Sptiotemporal dynamics of brain structure and connection in brain injury and plasticity

• Project/Area Number: 16H03300
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic / Social brain science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hayashi Takuya 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (50372115)
• Co-Investigator(Kenkyū-buntansha): 山本 竜也 つくば国際大学, 医療保健学部, 助教 (60724812) ; 合瀬 恭幸 国立研究開発法人理化学研究所, 生命機能科学研究センター, 専門技術員 (70519404) ; 伊佐 正 京都大学, 医学研究科, 教授 (20212805)
• Research Collaborator: Noriyuki Higo
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: 可塑性 / 拡散MRI / 連絡性 / 神経突起 / 神経連絡性 / 拡散強調MRI画像 / 安静時機能的MRI画像 / 神経トレーサー / MRI / 拡散強調 / 安静時脳活動 / 脳コネクトーム / 拡散強調画像 / 機能回復 / コネクトーム / 拡散トラクトグラフィー / 脳損傷 / ネットワーク / 安静時MRI

Outline of Final Research Achievements

This research investigated the dynamics of brain function and structure by non-invasive imaging techniques such as MRI in order to answer the question of “the mechanism of brain damage and plasticity” which is involved after dysfunction of a specific system or part of the brain like Parkinson’s disease and stroke. We found changes over time or differences of brain organization with the control group in primate animal models, and visualized changes in intra-brain connectivity at the macroscopic level. During this process, we also developed high-resolution, sensitive, and reproducible technologies for MRI data acquisitions and preprocessing. At the end of the experiment, neurites at the micro level were also visualized by light microscopy to infer the neuronal plastic changes. From these multi-level approach, it was revealed that changes in functional and structural connections occur dynamically in a large scale of micro to macroscopic space throughout the brain.

Academic Significance and Societal Importance of the Research Achievements

本成果は、これまでに十分解明されていない霊長類脳の可塑性機構についてマクロスケールからミクロスケールにわたり全脳検索に挑戦した結果得られたものであり、従来考えられた以上に独特かつ複雑なパターンで可塑性が生じていることが明らかになった。非侵襲画像技術の高精度技術の開発も進んだことで、本技術を近い将来に臨床現場へ拡張することも可能であり脳疾患の病態把握や診断に寄与することが期待される。また本成果はリハビリなどの神経可塑性を促進する治療メカニズムの理解や新しい治療法の開発につながると考えらえる。今後はさらに画像技術を高精度化し可塑性機構のうちの各連絡網の因果的役割を明らかにしていくことが期待される。

Research Products

• [Int'l Joint Research] セントルイス・ワシントン大学(米国)
• [Int'l Joint Research] オックスフォード大学(英国)
• [Int'l Joint Research] ロンドン大学(英国)
• [Int'l Joint Research] ワシントン大学セントルイス(米国)
• [Journal Article] Does the Diffusion Tensor Model Predict the Neurite Distribution of Cerebral Cortical Gray Matter? ? Cortical DTI-NODDI (2018)
  • Author(s): Hikaru Fukutomi, Matthew F. Glasser, Katsutoshi Murata, Thai Akasaka, Koji Fujimoto, Takayuki Yamamoto, Joonas A. Autio, Tomohisa Okada, Kaori Togashi, Hui Zhang, David C. Van Essen, Takuya Hayashi
  • Journal Title: biorxiv Volume: -
  • DOI: 10.1101/441659
  • Open Access / Int'l Joint Research
• [Journal Article] Neurite imaging reveals microstructural variations in human cerebral cortical gray matter (2018)
  • Author(s): Fukutomi, H., Glasser, M.F., Zhang, H., Autio, J.A., Coalson, T.S., Okada, T., Togashi, K., Van Essen, D.C., and Hayashi, T.
  • Journal Title: Neuroimage Volume: in press Pages: 488-499
  • DOI: 10.1016/j.neuroimage.2018.02.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human iPS cell-derived dopaminergic neurons function in a primate Parkinson’s disease model (2017)
  • Author(s): Kikuchi Tetsuhiro、Morizane Asuka、Doi Daisuke、Magotani Hiroaki、Onoe Hirotaka、Hayashi Takuya、Mizuma Hiroshi、Takara Sayuki、Takahashi Ryosuke、Inoue Haruhisa、Morita Satoshi、Yamamoto Michio、Okita Keisuke、Nakagawa Masato、Parmar Malin、Takahashi Jun
  • Journal Title: Nature Volume: 548 Issue: 7669 Pages: 592-596
  • DOI: 10.1038/nature23664
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MHC matching improves engraftment of iPSC-derived neurons in non-human primates. (2017)
  • Author(s): Morizane A, Kikuchi T, Hayashi T, Mizuma H, Takara S, Doi H, Mawatari A, Glasser MF, Shiina T, Ishigaki H, Itoh Y, Okita K, Yamasaki E, Doi D, Onoe H, Ogasawara K, Yamanaka S, Takahashi J.
  • Journal Title: Nat Commun Volume: 8 Issue: 1 Pages: 385-385
  • DOI: 10.1038/s41467-017-00926-5
  • NAID: 120006365783
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional, and Structural Imaging of Major Depressive Disorder (2016)
  • Author(s): Zhang Kai, Zhu Yunqi, Zhu Yuankai, Wu Shuang, Liu Hao, Zhang Wei, Xu Caiyun, Zhang Hong, Hayashi Takuya, Tian Mei
  • Journal Title: Neuroscience bulletin Volume: 32(3) Pages: 273-85
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ヒト脳の解明のための霊長類脳コネクトーム研究 (2019)
  • Author(s): Hayashi Takuya
  • Organizer: 第21回日本ヒト脳機能マッピング学会
  • Invited
• [Presentation] Towards the Macaque Connectome: 24-Channel 3T Multi-Array Coil, MR Sequences, and HCP-style Preprocessing (2018)
  • Author(s): Autio Joonas, Ose Takayuki, Ohno Masahiro, Kawabata Yoshihiko, Urushibata Yuta , Nishigori Kantaro, Yamaguchi Masataka, Hori Yuki, Yoshida Atsushi, Murata Katsutoshi, Glasser Matthew F, Hayashi Takuya.
  • Organizer: ISMRM2018
  • Int'l Joint Research
• [Presentation] Resting state functional corticostriatal connectivity in Parkinsonian monkeys (2018)
  • Author(s): Autio Joonas, Ose Takayuki, Nishigori Kantaro, Tanki Nobuyoshi, Takahashi Jun, Glasser Matthew F, Hayashi Takuya
  • Organizer: ISMRM2018
  • Int'l Joint Research
• [Presentation] Development of a 24-Channnel 3T Multi-Array Coil for functional MRI in awake monkeys (2018)
  • Author(s): Yoshida Atsushi, Hori Yuki, Nishigori Kantaro, Ohno Masahiro, Kawabata Yoshihiko, Murata Katsutoshi, Urushibata Yuta , Yamaguchi Masataka, Autio Joonas, Glasser Matthew F, Hayashi Takuya
  • Organizer: ISMRM2018
  • Int'l Joint Research
• [Presentation] Translating the Human Connectome Project to Marmoset Imaging (2018)
  • Author(s): Hori Yuki, Autio Joonas, Ohno Masahiro, Kawabata Yoshihiko, Urushibata Yuta , Murata Katsutoshi, Yamaguchi Masataka, Kawasaki Akihiro, Takeda Chiho, Yokoyama Chihiro, Glasser Matthew F, Hayashi Takuya
  • Organizer: ISMRM2018
  • Int'l Joint Research
• [Presentation] Resting State Functional CorticoStriatal Connectivity in Parkinsonian Monkeys (2018)
  • Author(s): Autio Joonas, Nishigori Kantaro, Tanki Nobuyoshi, Yamaguchi Masataka, Takahashi Jun, Glasser Matthew F, Hayashi Takuya
  • Organizer: 第41回日本神経科学学会
• [Presentation] Resting state functional corticostriatal connectivity in Parkinsonian monkeys (2018)
  • Author(s): Autio Joonas, Ose Takayuki, Nishigori Kantaro, Tanki Nobuyoshi, Takahashi Jun, Glasser Matthew F, Hayashi Takuya
  • Organizer: 第2回ヒト脳イメージング研究会
• [Presentation] Development of a 24-Channel Multi-Array Coil for functional MRI at 3T in awake macaques (2018)
  • Author(s): Yoshida Atsushi, Hori Yuki, Nishigori Kantaro, Ohno Masahiro, Kawabata Yoshihiko, Urushibata Yuta , Murata Katsutoshi, Yamaguchi Masataka, Autio Joonas, Glasser Matthew F, Hayashi Takuya
  • Organizer: 第2回ヒト脳イメージング研究会
• [Presentation] Dopaminergic modulation of functional connectome in anesthetized macaque monkeys (2018)
  • Author(s): Nishigori Kantaro, Ose Takayuki, Ohno Masahiro, Yamaguchi Masataka, Autio Joonas, Yoshida Atsushi, Negishi Toru, NAKAZAWA Shunsuke, urushino Naoko, Ichihara Junji, Hayashi Takuya
  • Organizer: 第2回ヒト脳イメージング研究会
• [Presentation] Translating Human Connectome Project to Marmoset Imaging (2018)
  • Author(s): Hori Yuki, Autio Joonas, Ohno Masahiro, Kawabata Yoshihiko, Urushibata Yuta , Murata Katsutoshi, Kawasaki Akihiro, Takeda Chiho, Yokoyama Chihiro, Yamaguchi Masataka, Yoshida Atsushi, Glasser Matthew F, Van Essen David, Hayashi Takuya
  • Organizer: 第2回ヒト脳イメージング研究会
• [Presentation] Prediction of Neurite Indices From Diffusion Tensor Imaging in the human cerebral cortex (2018)
  • Author(s): Fukutomi Hikaru, Akasaka Thai, Fujimoto Koji, Yamamoto Takayuki, Okada Tomohisa, Kaori Togashi, Hayashi Takuya
  • Organizer: ISMRM2018
  • Int'l Joint Research
• [Presentation] Does Diffusion Tensor Model Predict Neurite Distribution of Cerebral Cortical Gray Matter? ? Cortical DTI-NODDI (2018)
  • Author(s): Fukutomi Hikaru, Murata Katsutoshi, Glasser Matthew F, Akasaka Thai, Fujimoto Koji, Yamamoto Takayuki, Autio Joonas, Okada Tomohisa, Kaori Togashi, Zhang Gary Hui, Van Essen David, Hayashi Takuya
  • Organizer: 第2回ヒト脳イメージング研究会
• [Presentation] 霊長類脳コネクトームと病態解明 (2018)
  • Author(s): Hayashi Takuya
  • Organizer: 第9回札幌神経科学研究会
  • Invited
• [Presentation] Age-dependent changes of structure, myelin and neurite in adult human cerebral cortex (2018)
  • Author(s): Sasaki Akihiro, Fukutomi Hikaru, watanabe kyosuke, Morito Yusuke, Tajima Kanako, Ebisu Kyoko, Iwasaki Miho, Mizuno Kei, Hayashi Takuya, Watanabe Yasuyoshi
  • Organizer: 第2回ヒト脳イメージング研究会
• [Presentation] 霊長類脳コネクトーム (2018)
  • Author(s): 林 拓也
  • Organizer: 第20回ヒト脳機能マッピング学会
  • Invited
• [Presentation] Brain Connectome in Primates. (2018)
  • Author(s): Takuya Hayashi
  • Organizer: International Symposium of Marmoset Science
  • Int'l Joint Research / Invited
• [Presentation] Challenges of Brain Connectome in Primates (2017)
  • Author(s): Takuya Hayashi
  • Organizer: ABiS international symposium
  • Int'l Joint Research / Invited
• [Presentation] ヒト・霊長類動物脳コネクトームとパイプライン (2017)
  • Author(s): 林 拓也
  • Organizer: 第1回ヒト脳イメージング研究会
  • Invited
• [Presentation] Plastic resting-state network changes in MPTP-treated monkeys (2016)
  • Author(s): Autio Joonas, Tanki Nobuyoshi, Ose Takayuki, Takahashi Jun, Hayashi Takuya
  • Organizer: Neuroscience 2016(Society for Neuroscience, SfN)
  • Place of Presentation: San Diego Convention Center, San Diego, United States
  • Int'l Joint Research
• [Presentation] MRI・PET を用いた大脳皮質可塑性の視覚化 (2016)
  • Author(s): 林 拓也
  • Organizer: 第46回日本臨床神経生理学会
  • Place of Presentation: ホテルハマツ, 郡山
  • Invited
• [Presentation] Understanding of brain plasticity by resolving neural conenctivity dynamics using diffusion MRI (2016)
  • Author(s): Hayashi Takuya
  • Organizer: 計測自動制御学会 ライフエンジニアリング部門シンポジウム
  • Place of Presentation: 大阪国際交流センター, 大阪
  • Invited
• [Presentation] Preclinical perspectives on the mechanism of aberrant brain network and dopamine dysfunction (2016)
  • Author(s): Hayashi Takuya, Autio Joonas
  • Organizer: 国立研究開発法人国立精神・神経医療研究センター脳病態統合イメージングセンター5 周年記念国際シンポジウム
  • Place of Presentation: 国立精神・神経医療研究センター
  • Int'l Joint Research / Invited
• [Presentation] 分子コネクトームによる脳可塑性の可視化 (2016)
  • Author(s): 林 拓也
  • Organizer: 東北大学通研共同プロジェクト研究会
  • Place of Presentation: 東北大学 電気通信研究所
  • Invited