• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of microfluidic vascular models to study vascular function

Research Project

Project/Area Number 16H04170
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionJapan Women's University

Principal Investigator

SATO Kae  日本女子大学, 理学部, 教授 (40373310)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywordsマイクロデバイス / 血管 / 血液細胞 / マイクロ流路分析 / 造血幹細胞 / 肺高血圧症 / マイクロ・ナノデバイス / 細胞・組織 / 分析科学 / 生体機能利用
Outline of Final Research Achievements

In this study, we developed microdevices for use in vascular bioassays. We have developed a device that mimics blood flow and can be used for fluid culture, a cell-stretching device that mimics vasodilation, and a device made of gelatin. By changing the cells to be cultured in these devices, various vascular models can be created. Applications include: 1) hematopoiesis during early mammalian development, and 2) smooth muscle cell proliferation in pulmonary hypertension.

Academic Significance and Societal Importance of the Research Achievements

細胞バイオアッセイは、動物を使わずに物質が生物に及ぼす影響を細胞で評価する分析方法である。近年、ヒトの細胞微小環境を正確に反映する組織モデルによるアッセイの開発が求められている。本研究では、血管内皮細胞の培養条件を詳細に調べ周囲の細胞や間質とともに再構成することで、生理的に適切な血管モデルの構築を試みた。開発したモデルは発生学から難治疾患解明まで広く血管研究に貢献できる。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (13 results)

All 2022 2021 2019 2018 2017

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (8 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] マイクロ血管デバイスの開発とバイオ分析化学への応用2022

    • Author(s)
      佐藤香枝
    • Journal Title

      分析化学

      Volume: 71 Pages: 53-58

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Influence of Culture Conditions on Cell Proliferation in a Microfluidic Channel2019

    • Author(s)
      SATO Kae、SATO Miwa、YOKOYAMA Mizuho、HIRAI Mai、FURUTA Aya
    • Journal Title

      Analytical Sciences

      Volume: 35 Issue: 1 Pages: 49-56

    • DOI

      10.2116/analsci.18SDP04

    • NAID

      130007554818

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2019-01-10
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A microfluidic cell stretch device to investigate the effects of stretching stress on artery smooth muscle cell proliferation in pulmonary arterial hypertension.2019

    • Author(s)
      Sato K, Nitta M, Ogawa A.
    • Journal Title

      Inventions

      Volume: 4 Issue: 1 Pages: 1-1

    • DOI

      10.3390/inventions4010001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent Progress in the Development of Microfluidic Vascular Models2018

    • Author(s)
      SATO Kae、SATO Kiichi
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 7 Pages: 755-764

    • DOI

      10.2116/analsci.17R006

    • NAID

      130007403436

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2018-07-10
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a Simple Permeability Assay Method for Snake Venom-induced Vascular Damage2018

    • Author(s)
      SATO Kae、KODAMA Ayuki、KASE Chikako、HIRAKAWA Satoshi、ATO Manabu
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 3 Pages: 323-327

    • DOI

      10.2116/analsci.34.323

    • NAID

      130006470875

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ゼラチンを用いた3ウェル連結型細胞培養デバイスの作製2021

    • Author(s)
      池田 涼音、関根 詩乃、別所 朋香、大月 陽香、柴田 紗希、中野 実紅、佐藤 香枝
    • Organizer
      化学とマイクロ・ナノシステム学会 第43回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 伸展培養したOP9細胞が血液細胞分化に及ぼす影響:伸展培養で活性化される遺伝子の解明2021

    • Author(s)
      池田 涼音,前田 桃子,北島 健二,原 孝彦,佐藤 香枝
    • Organizer
      Chem-Bio Joint Seminar 2021
    • Related Report
      2019 Annual Research Report
  • [Presentation] 共焦点顕微鏡で観察可能な再剥離式細胞培養デバイスの作製2021

    • Author(s)
      関根 詩乃,佐藤 香枝
    • Organizer
      Chem-Bio Joint Seminar 2021
    • Related Report
      2019 Annual Research Report
  • [Presentation] DEVELOPMENT OF CELL CULTURE MICRODEVICE USING GELATIN GEL2019

    • Author(s)
      Sasaki, S., Sato, K
    • Organizer
      The 23rd International Conference on. Miniaturized Systems for Chemistry and Life Sciences (microTAS 2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] STRETCHABLE MICROPATTERNED MEMBRANE INTEGRATED IN MICROFLUIDIC DEVICES FOR PULMONARY ARTERY SMOOTH MUSCLE CELL CULTURE2018

    • Author(s)
      Kae Sato, Yuka Misawa, Kyojiro Morikawa, Kazuma Mawatari, Takehiko Kitamori
    • Organizer
      microTAS 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生体環境を模倣した血管マイクロ実験室の構築2017

    • Author(s)
      佐藤香枝
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Development of cell stretching device for pulmonary hypertension studies2017

    • Author(s)
      Manami Nitta, Aiko Ogawa, and Kae Sato
    • Organizer
      MiroTAS2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mechanical stress-induced blood cell production in a microfluidic device2017

    • Author(s)
      Eriko Kamata, Kanako Yanagisawa, Kenji Kitajima, Takahiko Hara, and Kae Sato
    • Organizer
      MiroTAS2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2016-04-21   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi