| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Outline of Final Research Achievements |
Two-photon fluorescence microscope is a powerful biomedical tool for obtaining three-dimensional images of tissues and organs. The use of far-red/near-infrared light (above 650 nm) offers the advantages of minimal auto-fluorescence and/or absorption by endogenous molecules in tissues, as well as reduced light scattering because of the longer wavelengths. This results in large penetration depth, thus enabling deep in vivo 3D imaging. In this project, several far-red fluorescent probes with efficient two-photon excitation sensitivities have been designed. Two-photon absorption wavelengths of the probes were tuned to be 1030 nm which is a radiation wavelength of a stable and low cost light source, a femtosecond pulsed Yb-doped fiber laser. These novel probes should realize non-invasive deep imaging.
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