Development of ddPCR platform utilizing specific properties of PDMS microfluidic device
Project/Area Number |
16H04171
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Analytical chemistry
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Research Institution | Doshisha University |
Principal Investigator |
|
Research Collaborator |
Nakashoji Yuta
Murata Yuki
Tanaka Yugo
Toda Miwako
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
|
Keywords | マイクロフルイディクス / ドロップレット / エマルション / マイクロ流体デバイス / デジタルPCR / ポリジメチルシロキサン / ピエゾマイクロポンプ / PDMS / 分析科学 / ドロップレットデジタルPCR / 癌 / マイクロ・ナノデバイス / バイオ関連機器 / ゲノム |
Outline of Final Research Achievements |
In this study, the principle investigator successfully developed a microfluidic device that allowed to produce monodisperse water-in-oil droplets automatically and reproducibly utilizing the high gas solubility feature of polydimethylsiloxane. Furthermore, with droplet PCR experiments using the droplets obtained by the above device, it was verified that the initial DNA concentration of each sample predicted using the fraction of fluorescence positive droplets on the basis of the Poisson distribution was well concordant with the preset sample concentration. In addition to the studies described above, the principle investigator exploited a miniaturized fluid control system using a piezoelectric micropump for high-throughput flow-through screening of droplets. It was validated that the current system could screen droplets at a rate of 120 droplets per second. Thus, usability of the developed flow control system for the high-throughput droplet monitoring was demonstrated successfully.
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Academic Significance and Societal Importance of the Research Achievements |
ドロップレット・デジタルPCR(ddPCR)は、これまで開発された方法では実現成し得なかった遺伝子診断を可能とするため大きな脚光を浴びている。しかし、既存のddPCRプラットフォームは、システム全体が大きい・取り扱いが煩雑・コスト高であるといった問題点が指摘されている。本研究により、少ない初期投資費用で取り扱い易く、小型のddPCRプラットフォームが構築された。本研究によって、ddPCR研究へ新規参入する研究者が増加し、ddPCR研究の開発スピードが加速していくものと期待される。
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Report
(4 results)
Research Products
(21 results)