邉輝、久保研二、杉村康司、大場慶司、上井幸司、徳樂清孝
  • Organizer: 第70回日本生物工学会大会
• [Presentation] アミロイドβ―カフェ酸誘導体複合体の分光学的解析 (2018)
  • Author(s): 坂本和，久米田博之，杉浦亜美，馬渡康輝，田口莉帆，関千草，中野博人，徳楽清孝，上井幸司
  • Organizer: 日本化学会北海道支部2018年夏季研究発表会
• [Presentation] 醤油に含まれるアミロイドβ凝集阻害物質の探索 (2018)
  • Author(s): 吉成航、上井幸司、徳楽清孝
  • Organizer: 日本食品化学学会第24回総会・学術大会
• [Presentation] 北海道産ゲンノショウコ(Geranium thunbegii)からのアミロイド β 凝集阻害物質の探索 (2018)
  • Author(s): 久保研二、渡邉輝、関千草、中野博人、徳樂清孝、上井幸司
  • Organizer: 日本薬学会第138年会
• [Presentation] アミロイドβとカフェ酸エステル誘導体型凝集阻害剤の核磁気共鳴法による相互作用解析 (2018)
  • Author(s): 浦亜美、馬渡康輝、田口莉帆、関千草、中野博人、徳樂清孝、上井幸司
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] ジベンジリデンアセトン類のフェノール性水酸基がAβ凝集阻害活性に及す影響 (2017)
  • Author(s): 畑山晃輝、田口莉帆、安藤雄一、関千草、中野博人、徳樂清孝、上井幸司
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 宮城県、仙台国際センター
  • Year and Date: 2017-03-24
• [Presentation] アミロイドβ凝集阻害活性と総ポリフェノール含有量に基づく北海道白糠町産植物からの活性物質の探索 (2017)
  • Author(s): 久保研二、渡邉輝、杉村康司、関千草、中野博人、菱田敦之、徳樂清孝、上井幸司
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 宮城県、仙台国際センター
  • Year and Date: 2017-03-24
• [Presentation] Screening of amyloid-β aggregation inhibitors from aromatic low molecular compounds by a microliter-scale high-throughput screening system with quantam-dot nanoprobes (2017)
  • Author(s): uichi Ando, Kiminori Ota, Ikuko Ito, Haruhisa Kikuchi, Yoshiteru Oshima, Yasuyuki Endo, Koji Uwai, Kiyotaka Tokuraku
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Molecular design of novel phenylpropanoid-derived anti-amyloidal compounds using amyloidogenic peptide-linked quantum dot probe (2017)
  • Author(s): Soichiro Nakamura, Masaki Nakabayashi, Noriko Tanji, Takakazu Mitani, Kiyotaka Tokuraku, Shigeru Katayama
  • Organizer: The 10th International Conference and Exhibition on Nutraceuticals & Functional Foods (ISNFF2017)
  • Int'l Joint Research
• [Presentation] Exploring the active substances based on total polyphenol content and amyloid-β aggregation inhibitory activity of traditional beneficial plants in Hokkaido, Japan (2017)
  • Author(s): Koji Uwai, Kenji Kubo, Hikaru Watanabe, Kiyotaka Tokuraku
  • Organizer: The 8th Forum on Studies of Environmental and Public Health Issues in the Asian mega-Cities (EPAM 2017)
  • Int'l Joint Research
• [Presentation] 量子ドットナノプローブを用いたTauリピートペプチドによるアミロイド線維形成過程の経時的観察 (2017)
  • Author(s): 橋友理香、田井中玲奈、上井幸司、徳楽清孝
  • Organizer: 第90回日本生化学会大会
• [Presentation] アミロイドβ（Aβ）凝集体の形態に与えるAβ凝集阻害物質の効果の3D観察 (2017)
  • Author(s): 楊卓朗、板田鉄平、大柄俊貴、上井幸司、徳楽清孝
  • Organizer: 第69回日本生物工学会大会
• [Presentation] 北海道白糠町産植物からのアミロイドβ凝集阻害物質の網羅的探索 (2017)
  • Author(s): 渡邉輝、久保研二、杉村康司、徳楽清孝、上井幸司
  • Organizer: 第69回日本生物工学会大会
• [Presentation] 量子ドットナノプローブを用いたタウ凝集過程の可視化と凝集阻害物質の活性評価 (2017)
  • Author(s): 田井中玲奈、松井一史、橋友理香、上井幸司、徳楽清孝
  • Organizer: 日本食品化学学会第23回総会・学術大会
• [Presentation] Aβ凝集阻害物質の微量ハイスループットスクリーニング法を用いた市販ドレッシングの活性評価 (2017)
  • Author(s): 吉成 航、上井幸司、徳楽清孝
  • Organizer: 日本食品化学学会第23回総会・学術大会
• [Presentation] Discovery of lead compounds targeting Amyloid-β aggregation from aromatic low-molecular compounds (2017)
  • Author(s): Ando, Y., Ota, K., Ito, I., Kikuchi, H., Oshima, Y., Endo, Y., Uwai, K., Tokuraku, K.
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2017
  • Place of Presentation: 北海道、室蘭工業大学
  • Int'l Joint Research
• [Presentation] Suppression of Amyloid β-induced cytotoxicity by Plant extracts and Polyphenols with inhibitory activity for Amyloid β aggregation (2017)
  • Author(s): Yamashita, R., Itada, T., Nakagawa, H., Uwai, K., Tokuraku, K.
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2017
  • Place of Presentation: 北海道、室蘭工業大学
  • Int'l Joint Research
• [Presentation] Function of Gloiopeltis furcata as Aggregation Inhibitor for Aβ, evaluated by a microliter-scale high-throughput screening system (2017)
  • Author(s): Yo, T., Itada, T., Takahashi, T., Ogara, T., Uwai, K., Tokuraku, K.
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2017
  • Place of Presentation: 北海道、室蘭工業大学
  • Int'l Joint Research
• [Presentation] Structure-activity Relationship of Amyloid β Aggregation Inhibitors: Dibenzylideneacetone Derivatives (2017)
  • Author(s): Hatayama, K., Taguchi, R., Ando, Y., Seki, C., Nakano, H., Tokuraku, K., Uwai, K.
  • Organizer: Joint Seminar on Environmental Science and Disaster Mitigation Research 2017
  • Place of Presentation: 北海道、室蘭工業大学
  • Int'l Joint Research
• [Presentation] メカニズムに着目したカフェイン酸誘導体のアミロイドβ凝集阻害活性評価 (2016)
  • Author(s): 田口莉帆、高橋倫人、畑山晃輝、太田公規、橋友理香、関千草、中野博人、遠藤泰之、徳樂清孝、上井幸司
  • Organizer: 第34回メディシナルケミストリーシンポジウム
  • Place of Presentation: 茨城県、つくば国際会議場
  • Year and Date: 2016-11-30
• [Presentation] Presumption of amyloid-β aggregation mechanism based on the structure-activity relationships for aggregation inhibition and docking studies (2016)
  • Author(s): TAGUCHI, R., TAKAHASHI, T., KOSAKA, S., TOKURAKU, K., UWAI, K.
  • Organizer: The 44th symposium on structure-activity relationship and the 32st assembly for pesticide design research
  • Place of Presentation: 京都府、京都大学医学部
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] ショウジョウバエ株化神経細胞BG2-c6を用いたアミロイドβ神経細胞毒性評価 (2016)
  • Author(s): 山下涼太、板田鉄平、中川裕之、上井幸司、徳楽清孝
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山県、富山国際会議場
  • Year and Date: 2016-09-28
• [Presentation] Imaging of amyloid aggregation by quantum dots and its application for screening of aggregation inhibitors (2016)
  • Author(s): 徳楽清孝
  • Organizer: 第67回コロイドおよび界面化学討論会
  • Place of Presentation: 北海道、北海道教育大学旭川校
  • Year and Date: 2016-09-22
  • Int'l Joint Research
• [Presentation] 量子ドットナノプローブを用いたアミロイド凝集過程のリアルタイムイメージング技術とその活用 (2016)
  • Author(s): 徳樂清孝、上井幸司
  • Organizer: 第54回粉体に関する討論会
  • Place of Presentation: 北海道、登別グランドホテル
  • Year and Date: 2016-09-12
• [Presentation] Effects of seaweeds extracts for amyloid β aggregation (2016)
  • Author(s): Takahashi, T., Yo, T., Ogara, T., Yasui, H., Uwai, K., Tokuraku, K.
  • Organizer: The 7th Forum on Studies of Environmental and Public Health Issues in the Asian mega-Cities
  • Place of Presentation: 北海道、室蘭工業大学
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Effect of the number and position of phenolic hydroxyl groups on the Aβ aggregation inhibition of polyphenols (2016)
  • Author(s): Hatayama, K., Taguchi, R., Takahashi, T., Seki, C., Nakano, H., Tokuraku, K., Uwai, K.
  • Organizer: The 7th Forum on Studies of Environmental and Public Health Issues in the Asian mega-Cities
  • Place of Presentation: 北海道、室蘭工業大学
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Inhibitory mode of rosmarinic acid derivatives against amyloid - beta aggregation: their ThT and MSHTS analyses (2016)
  • Author(s): Taguchi, R., Takahashi, T., Hatayama, K., Hashi, Y., Seki, C., Nakano, H., Tokuraku, K., Uwai, K.
  • Organizer: The 7th Forum on Studies of Environmental and Public Health Issues in the Asian mega-Cities
  • Place of Presentation: 北海道、室蘭工業大学
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] 微量ハイスループットスクリーニング法を用いたフクロフノリ（Gloiopeltis furcata）熱水抽出物のAβ凝集阻害機構の解明 (2016)
  • Author(s): 楊卓朗、板田鉄平、高橋倫人、大柄俊貴、上井幸司、徳楽清孝
  • Organizer: 日本食品化学学会第22回総会・学術大会
  • Place of Presentation: 高知県、高知市文化プラザかるぽーと
  • Year and Date: 2016-06-02
• [Presentation] 多様なアミロイド形成のモニターを可能にする新規量子ドットプローブの創製 (2016)
  • Author(s): 中林昌基、片山茂、三谷塁一、徳楽清孝、橋友理香、中村宗一郎
  • Organizer: 日本農芸化学会中部支部、第177回例会
  • Place of Presentation: 愛知県、名古屋大学農学部
• [Remarks] 徳樂研究室Webサイト
  • URL: http://www3.muroran-it.ac.jp/tokuraku/tokuraku/Top_Page.html
• [Patent(Industrial Property Rights)] アミロイド形成を評価する方法、装置及びプログラム (2018)
  • Inventor(s): 徳楽清孝、上井幸司
  • Industrial Property Rights Holder: 徳楽清孝、上井幸司
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018