| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
|---|
| Outline of Final Research Achievements |
We used GFP (green fluorescent protein) as a model for shark nonobody, which we called shark nanobody as fukabody. After GFP immunization to houndshark, some houndsharks indicated specific immunoresponse to GFP. We screened GFP-binding fukabody by phage display at totally three-time rounds. DNA fragments from each round were analyzed its sequences by next generation sequencing (NGS) technology. Enriched DNA fragments were found, and then their proteins were synthesized by wheat cell-free system. Enriched clones indicated binding ability to GFP antigen. We are successful to develop construction system for fukabody by combination of wheat cell-free and NGS technologies.
|
