| Budget Amount *help |
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
In this research project, as an on-chip cell measurement system integrating top-down and bottom-up approach, 1) Manipulation and cell injection of single fluorescence microsensor using multiple wavelength lights of 1064 nm and 808 nm as bottom-up cell measurement, 2) Long-term fluorescence measurement using hydrogel fluorescence microsensor by diffusion phenomenon of fluorescent dye in hydrogel as a top-down approach of cell measurement, were achieved.
We established the basic technologies for on-chip cell measurement using an optical measurement method, such as rapid and selective cell injection of fluorescence microsensor for intracellular measurement and extremely long measurement more than 1000 times using hydrogel fluorescence microsensor with fluorescence recovery in the sensor.
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