Development of houndshark nanodoy by combination of wheat cell-free and NGS technologies
Project/Area Number |
16H04729
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
System genome science
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Research Institution | Ehime University |
Principal Investigator |
SAWASAKI TATSUYA 愛媛大学, プロテオサイエンスセンター, 教授 (50314969)
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Research Collaborator |
Narita Mitsuyoshi 愛媛県栽培資源研究所
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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Keywords | cell-free / antibody / shark / nanobody / fukabody / houdshark / NGS / タンパク質 / ナノボディ / 無細胞 / 抗体 / サメ / Fukabody / ドチザメ / バイオテクノロジー / 免疫学 / 生体分子 / 生体機能利用 / 蛋白質 / トランスクリプトーム |
Outline of Final Research Achievements |
We used GFP (green fluorescent protein) as a model for shark nonobody, which we called shark nanobody as fukabody. After GFP immunization to houndshark, some houndsharks indicated specific immunoresponse to GFP. We screened GFP-binding fukabody by phage display at totally three-time rounds. DNA fragments from each round were analyzed its sequences by next generation sequencing (NGS) technology. Enriched DNA fragments were found, and then their proteins were synthesized by wheat cell-free system. Enriched clones indicated binding ability to GFP antigen. We are successful to develop construction system for fukabody by combination of wheat cell-free and NGS technologies.
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果により、日本産エイラクブカを用いて、抗原特異的なフカボディを単離する技術が確立できた。フカボディは、ナノボディよりさらに抗原タンパク質の溝を認識することが本研究成果により分かってきたので、フカボディを利用した創薬ができる基盤技術となった。また、本研究成果により古代ザメとは異なり、現在に広く存在するサメは、哺乳類に近い免疫応答系を有していることが明らかとなった。
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains.2017
Author(s)
Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, Tanaka K, Takahashi H, Sawasaki T, Inoue S, Oshiumi H, Seya T, Nakano H, Tanaka Y, Iwai K, Inoue JI.
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Journal Title
PLOS Pathogens
Volume: 13
Pages: e1006162
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Plant Aurora kinases interact with and phosphorylate transcription factors.2016
Author(s)
Takagi M, Sakamoto T, Suzuki R, Nemoto K, Obayashi T, Hirakawa T, Matsunaga TM, Kurihara D, Nariai Y, Urano T, Sawasaki T, Matsunaga S.
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Journal Title
Journal of Plant Research
Volume: 129
Pages: 1165-1178.
DOI
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Peer Reviewed
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[Journal Article] Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop.2016
Author(s)
Kuwahara M, Ise W, Ochi M, Suzuki J, Kometani K, Maruyama S, Izumoto M, Matsumoto A, Takemori N, Takemori A, Shinoda K, Nakayama T, Ohara O, Yasukawa M, Sawasaki T, Kurosaki T, Yamashita M.
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Journal Title
Nature Communications
Volume: 7
Pages: 12596
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis.2016
Author(s)
Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F.
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Journal Title
Nature Communications
Volume: 7
Pages: 12547
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage.2016
Author(s)
Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N.
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Journal Title
Biochimica et Biophysica Acta
Volume: 1863
Pages: 2766-2783
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Peer Reviewed
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[Journal Article] MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis.2016
Author(s)
Santolini M, Sakakibara I, Gauthier M, Ribas-Aulinas F, Takahashi H, Sawasaki T, Mouly V, Concordet JP, Defossez PA, Hakim V, Maire P.
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Journal Title
Nucleic Acids Research
Volume: 44
Pages: 8621-8640
DOI
Related Report
Peer Reviewed
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[Journal Article] Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset Sarcoidosis.2016
Author(s)
Iwasaki T, Kaneko N, Ito Y, Takeda H, Sawasaki T, Heike T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J.
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Journal Title
The Scientific World Journal
Volume: -
Pages: 2597376
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Peer Reviewed
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[Journal Article] Gfi1, a transcriptional repressor, inhibits the induction of the T helper type 1 programme in activated CD4 T cells.2016
Author(s)
Suzuki J, Maruyama S, Tamauchi H, Kuwahara M, Horiuchi M, Mizuki M, Ochi M, Sawasaki T, Zhu J, Yasukawa M, Yamashita M.
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Journal Title
Immunology
Volume: 147
Pages: 476-487
DOI
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Peer Reviewed
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