| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Neurotransmitter release from the presynaptic terminals is elicited by the exocytosis of synaptic vesicles. However, it has been challenging to understand underlying molecular mechanisms due to the limited technologies to track them in vivo. We have applied pH-sensitive fluorescent proteins placed in the lumen of synaptic vesicles in cultured neurons derived from rodent hippocampus, and found various novel aspects of molecular physiology of presynaptic terminals, including the distinct molecular mechanisms differentiating uptake of the excitatory and the inhibitory neurotransmitters, proteins responsible for Ca2+ homeostasis and a unique SNARE protein responsible for activity-dependent mobilization of synaptic vesicles.
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