| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
Prokaryotic microorganisms play a central role in the global methane cycle for both production and consumption. Methanogenic archaea (methanogens) are a diverse group of Euryarchaeota, which grow with the enzymatic formation of methane from H2 plus CO2, acetate, and methy-compounds in anaerobic condition. Archaeal methanotrophy in sub-seafloor is also an important microbial process in the global carbon cycle and also greenhouse gas emission constraints. Since laboratory-based culture and isolation of anaerobic methanotrophic archaea (ANME) are currently difficult, the fate of sub-seafloor 13C-depleted methane emission is still unclear on their biosynthetic pathways. Cold seep ecosystems, known as a high cell density spot for modern ANME communities, is an appropriate natural laboratory for the study of methane biogeochemistry. To address the enigmatic biochemical process further, we developed the high resolution method for the relevant organic molecules of archaeal methanotrophy.
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