Development of a novel nuclear resonance vibrational spectrometer to elucidate the mechanism in the active center of iron enzymes
| Project/Area Number |
16H04172
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Japan Synchrotron Radiation Research Institute |
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Principal Investigator |
Yoda Yoshitaka 公益財団法人高輝度光科学研究センター, 利用研究促進部門, 主幹研究員 (90240366)
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| Research Collaborator |
Cramer Stephen P.
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | 量子ビーム / 酵素反応 / 核共鳴振動分光 / 核共鳴非弾性散乱 / 核共鳴散乱 / 生物化学 / 高分解能モノクロメータ / ヒドロゲナーゼ / 酵素 / 鉄酵素 / 放射光 / Ｘ線光学 |
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| Outline of Final Research Achievements |
Nuclear resonance vibrational spectroscopy is a powerful tool to investigate the active center of the iron metalloenzymes because it selectively probe the vibrational motion of Fe-57. But the observable vibrational modes were limited due to the lower resolution or the lower counting rates of the signals. We have developed a novel nuclear resonance vibrational spectrometer and observed the iron-hydrogen bending mode in hydrogenase which reversibly catalyze the molecular hydrogen. It contributes to the deep understanding of the mechanism of the hydrogenase.
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| Academic Significance and Societal Importance of the Research Achievements |
活性中心に金属をもつ金属酵素のなかで鉄酵素は水素を酸化還元するヒドロゲナーゼをはじめ、エネルギー問題・食糧問題・医療などと密接に関わっているものが多い。すでに結果も出始めているが、開発した核共鳴振動分光装置の利用による鉄酵素の活性機構の解明を通じて、タンパク質の動的機能の理解を深めることができる。一方、得られた知見は大きな投資が行われている水素と酸素を水に変換する燃料電池電極を始めとする人工触媒の開発において重要なヒントを与えると期待されている。
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Impurity position and lattice distortion in a Mn-doped Bi2Te3 topological insulator investigated by x-ray fluorescence holography and x-ray absorption fine structure2017
Author(s)
S. Hosokawa, J. R. Stellhorn, T. Matsushita, N. Happo, K. Kimura, K. Hayashi, Y. Ebisu, T. Ozaki, H. Ikemoto, H. Setoyama, T. Okajima, Y. Yoda, H. Ishii, Y.-F. Liao, M. Kitaura, and M. Sasaki
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Journal Title
Physical Review B
Volume: 96
Issue: 21
Pages: 214207-214207
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Evolution of synchrotron-radiation-based Mossbauer absorption spectroscopy for various isotopes2017
Author(s)
Makoto Seto, Ryo Masuda, Yasuhiro Kobayashi, Shinji Kitao, Masayuki Kurokuzu, Makina Saito, Shuuich Hosokawa, Hiroki Ishibashi, Takaya Mitsui, Yoshitaka Yoda, Ko Mibu
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Journal Title
Hyperfine Interactions
Volume: 238
Issue: 1
Pages: 78-78
DOI
Related Report
Peer Reviewed
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