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Reverse genetic studies on mitochondrial DNA with pathogenic mutations in mice

Research Project

Project/Area Number 16H04678
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionUniversity of Tsukuba

Principal Investigator

NAKADA Kazuto  筑波大学, 生命環境系, 教授 (80323244)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsミトコンドリア / ミトコンドリアゲノム / 突然変異 / エネルギー代謝 / ミトコンドリア病 / モデルマウス / ミトコンドリアDNA / ミトコンドリア遺伝子疾患 / 病態モデルマウス / ミトコンドリアゲノム変異 / エネルギー産生不全 / エネルギー産生異常 / 病原性突然変異 / エネルギー代謝異常
Outline of Final Research Achievements

It has been suggested that a predominant accumulation of mutated mitochondrial DNA (mtDNA) in affected cells and tissues is responsible for multiple disorders, such as mitochondrial diseases, diabetes, neurodegerative diseases, and cancer. However, little is known about a precise pathogenesis for understanding how mutated mitochondrial DNAs induce these multiple disorders. In this work, I have succeeded in generating a novel model mouse harboring a point mutation in tRNA-Lue(UUR). In human cases, mtDNA with a point mutation in tRNA-Lue(UUR) is frequently observed in mitochondrial diseases and diabetes, so that the novel model mouse generated in this work would be an important for understanding mtDNA-based disorders. Moreover, this work showed mitochondrial fission and diabetic vital condition could regulate pathogenicity and accumulation dynamics of mutated mtDNAs in affected tissues.

Academic Significance and Societal Importance of the Research Achievements

mtDNAの突然変異が多様な病気の原因になるのか?という根源的な問いに答えるには、変異型mtDNAを導入したモデルマウスの作製が極めて有効な研究戦略となる。しかし、二重の生体膜に完全に閉ざされたミトコンドリアマトリックスに、それも複数コピー存在するmtDNAに人為的な突然変異を導入することは不可能である。本研究では、変異型mtDNAを含有するミトコンドリアをそのままES細胞に導入することで変異型mtDNAを有するマウスを作製し、さらにこれらを活用することで、変異型mtDNAの病原性制御や多様な病態発症との関連を解明した。このmtDNA変異のマウス逆遺伝学的な解析自体が学術的な価値となる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (20 results)

All 2019 2018 2017 2016 Other

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 7 results) Remarks (3 results)

  • [Journal Article] Concentration of mitochondrial DNA mutations by cytoplasmic transfer from platelets to cultured mouse cells.2019

    • Author(s)
      Ishikawa K, Kobayashi K, Yamada A, Umehara M, Oka T, Nakada K.
    • Journal Title

      PLoS ONE

      Volume: 14 Issue: 3 Pages: 0213283-0213283

    • DOI

      10.1371/journal.pone.0213283

    • NAID

      120007133505

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acquired expression of mutant Mitofusin 2 causes progressive neurodegeneration and abnormal behavior.2019

    • Author(s)
      Ishikawa K, Yamamoto S, Hattori S, Nishimura N, Tani H, Mito T, Matsumoto H, Miyakawa T, Nakada K.
    • Journal Title

      J Neurosci

      Volume: 39 Pages: 1588-1604

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mito-mice∆ and mitochondrial DNA mutator mice as models of human osteoporosis caused not by aging but by hyperparathyroidism2018

    • Author(s)
      Mito Takayuki、Tani Haruna、Suzuki Michiko、Ishikawa Kaori、Nakada Kazuto、Hayashi Jun-Ichi
    • Journal Title

      Experimental Animals

      Volume: 67 Issue: 4 Pages: 509-516

    • DOI

      10.1538/expanim.18-0060

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal.2018

    • Author(s)
      Tani H, Ohnishi S, Shitara H, Mito T, Yamaguchi M, Yonekawa H, Hashizume O, Ishikawa K, Nakada K, Hayashi JI.
    • Journal Title

      Sci Rep.

      Volume: 8 Issue: 1 Pages: 425-425

    • DOI

      10.1038/s41598-017-18828-3

    • NAID

      120007134501

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RLR-mediated antiviral innate immunity requires oxidative phosphorylation activity2017

    • Author(s)
      Yoshizumi T, Imamura H, Taku T, Kuroki T, Kawaguchi A, Ishikawa K, Nakada K, Koshiba T
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 5379-5379

    • DOI

      10.1038/s41598-017-05808-w

    • NAID

      120007128833

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel mutation in TAZ causes mitochondrial respiratory chain disorder without cardiomyopathy2016

    • Author(s)
      Borna NN, Kishita Y, Ishikawa K, Nakada K, Hayashi JI, Tokuzawa Y, Kohda M, Nyuzuki H, Yamashita-Sugahara Y, Nasu T, Takeda A, Murayama K, Ohtake A, Okazaki Y
    • Journal Title

      J Hum Genet

      Volume: 62 Issue: 5 Pages: 539-547

    • DOI

      10.1038/jhg.2016.165

    • NAID

      40021210340

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] FGF21 is a biomarker for mitochondrial translation and mtDNA maintenance disorders2016

    • Author(s)
      Lehtonen JM, et al.(Nakada Kは24名中22番目の著者)
    • Journal Title

      Neurology

      Volume: 87 Issue: 22 Pages: 2290-2299

    • DOI

      10.1212/wnl.0000000000003374

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mitochonic Acid 5 Binds Mitochondria and Ameliorates Renal Tubular and Cardiac Myocyte Damage.2016

    • Author(s)
      Suzuki T, Kanno SI, Abe T. et al
    • Journal Title

      J Am Soc Nephrol.

      Volume: 27 Issue: 7 Pages: 1-8

    • DOI

      10.1681/asn.2015060623

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 突然変異型ミトコンドリアゲノムを含有するモデルマウス2016

    • Author(s)
      小笠原絵美、中田和人
    • Journal Title

      医学のあゆみ

      Volume: 260 Pages: 73-79

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 変異型ミトコンドリアゲノムの逆遺伝学:多様な病態形成機構の理解に向けて2019

    • Author(s)
      中田和人
    • Organizer
      第19回日本生殖工学会学術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] モデルマウスから探るミトコンドリアセントラルドグマの破綻病理2018

    • Author(s)
      中田和人
    • Organizer
      第56回日本神経眼科学会総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Reverse genetic studies on mammalian mitochondrial DNA with pathogenic mutations.2018

    • Author(s)
      Kazuto Nakada
    • Organizer
      Asian Society for Mitochondrial and Medicine (ASMRM2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マウス逆遺伝学から理解するミトコンドリアゲノム変異の多階層病理2018

    • Author(s)
      中田和人
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ミトコンドリア病を発症したモデルマウスにおける インドール化合物 Mitochonic acid 5 の 有効性に関する基礎研究2017

    • Author(s)
      小笠原絵美、石原直忠、阿部高明、中田和人
    • Organizer
      日本ミトコンドリア学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミトコンドリア・セントラルドグマの破綻病理2017

    • Author(s)
      中田和人
    • Organizer
      The 7th Basic Science Seminar of Women’s Health Care
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 変異型mtDNAによる多階層病理2016

    • Author(s)
      中田和人
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ミトコンドリアセントラルドグマの破綻病理2016

    • Author(s)
      中田和人
    • Organizer
      第16回日本抗加齢医学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-06-11
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Remarks] 筑波大学生命環境系 中田ー石川研究室

    • URL

      http://www.biol.tsukuba.ac.jp/nakada_ishikawa/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 筑波大学生命環境系 中田・石川研究室

    • URL

      http://www.biol.tsukuba.ac.jp/~jih-kzt/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 筑波大学生命環境系 中田・石川研究室

    • URL

      http://www.biol.tsukuba.ac.jp/~jih-kzt/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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