| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
I previously reported that a post-translational modification of a protein is closely related to degradation of intracellular bacteria. Here, I hypothesized that this modification could work as a degradation-inducing tag also for non-bacterial targets. Various guanine derivatives were synthesized and When we introduced it into the protein and examined its effect on the protein lifespan, we found that, as expected We have discovered a derivative that shortens the protein lifetime. The mechanism of action was analyzed using various mutant cell lines related to autophagy. In addition, the generalizability of the effect was verified by increasing the number of examples of proteins subjected to degradation.
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