| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Metabolon is a multi-enzyme complex assembled with metabolic enzymes that constitute a biosynthetic pathway. Clustering metabolic enzymes increases the in vivo local concentration of metabolic enzymes and accelerates flux of the substrates and products between metabolic enzymes, which is thought to enhance biosynthesis even under the condition of low expression levels of metabolic enzymes. In this study, we addressed to the improvement of the efficiency of biosynthesis by the assembly of synthetic metabolon, which is artificially assembled with utilizing interactions between the scaffolds and enzymes of plant biomass-degrading multi-enzyme complex. The biosynthetic pathways of polyketides were integrated into the Escherichia coli genome using a site-specific recombinase. Although the expression levels of metabolic enzymes decreased by the genomic integration of biosynthetic pathways, the results indicated that clustering metabolic enzymes can improve the efficiency of biosynthesis.
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