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Two-photon absorption fluorescent probes for non-invasive deep imaging

Research Project

Project/Area Number 16H04134
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional solid state chemistry
Research InstitutionYamaguchi University

Principal Investigator

Kawamata Jun  山口大学, 大学院創成科学研究科, 教授 (40214689)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords非線形光学 / 二光子吸収 / 赤外発光 / バイオイメージング / 多光子吸収 / 蛍光 / イメージング
Outline of Final Research Achievements

Two-photon fluorescence microscope is a powerful biomedical tool for obtaining three-dimensional images of tissues and organs. The use of far-red/near-infrared light (above 650 nm) offers the advantages of minimal auto-fluorescence and/or absorption by endogenous molecules in tissues, as well as reduced light scattering because of the longer wavelengths. This results in large penetration depth, thus enabling deep in vivo 3D imaging. In this project, several far-red fluorescent probes with efficient two-photon excitation sensitivities have been designed. Two-photon absorption wavelengths of the probes were tuned to be 1030 nm which is a radiation wavelength of a stable and low cost light source, a femtosecond pulsed Yb-doped fiber laser. These novel probes should realize non-invasive deep imaging.

Academic Significance and Societal Importance of the Research Achievements

二光子励起顕微鏡は、生体の営みを動的かつ三次元的に可視化できる装置として注目を集めている。しかし、1億円以上もする高価な機器であるため、共同利用機器として総合大学が導入しているに過ぎない。この装置が高価な理由は、現在使われている蛍光プローブの励起光源に、高価なフェムト秒チタンサファイアレーザーが必要なことにある。本研究で開発された蛍光プローブにより、低コストで取扱が簡単なフェムト秒ファイバーレーザーを光源にした二光子励起イメージングが実現した。この成果は、二光子励起顕微鏡のコスト低下、汎用性の向上を通じて、医学や生物学の研究の発展に貢献できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (26 results)

All 2018 2017 2016 Other

All Int'l Joint Research (5 results) Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 4 results) Remarks (2 results)

  • [Int'l Joint Research] コメニウス大学(スロバキア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] トリプラ大学/ボパール工科大学(インド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] バイロイト大学(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] コメニウス大学(スロバキア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] トリプラ大学(インド)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Bright and two-photon active red fluorescent dyes that selectively move back and forth between the mitochondria and nucleus upon changing the mitochondrial membrane potential2018

    • Author(s)
      Seki Hitomi、Onishi Shozo、Asamura Naoya、Suzuki Yasutaka、Kawamata Jun、Kaneno Daisuke、Hadano Shingo、Watanabe Shigeru、Niko Yosuke
    • Journal Title

      Journal of Materials Chemistry B

      Volume: 6 Issue: 45 Pages: 7396-7401

    • DOI

      10.1039/c8tb02415d

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrion-selective hemicyanine dyes suitable for fiber laser excitation two-photon microscopy2018

    • Author(s)
      Moritomo H.、Onishi S.、Asamura N.、Matsumoto K.、Suzuki Y.、Kawamata J.
    • Journal Title

      MRS Communications

      Volume: 8 Issue: 3 Pages: 1064-1069

    • DOI

      10.1557/mrc.2018.143

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two-photon absorption spectrum of solid solution nanocrystals of ZnS-AgInS22018

    • Author(s)
      Yasutaka Suzuki, Shozo Onishi, Kazuya Matsumoto, Takayuki Takiyama, Tatsuya Kameyama, Tsukasa Torimoto, and Jun Kawamata
    • Journal Title

      Chem. Lett.

      Volume: 47 Pages: 1475-1477

    • DOI

      10.1246/cl.180718

    • NAID

      130007534800

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The effect of the negative charge density on switchable properties of pseudoisocyanine dye2017

    • Author(s)
      Matejdes Marian、Himeno Daisuke、Suzuki Yasutaka、Kawamata Jun
    • Journal Title

      Applied Clay Science

      Volume: 144 Pages: 54-59

    • DOI

      10.1016/j.clay.2017.05.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The emergent intramolecular hydrogen bonding effect on the electronic structures of organic electron acceptors2017

    • Author(s)
      Takashi Takeda, Yasutaka Suzuki, Jun Kawamata, Shin-ichiro Noro, Takayoshi Nakamura, Tomoyuki Akutagawa
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 19 Issue: 35 Pages: 23905-23909

    • DOI

      10.1039/c7cp04402j

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biological oxygen sensing via two-photon absorption by an Ir(III) complex using a femtosecond fiber laser2016

    • Author(s)
      Morimoto H, Fujii A, Suzuki Y, Yoshihara T, Tobita S, Kawamata J
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 55 Issue: 9 Pages: 092401-092403

    • DOI

      10.7567/jjap.55.092401

    • NAID

      210000147062

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Red Fluorescent Compounds for Fiber Laser Excitation Multi-photon Imaging2018

    • Author(s)
      Syozo Onishi, Yasutaka Suzuki, Jun Kawamata
    • Organizer
      The 3rd Taiwan-Japan Workshop on Nanospace Materials
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optical Properties of Dyes Confined in Interlayer Spaces of Clay Minerals2018

    • Author(s)
      Jun Kawamata
    • Organizer
      The 3rd Taiwan-Japan Workshop on Nanospace Materials
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ファイバーレーザー励起による多光子イメージングに適した蛍光化合物の開発2018

    • Author(s)
      大西省三、松本和也、守友博紀、鈴木康孝、川俣純
    • Organizer
      日本化学会「低次元系光機能材料研究会」第8回サマーセミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] Mitochondrial Membrane Potential Sensitive Red-Green-Blue Fluorescent Probes for Multi-Photon Microscope2018

    • Author(s)
      Shozo Onishi, Naoya Asamura, Hitomi Seki, Yosuke Niko, Seiji, Jun Kawamata, Yasutaka Suzuki
    • Organizer
      MRS spring meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluorescent Compounds for Fiber-Laser Excitation Multi-Photon Imaging2018

    • Author(s)
      Jun Kawamata, Yasutaka Suzuki
    • Organizer
      MRS spring meeting
    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シアニン誘導体を用いたファイバーレーザーによる二光子励起イメージング2018

    • Author(s)
      大西省三、 松本和也、 浅村直哉、 守友博紀、 鈴木康孝、 川俣純
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ピリジニウム誘導体をプローブとしたフェムト秒ファイバーレーザー励起多光子蛍光イメージング2017

    • Author(s)
      守友博紀、 牧野洋平、 大村悠理子、 浅村直哉、 松下寛史、 大西省三、 鈴木康孝、 川俣純
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶應義塾大学(神奈川県・横浜市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Fiber laser excitable fluorescent compounds for multi-photon imaging2017

    • Author(s)
      Jun Kawamata
    • Organizer
      Second International Conference on MATERIAL SCIENCE
    • Place of Presentation
      トリプラ(インド)
    • Year and Date
      2017-02-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ミトコンドリア染色用赤色発光性化合物の多光子励起挙動2017

    • Author(s)
      大西省三、 富永亮、 鈴木康孝、 川俣純、 仁子陽輔、 小西玄一
    • Organizer
      2017年光化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミトコンドリア膜電位応答性を示す赤色蛍光プローブ2017

    • Author(s)
      浅村直哉、 大西省三、 関仁望、 仁子陽輔、 鈴木康孝、 川俣純
    • Organizer
      日本化学会中国四国支部大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 赤色発光性ピレン誘導体の二光子励起特性2017

    • Author(s)
      大西省三、 富永亮、 鈴木康孝、 川俣純、 仁子陽輔、 小西玄一
    • Organizer
      日本化学会「低次元系光機能材料研究会」第6回サマーセミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] フェムト秒ファイバーレーザーを光源としたマルチフォトンイメージング2016

    • Author(s)
      川俣純
    • Organizer
      第13回バイオオプティクス研究会
    • Place of Presentation
      前橋工科大学(群馬県・前橋市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] フェムト秒ファイバーレーザーを光源とした多光子励起蛍光イメージング2016

    • Author(s)
      牧野洋平、守友博紀、鈴木康孝、川俣純
    • Organizer
      物質構造解析研究会
    • Place of Presentation
      山口大学(山口県・山口市)
    • Related Report
      2016 Annual Research Report
  • [Remarks] 山口大学 理学部 物性化学研究室

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~kawalab/

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] 山口大学理学部 物性化学研究室

    • URL

      http://web.cc.yamaguchi-u.ac.jp/~kawalab/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-07-01  

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