| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Morphological changes as one of fundamental characteristics of adherent cultured cells were investigated by time-lapse 3D imaging using digital holographic microscopy to understand the dynamics of morphological changes under the higher-temperature stress (40.0-47.5°C) than 37.0°C. Next, a high-accuracy kinetic model was proposed and developed to describe mathematically the process through the characteristic morphological changes of cells to the morphological collapse. In addition, the protective effect of antioxidants on the collapse of cells (cell death) was found out. Also, the above similar characteristics: the dynamics of morphological changes, the mathematical model and the effect of antioxidants, under the lower-temperature stress (35.0-2.5°C) than 37.0°C were made clear.
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