Application of hybrid nano-assembly for in-vitro synthetic biology tool
| Project/Area Number |
16H04570
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tohoku University |
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Principal Investigator |
Umetsu Mitsuo 東北大学, 工学研究科, 教授 (70333846)
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| Co-Investigator(Kenkyū-buntansha) |
二井手 哲平 東北大学, 工学研究科, 助教 (20802705)
高見 誠一 名古屋大学, 工学研究科, 教授 (40311550)
中澤 光 東北大学, 工学研究科, 助教 (40584991)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | 生体分子 / 酵素 / ナノ粒子 / ナノ材料 / 生物・生体工学 / 酵素反応 |
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| Outline of Final Research Achievements |
In vivo, a series of enzymes are closely packed in a minute space, and the enzyme reactions are coupled. In this study, enzymes are clustered on nanoparticles and the clustered enzymes are hierarchically assembled to reconstruct in-vivo-mimic enzyme-crowded environment. The utilization of material-binding peptides and proteins showed the possibility for spontaneous self-assembly of clustered enzymes.
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| Academic Significance and Societal Importance of the Research Achievements |
生体内での酵素密集環境を生体外で再構築できれば、細胞内では変化させることができなかった様々なパラメータを変化させることができ、生体内での代謝ネットワークの解明に有用なツールとなり得る。本研究で開発した、酵素が集積したナノ粒子を凝集させる技術は、その再構築を可能とする要素技術として寄与する。
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Compact seahorse-shaped T cell-activating antibody for cancer therapy2018
Author(s)
Hiroto Fujii, Yoshikazu Tanaka, Hikaru Nakazawa, Aruto Sugiyama, Noriyoshi Manabe, Akira Shinoda, Nobutaka Shimizu, Takamitsu Hattori, Katsuhiro Hosokawa, Takuma Sujino, Tomoyuki Ito, Teppei Niide, Ryutaro Asano, Izumi Kumagai, and Mitsuo Umetsu
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Journal Title
Advanced Therapeutics
Volume: 1700031
Issue: 3
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing2017
Author(s)
Yuki Shibuya, Natsuki Haga, Ryutaro Asano, Hikaru Nakazawa, Takamitsu Hattori, Daisuke Takeda Aruto Sugiyama, Reiko Kurotani, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe
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Journal Title
Protein Engineering, Design & Selection
Volume: 30
Pages: 15-21
DOI
Related Report
Peer Reviewed
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