| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
This research aimed to study the mechanism to load PCNA on leading- and lagging-strands coordinately during replication in eukaryotes. I have studied the interaction between the second PCNA loader Ctf18-RFC and the leading strand DNA polymerase Pol ε. I elucidated that Ctf18-RFC actively loads PCNA only when it complexes with DNA polymerase ε. Furthermore, these two complexes together exhibit a stable DNA synthesis through a cycle of [DNA synthesis by Pol ε－PCNA] -> [Stalling of the DNA synthesis] -> [PCNA loading by Ctf18-RFC] -> [Restart of DNA synthesis by Pol ε－PCNA－Ctf18-RFC]. This result indicated that Ctf18-RFC is involved in the leading-strand DNA polymerase complex and actively loads PCNA on the leading strands. Considering the role of RFC for loading of PCNA on the lagging strands, RFC and Ctf18-RFC will coordinate PCNA loading on both strands and play a role to distinguish DNA polymerases for syntheses of the two DNA strands.
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