| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
Cell culture technology is an important technology in cell therapy such as regenerative medicine. In this study, we aimed to establish the basic technology of automated cell culture system using ultrasonic technology.
First, it was clarified that cells attached to the bottom of culture dish can be detached without using any proteinase by applying ultrasonic vibration to culture dish. Next, ultrasonic pumping was applied to establish a method to recover the cell suspension in the culture dish by placing a glass tube close to the vibrating culture surface. Furthermore, by applying ultrasonic waves with appropriate amplitude distribution to culture dishes, we established a technology to pattern cells on a culture substrate.
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