Development of houndshark nanodoy by combination of wheat cell-free and NGS technologies

• Project/Area Number: 16H04729
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: System genome science
• Research Institution: Ehime University
• Principal Investigator: SAWASAKI TATSUYA 愛媛大学, プロテオサイエンスセンター, 教授 (50314969)
• Research Collaborator: Narita Mitsuyoshi 愛媛県栽培資源研究所
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: cell-free / antibody / shark / nanobody / fukabody / houdshark / NGS / タンパク質 / ナノボディ / 無細胞 / 抗体 / サメ / Fukabody / ドチザメ / バイオテクノロジー / 免疫学 / 生体分子 / 生体機能利用 / 蛋白質 / トランスクリプトーム

Outline of Final Research Achievements

We used GFP (green fluorescent protein) as a model for shark nonobody, which we called shark nanobody as fukabody. After GFP immunization to houndshark, some houndsharks indicated specific immunoresponse to GFP. We screened GFP-binding fukabody by phage display at totally three-time rounds. DNA fragments from each round were analyzed its sequences by next generation sequencing (NGS) technology. Enriched DNA fragments were found, and then their proteins were synthesized by wheat cell-free system. Enriched clones indicated binding ability to GFP antigen. We are successful to develop construction system for fukabody by combination of wheat cell-free and NGS technologies.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、日本産エイラクブカを用いて、抗原特異的なフカボディを単離する技術が確立できた。フカボディは、ナノボディよりさらに抗原タンパク質の溝を認識することが本研究成果により分かってきたので、フカボディを利用した創薬ができる基盤技術となった。また、本研究成果により古代ザメとは異なり、現在に広く存在するサメは、哺乳類に近い免疫応答系を有していることが明らかとなった。

Research Products

• [Journal Article] The ring-type E3 ubiquitin ligase JUL1 targets the VQ-motif protein JAV1 to coordinate jasmonate signaling (2019)
  • Author(s): Mohamed R. M. Ali, Takuya Uemura, Abdelaziz Ramadan, Kyoko Adachi, Keiichirou Nemoto, Akira Nozawa, Ryosuke Hoshino, Hiroshi Abe, Tatsuya Sawasaki, Gen-ichiro Arimura
  • Journal Title: Plant Physiology Volume: 179 Issue: 4 Pages: 1273-1284
  • DOI: 10.1104/pp.18.00715
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PIM kinases facilitate lentiviral evasion from SAMHD1 restriction via Vpx phosphorylation (2019)
  • Author(s): Miyakawa Kei、Matsunaga Satoko、Yokoyama Masaru、Nomaguchi Masako、Kimura Yayoi、Nishi Mayuko、Kimura Hirokazu、Sato Hironori、Hirano Hisashi、Tamura Tomohiko、Akari Hirofumi、Miura Tomoyuki、Adachi Akio、Sawasaki Tatsuya、Yamamoto Naoki、Ryo Akihide
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1844-1844
  • DOI: 10.1038/s41467-019-09867-7
  • NAID: 120006949469
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The malaria parasite RhopH protein complex interacts with erythrocyte calmyrin identified from a comprehensive erythrocyte protein library (2018)
  • Author(s): Toyokazu Miura, Satoru Takeo, Edward H Ntege, Hitoshi Otsuki, Tatsuya Sawasaki, Tomoko Ishino, Eizo Takashima, Takafumi Tsuboi
  • Journal Title: Biochemical and biophysical research communications Volume: 500 Issue: 2 Pages: 261-267
  • DOI: 10.1016/j.bbrc.2018.04.056
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5. (2018)
  • Author(s): Hashimoto Y, et al.
  • Journal Title: Sci Rep. Volume: 8 Issue: 1 Pages: 8383-8383
  • DOI: 10.1038/s41598-018-26560-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The ubiquitin ligase RNF38 promotes RUNX1 ubiquitination and enhances RUNX1-mediated suppression of erythroid transcription program (2018)
  • Author(s): Yonezawa Taishi、Takahashi Hirotaka、Shikata Shiori、Sawasaki Tatsuya、Kitamura Toshio、Goyama Susumu
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 505 Issue: 3 Pages: 905-909
  • DOI: 10.1016/j.bbrc.2018.10.006
  • Peer Reviewed / Open Access
• [Journal Article] Amyloid β directly interacts with NLRP3 to initiate inflammasome activation: identification of an intrinsic NLRP3 ligand in a cell-free system (2018)
  • Author(s): Nakanishi Ayaka、Kaneko Naoe、Takeda Hiroyuki、Sawasaki Tatsuya、Morikawa Shinnosuke、Zhou Wei、Kurata Mie、Yamamoto Toshihiro、Akbar Sheikh Mohammad Fazle、Zako Tamotsu、Masumoto Junya
  • Journal Title: Inflammation and Regeneration Volume: 38 Issue: 1 Pages: 27-27
  • DOI: 10.1186/s41232-018-0085-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] チロシンリン酸化によるジベレリン応答の新しい制御機構 (2018)
  • Author(s): 根本圭一郎、澤崎達也
  • Journal Title: バイオインダストリー Volume: 76 Pages: 232-225
  • NAID: 40021578645
• [Journal Article] Identification of new abscisic acid receptor agonists using a wheat cell-free based drug screening system (2018)
  • Author(s): Nemoto Keiichirou、Kagawa Makiko、Nozawa Akira、Hasegawa Yoshinori、Hayashi Minoru、Imai Kenichiro、Tomii Kentaro、Sawasaki Tatsuya
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 4268-4268
  • DOI: 10.1038/s41598-018-22538-9
  • Peer Reviewed / Open Access
• [Journal Article] Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes (2018)
  • Author(s): Suzuki Yasuyuki、Ogasawara Tomio、Tanaka Yuki、Takeda Hiroyuki、Sawasaki Tatsuya、Mogi Masaki、Liu Shuang、Maeyama Kazutaka
  • Journal Title: Frontiers in Pharmacology Volume: 9 Pages: 38-38
  • DOI: 10.3389/fphar.2018.00038
  • Peer Reviewed / Open Access
• [Journal Article] DANFIN functions as an inhibitor of transcription factor NF-κB and potentiates the antitumor effect of bortezomib in multiple myeloma (2018)
  • Author(s): Uematsu Atsushi、Kido Kohki、Manabe Erika、Takeda Hiroyuki、Takahashi Hirotaka、Hayashi Minoru、Imai Yuuki、Sawasaki Tatsuya
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 3 Pages: 2289-2295
  • DOI: 10.1016/j.bbrc.2017.12.142
  • Peer Reviewed / Open Access
• [Journal Article] Tyrosine phosphorylation of the GARU E3 ubiquitin ligase promotes gibberellin signalling by preventing GID1 degradation (2017)
  • Author(s): Nemoto K., Ramadan A., Arimura G., Imai K., Tomii K., Shinozaki K., Sawasaki T.
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1004-1004
  • DOI: 10.1038/s41467-017-01005-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Applications of reconstituted inflammasomes in a cell-free system to drug discovery and elucidation of the pathogenesis of autoinflammatory diseases. (2017)
  • Author(s): Kaneko N, Iwasaki T, Ito Y, Takeda H, Sawasaki T, Morikawa S, Nakano N, Kurata M, Masumoto J.
  • Journal Title: Inflammation and Regeneration Volume: 印刷中 Issue: 1 Pages: 9-9
  • DOI: 10.1186/s41232-017-0040-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression (2017)
  • Author(s): Sun Suofeng、Nakashima Kenji、Ito Masahiko、Li Yuan、Chida Takeshi、Takahashi Hirotaka、Watashi Koichi、Sawasaki Tatsuya、Wakita Takaji、Suzuki Tetsuro
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12874-12874
  • DOI: 10.1038/s41598-017-12497-y
  • Peer Reviewed / Open Access
• [Journal Article] Claudin-5-Binders Enhance Permeation of Solutes across the Blood-Brain Barrier in a Mammalian Model (2017)
  • Author(s): Hashimoto Yosuke、Shirakura Keisuke、Okada Yoshiaki、Takeda Hiroyuki、Endo Kohki、Tamura Maki、Watari Akihiro、Sadamura Yoshifusa、Sawasaki Tatsuya、Doi Takefumi、Yagi Kiyohito、Kondoh Masuo
  • Journal Title: J Pharmacol Exp Ther. Volume: 363 Issue: 2 Pages: 275-283
  • DOI: 10.1124/jpet.117.243014
  • Peer Reviewed
• [Journal Article] Efficiency and Safety of CRAC Inhibitors in Human Rheumatoid Arthritis Xenograft Models (2017)
  • Author(s): Liu Shuang、Hasegawa Hitoshi、Takemasa Erika、Suzuki Yasuyuki、Oka Keizou、Kiyoi Takeshi、Takeda Hiroyuki、Ogasawara Tomio、Sawasaki Tatsuya、Yasukawa Masaki、Maeyama Kazutaka
  • Journal Title: The Journal of Immunology Volume: 199 Issue: 5 Pages: 1584-1595
  • DOI: 10.4049/jimmunol.1700192
  • Peer Reviewed / Open Access
• [Journal Article] Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems (2017)
  • Author(s): Nemoto Keiichirou、Nozawa Akira、Yamanaka Satoshi、Nomura Shunsuke、Kido Kohki、Sawasaki Tatsuya
  • Journal Title: Plant Receptor Kinases Volume: 1621 Pages: 113-120
  • DOI: 10.1007/978-1-4939-7063-6_11
  • ISBN: 9781493970629, 9781493970636
• [Journal Article] Cell-Free Synthesis of Plant Receptor Kinases (2017)
  • Author(s): Nozawa Akira、Nemoto Keiichirou、Nomura Shunsuke、Yamanaka Satoshi、Kido Kohki、Sawasaki Tatsuya
  • Journal Title: Plant Receptor Kinases Volume: 1621 Pages: 37-46
  • DOI: 10.1007/978-1-4939-7063-6_4
  • ISBN: 9781493970629, 9781493970636
• [Journal Article] CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag (2017)
  • Author(s): Takeda Hiroyuki、Zhou Wei、Kido Kohki、Suno Ryoji、Iwasaki Takahiro、Kobayashi Takuya、Sawasaki Tatsuya
  • Journal Title: PLOS ONE Volume: 12 Issue: 5 Pages: e0178246-e0178246
  • DOI: 10.1371/journal.pone.0178246
  • Peer Reviewed / Open Access
• [Journal Article] The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1?RUNX1T1 (2017)
  • Author(s): Yonezawa Taishi、Takahashi Hirotaka、Shikata Shiori、Liu Xiaoxiao、Tamura Moe、Asada Shuhei、Fukushima Tsuyoshi、Fukuyama Tomofusa、Tanaka Yosuke、Sawasaki Tatsuya、Kitamura Toshio、Goyama Susumu
  • Journal Title: Journal of Biological Chemistry Volume: 292 Issue: 30 Pages: 12528-12541
  • DOI: 10.1074/jbc.m117.785675
  • Peer Reviewed
• [Journal Article] Angubindin-1, a novel paracellular absorption enhancer acting at the tricellular tight junction. (2017)
  • Author(s): Krug SM, Hayaishi T, Iguchi D, Watari A,Takahashi A, Fromm M, Nagahama M, Takeda H, Okada Y,Sawasaki T, Doi T, Yagi K, Kondoh M
  • Journal Title: J. Control. Release. Volume: 260 Pages: 1-11
  • DOI: 10.1016/j.jconrel.2017.05.024
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] AGIA タグシステム:細胞生物学研究に最適な高感度検出および キャプチャー用ペプチドタグ (2017)
  • Author(s): 竹田浩之、澤崎達也
  • Journal Title: 生化学 Volume: 89 Pages: 302-307
  • NAID: 40021203473
• [Journal Article] Interaction between RNF8 and DYRK2 is required for the recruitment of DNA repair molecules to DNA double-strand breaks. (2017)
  • Author(s): Yamamoto T, Taira Nihira N, Yogosawa S, Aoki K, Takeda H, Sawasaki T, Yoshida K. Interaction between RNF8 and DYRK2 is required for the recruitment of DNA repair molecules to DNA double-strand breaks.
  • Journal Title: FEBS Letters Volume: 591 Issue: 6 Pages: 842-853
  • DOI: 10.1002/1873-3468.12596
  • Peer Reviewed
• [Journal Article] HTLV-1 Tax induces formation of the active macromolecular IKK complex by generating Lys63- and Met1-linked hybrid polyubiquitin chains. (2017)
  • Author(s): Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, Tanaka K, Takahashi H, Sawasaki T, Inoue S, Oshiumi H, Seya T, Nakano H, Tanaka Y, Iwai K, and Inoue J.
  • Journal Title: PLoS Pathog. Volume: 13 Issue: 12 Pages: e1006162-e1006162
  • DOI: 10.1371/journal.ppat.1006162
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability. (2017)
  • Author(s): Ogawa S, Miyamoto K, Nemoto K, Sawasaki T, Yamane H, Nojiri H, Okada K.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 40175-40175
  • DOI: 10.1038/srep40175
  • Peer Reviewed / Open Access
• [Journal Article] Plant Aurora kinases interact with and phosphorylate transcription factors. (2016)
  • Author(s): Takagi, M., Sakamoto, T.,Suzuki, R.,Nemoto, K., Obayashi, T., Matsunaga, T. M., Hirakawa, T., Kurihara, D., Nariai, Y., Urano, T., Sawasaki, T., Matsunaga, S.
  • Journal Title: JOURNAL OF PLANT RESEARCH Volume: 129 Issue: 6 Pages: 1165-1178
  • DOI: 10.1007/s10265-016-0860-x
  • NAID: 40020988231
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. (2016)
  • Author(s): Kuwahara M, Ise W, Ochi M, Suzuki J, Kometani K, Maruyama S, Izumoto M, Matsumoto A, Takemori N, Takemori A, Shinoda K, Nakayama T, Ohara O, Yasukawa M, Sawasaki T, Kurosaki T, Yamashita M.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 12596-12596
  • DOI: 10.1038/ncomms12596
  • Peer Reviewed / Open Access
• [Journal Article] Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. (2016)
  • Author(s): Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, and Tokunaga F.
  • Journal Title: Nat. Commun. Volume: 7 Issue: 1 Pages: 12547-12547
  • DOI: 10.1038/ncomms12547
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage (2016)
  • Author(s): Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N
  • Journal Title: Biochim Biophys Acta Volume: 1863 Issue: 11 Pages: 2766-2783
  • DOI: 10.1016/j.bbamcr.2016.08.010
  • Peer Reviewed
• [Journal Article] MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis. (2016)
  • Author(s): Santolini M, Sakakibara I, Gauthier M, Ribas-Aulinas F, Takahashi H, Sawasaki T, Mouly V, Concordet JP, Defossez PA, Hakim V, Maire P.
  • Journal Title: Nucleic Acids Research Volume: 44 Issue: 18 Pages: 8621-8640
  • DOI: 10.1093/nar/gkw512
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset Sarcoidosis. (2016)
  • Author(s): Iwasaki T, Kaneko N, Ito Y, Takeda H, Sawasaki T, Heike T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J.
  • Journal Title: The Scientific World Journal Volume: － Pages: 2597376-2597376
  • DOI: 10.1155/2016/2597376
  • NAID: 120005819501
  • Peer Reviewed / Open Access
• [Journal Article] H11/HSPB8 Restricts HIV-2 Vpx to Restore the Anti-Viral Activity of SAMHD1. (2016)
  • Author(s): Kudoh A, Miyakawa K, Matsunaga S, Matsushima Y, Kosugi I, Kimura H, Hayakawa S, Sawasaki T, Ryo A.
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 883-883
  • DOI: 10.3389/fmicb.2016.00883
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis. (2016)
  • Author(s): Yano T, Takeda H, Uematsu A, Yamanaka S, Nomura S, Nemoto K, Iwasaki T, Takahashi H, Sawasaki T.
  • Journal Title: PLoS One Volume: 11 Issue: 6 Pages: e0156716-e0156716
  • DOI: 10.1371/journal.pone.0156716
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins. (2016)
  • Author(s): Takahashi H, Uematsu A, Yamanaka S, Imamura M, Nakajima T, Doi K, Yasuoka S, Takahashi C, Takeda H, Sawasaki T.
  • Journal Title: PLoS One Volume: 11 Issue: 6 Pages: e0156718-e0156718
  • DOI: 10.1371/journal.pone.0156718
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gfi1, a transcriptional repressor, inhibits the induction of the T helper type 1 programme in activated CD4 T cells (2016)
  • Author(s): Junpei Suzuki, Saho Maruyama, Hidekazu Tamauchi, Makoto Kuwahara, Mika Horiuchi, Masumi Mizuki, Mizuki Ochi, Tatsuya Sawasaki, Jinfang Zhu, Masaki Yasukawa, Masakatsu Yamashita
  • Journal Title: Immunology Volume: 147 Issue: 4 Pages: 476-487
  • DOI: 10.1111/imm.12580
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] くすりはタンパク質を狙う (2018)
  • Author(s): 澤崎達也
  • Organizer: 第91回日本薬理学会年会／第 18 回国際薬理学・臨床薬理学会議（WCP2018）｜市民公開講座
  • Invited
• [Presentation] コムギ無細胞系を基盤とした薬剤開発技術 (2018)
  • Author(s): 澤崎達也
  • Organizer: 第18回 日本蛋白質科学会
  • Invited
• [Presentation] コムギ胚芽無細胞系を用いたヒトデcaspase-3/9とsfApaf-1の合成 (2018)
  • Author(s): 高岡幸恵、竹田浩之、岩崎隆宏、澤崎達也、千葉和義
  • Organizer: 第41回分子生物学会年会
• [Presentation] Acceleration of RNA biology-targeting drug discovery in cancer using cell-free technology (2018)
  • Author(s): 竹田浩之、澤崎達也
  • Organizer: 第77回日本癌学会学術総会
  • Invited
• [Presentation] 愛媛県産ドチザメ近縁種を用いたvNAR抗体作製 (2017)
  • Author(s): 善家 弘貴 , 柴田 祐稀, 清水 孝昭, 成田 公義, 岸本 聡, 中村 翠珠, 坂口 秀雄, 伊東 祐 二, 澤崎 達也, 竹田 浩之
  • Organizer: ConBio2017
• [Presentation] コムギ無細胞系を活用した 植物チロシンキナーゼTAGKの発見と新しいジベレリン応答制御機構の同定 (2017)
  • Author(s): 澤崎達也
  • Organizer: 第22回 東京大学生物生産工学研究センターシンポジウム
  • Invited
• [Presentation] コムギ無細胞系を基盤とした新規タグ-抗体システムと薬剤探索技術の開発 (2017)
  • Author(s): 澤崎達也
  • Organizer: 岩手生物工学研究センター第２３７回公開セミナー
  • Invited
• [Presentation] 大学・自治体協同による地方大学発バイオベンチャーの生き残り術 (2016)
  • Author(s): 澤崎達也
  • Organizer: 平成２８年度 地域コトづくりセンター報告会＆シンポジウム
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2016-11-25
  • Invited
• [Presentation] プロテオーム大規模解析が切り開く新たな生化学研究：コムギ無細胞系を基盤とした２つの大規模生化学的解析法：ヒト２万種プロテインアレイによるインタラクトーム解析とケミカルバイオロジーに向けた薬剤開発 (2016)
  • Author(s): 澤崎達也, 高橋宏隆
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 東北大学・仙台国際センター（宮城研仙台市）
  • Year and Date: 2016-09-25
  • Invited
• [Presentation] コムギ無細胞系を基盤とした蛋白質の合成・機能解析技術 (2016)
  • Author(s): 澤崎達也
  • Organizer: 第40回蛋白質と酵素の構造と機能に関する九州シンポジウム
  • Place of Presentation: 指宿ベイテラス（鹿児島県指宿市）
  • Year and Date: 2016-08-26
  • Invited
• [Presentation] コムギ無細胞技術を基盤とした研究支援 (2016)
  • Author(s): 澤崎達也
  • Organizer: 神戸大学バイオシグナル総合研究センター設置記念シンポジウム
  • Place of Presentation: 神戸大学（兵庫県神戸市）
  • Year and Date: 2016-08-22
  • Invited
• [Presentation] 医学・農学ケミカルバイオロジーに向けたコムギ無細胞基盤薬剤開発 (2016)
  • Author(s): 澤崎達也
  • Organizer: アグリバイオ工学研究部門公開セミナー
  • Place of Presentation: 東京理科大学（東京都葛飾区）
  • Year and Date: 2016-06-20
  • Invited
• [Remarks] 澤崎研のホームページ
  • URL: http://www.pros.ehime-u.ac.jp/cell-free/index.html
• [Remarks] 澤崎研HP
  • URL: http://www.pros.ehime-u.ac.jp/cell-free/index.html