| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Outline of Final Research Achievements |
We have developed a fluorescent probe that can rapidly visualize hydrogen peroxide released from cells as a signal. Fluorescent probes comprise complexes, profluorophores, and linkers linking them. In this study, the complex structure, profluorophore, and linker were improved. Hydrogen peroxide could be detected even in HeLa cells which do not overexpress the EGF receptor, when the most sensitive hydrogen peroxide fluorescent probe was used. In addition, it was shown that the emission of nitrogen monoxide could be achieved in the hypoxia region of cell spheroids by using MnNO complex. The mechanism by which the complex cleaves DNA by reductive oxygen activation was also elucidated.
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