Dynamics of Morphological Changes of Cells and Its High-Accuracy Kinetic Model under High Temperature Related to Thermal Therapy
Project/Area Number |
16H04281
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Thermal engineering
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Research Institution | Kyushu Institute of Technology |
Principal Investigator |
ISHIGURO Hiroshi 九州工業大学, 大学院生命体工学研究科, 教授 (30176177)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Keywords | 熱工学 / バイオ熱工学 / 温度環境 / 細胞挙動 / 形態変化 / ダイナミクス / 数学モデル / 逆問題解析 / 細胞動態 / 高温環境 / 死滅 |
Outline of Final Research Achievements |
Morphological changes as one of fundamental characteristics of adherent cultured cells were investigated by time-lapse 3D imaging using digital holographic microscopy to understand the dynamics of morphological changes under the higher-temperature stress (40.0-47.5°C) than 37.0°C. Next, a high-accuracy kinetic model was proposed and developed to describe mathematically the process through the characteristic morphological changes of cells to the morphological collapse. In addition, the protective effect of antioxidants on the collapse of cells (cell death) was found out. Also, the above similar characteristics: the dynamics of morphological changes, the mathematical model and the effect of antioxidants, under the lower-temperature stress (35.0-2.5°C) than 37.0°C were made clear.
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Academic Significance and Societal Importance of the Research Achievements |
細胞の基本的特性の一つである形態変化のダイナミクスについての詳細な理解は、熱生物学的特性という基礎的観点から大変興味深いと共に、機序の解明、数学的モデル化、関連プロセスや機器の設計・最適化などの点から重要で、医療応用(応用範囲・適用条件の拡大、高精度化と信頼性の向上)に資する。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] A PalmSized Cryoprobe System with a Builtin Thermocouple and Its Application in an Animal Model of Epilepsy2019
Author(s)
Tokiwa T, Zimin L, Ishiguro, H, Inoue, T, Kajigaya, H, Nomura, S, Suzuki, M, and Yamakawa, T.
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Journal Title
IEEE Engineering in Medicine and Biological Society, IEEE
Volume: 印刷中
Issue: 11
Pages: 3168-3175
DOI
Related Report
Peer Reviewed
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