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Molecular-level understanding of the effects of microorganisms on migration of radionuclides

Research Project

Project/Area Number 16H04634
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nuclear engineering
Research InstitutionKyushu University

Principal Investigator

Utsunomiya Satoshi  九州大学, 理学研究院, 准教授 (40452792)

Co-Investigator(Kenkyū-buntansha) 大貫 敏彦  東京工業大学, 科学技術創成研究院, 教授 (20354904)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2016: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywordsアクチノイド / 微生物 / ナノ粒子 / ランタノイド / 細胞外放出物 / 地圏微生物 / 環境ナノ粒子 / 放射性核種 / コロイド移行 / 糖分子 / コロイド / EPS / Colloid stabiilty / ナノ材料 / 原子力エネルギー / ナノバイオ
Outline of Final Research Achievements

This project aimed to understand interaction between actinides nanoparticles - microorganisms for a possible implication to actinides migration surrounding deep underground repository. We have elucidated the nanocrystallization processes of lanthanides (surrogate of actinides) phosphate in the P-free initial solution, which was released with extracellular polymeric substances (EPS)from microorganisms (S. Cerevisiae). Colloidal stability of Ln oxides nanoparticles was enhanced by EPS adsorbed to the nanoparticles. In addition, Ln oxides nanoparticles were experimentally exposed to S. Cerevisiae. The proportion of extra-cellular polymeric substances changes by the presence of nanoparticles and a protein involved in glycolysis system. The results indicate that migration of actinides mediated by biogenic and natural nanoparticles is constrained by the complex interaction between microorganisms and nanoparticles.

Academic Significance and Societal Importance of the Research Achievements

本研究は、放射性核種―微生物間相互作用で核種移行に対する影響が大きい核種ナノ結晶生成に注目し、その精密な特性評価と核種ナノ結晶―微生物間相互作用の理解を分子レベルで確立した。特に、ナノ粒子が微生物に与える影響を発現タンパク質の解析による代謝経路への影響評価まで深め、逆に微生物がナノ粒子に与える影響という双方向の作用に対して系統的にEPSの影響を網羅して、放射化学―地球化学―生物化学の分野融合によって柔軟に新しい成果を出すことに成功している。また、本研究はバックエンド分野において、核種移行に対する微生物影響因子に重要な知見を与え、世界レベルの研究・教育拠点として若い原子力人材の育成に貢献した。

Report

(5 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (16 results)

All 2021 2020 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Virginia Polytech Institute(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Virginia Polytechnical Institute(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Virginia Polytech(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] The role of nanoscale aggregation of ferrihydrite and amorphous silica in the natural attenuation of contaminant metals at mill tailings sites2021

    • Author(s)
      Kawamoto Keisuke、Yokoo Hiroki、Ochiai Asumi、Nakano Yuriko、Takeda Ayaka、Oki Takumi、Takehara Masato、Uehara Motoki、Fukuyama Kenjin、Ohara Yoshiyuki、Ohnuki Toshihiko、Hochella Michael F.、Utsunomiya Satoshi
    • Journal Title

      Geochimica et Cosmochimica Acta

      Volume: 298 Pages: 207-226

    • DOI

      10.1016/j.gca.2021.02.004

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Application of Electron Microscopy to Understanding Colloid-Facilitated Transport of Radionuclides at the Mayak Production Association Facility, Near Lake Karachai, Russia2020

    • Author(s)
      Utsunomiya Satoshi、Yokoo Hiroki、Oki Takumi、Kawamoto Keisuke、Takeda Ayaka、Wang Honggui、Ewing Rodney C.
    • Journal Title

      Behavior of Radionuclides in the Environment

      Volume: I Pages: 177-200

    • DOI

      10.1007/978-981-15-0679-6_7

    • ISBN
      9789811506789, 9789811506796
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of Microbes on Chemical Species Transformation of Radionuclides2020

    • Author(s)
      Ohnuki Toshihiko、Ozaki Takuo、Kozai Naofumi、Utsunomiya Satoshi
    • Journal Title

      Behavior of Radionuclides in the Environment

      Volume: I Pages: 67-92

    • DOI

      10.1007/978-981-15-0679-6_3

    • ISBN
      9789811506789, 9789811506796
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Commentary on the Role of Microorgansims and Nanoparticles in Radionuclides Migration through Groundwater2020

    • Author(s)
      Utsunomiya Satoshi、Ohnuki Toshihiko、Kato Kenji、Kalmykov Stepan N.
    • Journal Title

      Behavior of Radionuclides in the Environment

      Volume: 1 Pages: 221-225

    • DOI

      10.1007/978-981-15-0679-6_9

    • ISBN
      9789811506789, 9789811506796
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The competing effects of microbially derived polymeric and low molecular-weight substances on the dispersibility of CeO2 nanoparticles.2018

    • Author(s)
      Nakano Y, Ochiai A, Kawamoto K, Takeda A, Ichiyoshi K, Ohnuki T, Hochella MF, Utsunomiya S
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 3648-3648

    • DOI

      10.1038/s41598-018-21976-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Adsorption of Extracellular Polymeric Substances Derived from S. cerevisiae to Ceria Nanoparticles and the Effects on Their Colloidal Stability2017

    • Author(s)
      Masaki Shota、Nakano Yuriko、Ichiyoshi Kenta、Kawamoto Keisuke、Takeda Ayaka、Ohnuki Toshihiko、Hochella, Jr. Michael、Utsunomiya Satoshi
    • Journal Title

      Environments

      Volume: 4 Issue: 3 Pages: 48-48

    • DOI

      10.3390/environments4030048

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystal Chemistry and Stability of Hydrated Rare-Earth Phosphates Formed at Room Temperature2017

    • Author(s)
      Asumi Ochiai、Satoshi Utsunomiya
    • Journal Title

      Minerals

      Volume: 7 Issue: 5 Pages: 84-84

    • DOI

      10.3390/min7050084

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Removal of soluble strontium via incorporation into biogenic carbonate minerals by halophilic bacterium Bacillus sp. strain TK2d in a highly saline solution.2017

    • Author(s)
      Horiike T, Dotsuta Y, Nakano Y, Ochiai A, Utsunomiya S, Ohnuki T, Yamashita M
    • Journal Title

      Appliedl Environmental Microbiology

      Volume: 83 Issue: 20

    • DOI

      10.1128/aem.00855-17

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Effects of extracellular polymeric substances on the aggregation of CeO2 nanoparticles2017

    • Author(s)
      Yuriko Nakano, Satoshi Utsunomiya
    • Organizer
      Goldschmidt conference
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nanocrystallization of rare earth phosphate using extracellular substnances derived from Saccharomyces cerevisiae2017

    • Author(s)
      Kenta Ichiyoshi, Asumi Ochiai, Satoshi Utsunomiya
    • Organizer
      Goldschmidt conference
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effects of organic molecules on the aggregation of CeO2 nanoparticles2016

    • Author(s)
      Yuriko Nakano, Shota Masaki, and Satoshi Utsunomiya
    • Organizer
      Goldschmidt conference
    • Related Report
      2016 Annual Research Report
  • [Presentation] 放射性核種移行挙動に対する微生物影響因子の分子科学的研究 (2) 有機分子の吸着によるナノ粒子の凝集機構への影響評価2016

    • Author(s)
      中野友里子 宇都宮聡
    • Organizer
      原子力学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 放射性核種移行挙動に対する微生物影響因子の分子科学的研究 (3)Saccharomyces cerevisiae由来の細胞外放出物と希土類元素間の相互作用2016

    • Author(s)
      市吉健太 落合朝須美 宇都宮聡
    • Organizer
      原子力学会
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2022-02-21  

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