| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
We recently proposed a new strategy for analyzing the siRNA delivery process based on affinity labeling with a peptide nucleic acid (PNA)-based fluorescent probe capable of selectively binding to the overhanging structures of siRNAs (Chem. Commun. 2015). Here, we have successfully prepared a new probe with improved binding affinity and imaging ability by conjugation with a cationic oligopeptide (ChemBioChem 2019). In addition, we have developed new fluorogenic sensing probes for double-stranded RNA (dsRNA) by integrating thiazole orange (TO) as a base surrogate into triplex-forming PNA (J. Am. Chem. Soc. 2016; Chem. Eur. J. 2017; Org . Biomol. Chem. 2017; Org . Biomol. Chem. 2018; RSC Advances 2018). Furthermore, we have developed useful fluorescent indicators for the assessment of the binding capabilities of various test compounds for the internal loop structure of the bacterial ribosomal decoding region of the aminoacyl-tRNA site (A-site) (Chem. Eur. J. 2018; Chem. Commun. 2019).
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