| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
It was shown that chicken IFITM10 slightly reduced the infectivity of VSV-G-pseudotyped virus. In addition, overexpression of Muc13, of which gene size is below the limit to construct an expression vector, did not prevent infectivity of VSV-G-pseudotyped virus. Thus, expression system for large size mucin is under construction.
We examined the gene transfer and genome editing conditions suitable for the long-term cultured primordial germ cells. Using CRISPR/Cas9, we succeeded in producing a genome-edited chicken in which the EGFP gene was knocked in.
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