Imaging analysis of synaptic vesicle functions using fluorescent probes
| Project/Area Number |
16H04675
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Research Collaborator |
Mori Yasunori
Egashira Yoshihiro
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | シナプス小胞 / 神経伝達物質・受容体 / シナプス伝達 / 蛍光イメージング |
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| Outline of Final Research Achievements |
Neurotransmitter release from the presynaptic terminals is elicited by the exocytosis of synaptic vesicles. However, it has been challenging to understand underlying molecular mechanisms due to the limited technologies to track them in vivo. We have applied pH-sensitive fluorescent proteins placed in the lumen of synaptic vesicles in cultured neurons derived from rodent hippocampus, and found various novel aspects of molecular physiology of presynaptic terminals, including the distinct molecular mechanisms differentiating uptake of the excitatory and the inhibitory neurotransmitters, proteins responsible for Ca2+ homeostasis and a unique SNARE protein responsible for activity-dependent mobilization of synaptic vesicles.
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| Academic Significance and Societal Importance of the Research Achievements |
脳内の情報伝達はシナプスという特徴的な細胞接着構造で行われており、シナプスでの情報伝達の破綻は、てんかんや自閉症、統合失調症などの脳疾患の原因となることがわかってきた。一方で、シナプス伝達の仕組みや、それを支える個々の分子の働きについては不明な点が多い。今回の我々の研究成果は、シナプス伝達の仕組みを理解する上で有用な知見の一端であり、将来的には、脳疾患とその治療を考える上で基盤となる可能性を秘めている。
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Report
(4 results)
Research Products
(22 results)
