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The active chromatin domain that is regulated by the non-coding RNAs in recurrent breast cancer

Research Project

Project/Area Number 16H04744
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionJapanese Foundation for Cancer Research (2017-2018)
Kumamoto University (2016)

Principal Investigator

Saitoh Noriko  公益財団法人がん研究会, がん研究所 がん生物部, 部長 (40398235)

Research Collaborator YAMAMOTO tatsuro  
SAKAMOTO chiyomi  
TACHIWANA hiroaki  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords非コードRNA / クロマチン / 細胞核 / 乳がん / クロマチンドメイン / 核内非コードRNA / ESR1 / RNA / 核内構造 / レスベラトロール
Outline of Final Research Achievements

Approximately 70% of breast cancers is estrogen receptor (ER)-positive, which highly express the ER protein. The endocrine therapy that inhibits estrogenic action is effective for this type of breast cancer, however, the cancer acquires the therapy resistance after long term of the treatment, resulting in recurrence. In this study, using the endocrine-therapy resistant breast cancer cell model, I have revealed a molecular mechanism underlying the overexpression of ESR1, the gene for ER during the acquisition of the therapy resistance. I found that the non-coding RNAs, Eleanors regulate the mega-base chromatin domain and expression of the multiple genes involving breast cancer including ESR1.

Academic Significance and Societal Importance of the Research Achievements

遺伝子の発現制御はほぼ全ての生命現象に重要で、その異常はがんを含む疾患に深く関わる。本研究成果では、乳がんに重要なESR1遺伝子の発現が非コードRNAエレノアによるクロマチンドメインの制御を介して行われるという、学術的に新しい知見を得られた。また、非コードRNAを標的とする再発乳がんの診断や治療への道筋をつける成果を得た。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (51 results)

All 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (30 results) (of which Int'l Joint Research: 9 results,  Invited: 23 results) Book (4 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Univ of Massachusetts Med Sch/Mindshare Medical Inc(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Harbin Medical University(中国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] NIA, NIH(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Harbin Medical University(中国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms2018

    • Author(s)
      Takagi Masatoshi、Ono Takao、Natsume Toyoaki、Sakamoto Chiyomi、Nakao Mitsuyoshi、Saitoh Noriko、Kanemaki Masato T.、Hirano Tatsuya、Imamoto Naoko
    • Journal Title

      Journal of Cell Science

      Volume: 131 Pages: 212092-212092

    • DOI

      10.1242/jcs.212092

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An asymmetric centromeric nucleosome2018

    • Author(s)
      Ichikawa Yuichi、Saitoh Noriko、Kaufman Paul D
    • Journal Title

      eLife

      Volume: 7

    • DOI

      10.7554/elife.37911

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA2018

    • Author(s)
      Yamamoto Tatsuro、Sakamoto Chiyomi、Tachiwana Hiroaki、Kumabe Mitsuru、Matsui Toshiro、Yamashita Tadatoshi、Shinagawa Masatoshi、Ochiai Koji、Saitoh Noriko、Nakao Mitsuyoshi
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 15202-15202

    • DOI

      10.1038/s41598-018-33227-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The SETD8/PR-Set7 Methyltransferase Functions as a Barrier to Prevent Senescence-Associated Metabolic Remodeling.2017

    • Author(s)
      Tanaka, H., Takebayashi S. I., Sakamoto, A., Igata, T., Nakatsu, Y., Saitoh, N., Hino, S., and Nakao, M.
    • Journal Title

      Cell Rep.

      Volume: 18 Issue: 9 Pages: 2148-2161

    • DOI

      10.1016/j.celrep.2017.02.021

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of estrogen receptor beta gene methylation in autistic males in a Chinese Han population2017

    • Author(s)
      Wang, X., Liang, S., Sun, Y., Li, H., Endo, F., Nakao, M., Saitoh, N.* and Wu, L.*
    • Journal Title

      Metab Brain Dis

      Volume: - Issue: 4 Pages: 1033-1042

    • DOI

      10.1007/s11011-017-9990-7

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ2017

    • Author(s)
      Ono Takao、Sakamoto Chiyomi、Nakao Mitsuyoshi、Saitoh Noriko、Hirano Tatsuya
    • Journal Title

      Molecular Biology of the Cell

      Volume: 28 Issue: 21 Pages: 2875-2886

    • DOI

      10.1091/mbc.e17-04-0252

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The 4D nucleome in Krak?w - Prospects for an emerging field2017

    • Author(s)
      Dobrucki Jurek、Saitoh Noriko
    • Journal Title

      Nucleus

      Volume: 8 Issue: 5 Pages: 447-448

    • DOI

      10.1080/19491034.2017.1347243

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Roles of long non-coding RNAs in chromosome domains2016

    • Author(s)
      Tomita, S., Abdalla, M. O. A., Fujiwara, S., Yamamoto, T., Iwase, H., Nakao, M. and Saitoh, N.*.
    • Journal Title

      WIREs RNA

      Volume: - Issue: 2 Pages: 30366-30374

    • DOI

      10.1002/wrna.1384

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nuclear extrusion precedes discharge of genomic DNA fibers during tunicamycin-induced neutrophil extracellular trap-osis (NETosis)-like cell death in cultured human leukemia cells2016

    • Author(s)
      Nakayama, T., Saitoh, N, Morotomi-Yano, K., Yano, K.I., Nakao, M, Saitoh, H
    • Journal Title

      Cell Biol Int

      Volume: 印刷中 Issue: 5 Pages: 597-602

    • DOI

      10.1002/cbin.10594

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of the integral nuclear envelope protein SUN1 induces alteration of nucleoli2016

    • Author(s)
      Matsumoto A, Sakamoto C, Matsumori H, Katahira J, Yasuda Y, Yoshidome K, Tsujimoto M, Goldberg IG, Matsuura N, Nakao M, Saitoh S, Hieda M
    • Journal Title

      Nucleus

      Volume: 7(1) Issue: 1 Pages: 68-83

    • DOI

      10.1080/19491034.2016.1149664

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Nuclear non-coding RNAs Eleanors, define the active ESR1 chromatin domain in breast cancer cells.2018

    • Author(s)
      Saitoh, N.
    • Organizer
      Cold Spring Harbor Conferences Asia, RNA Biology.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Saitoh, N. Chromatin regulation by nuclear non-coding RNA in breast cancer cells.2018

    • Author(s)
      Saitoh, N.
    • Organizer
      HiHA 国際ワークショップ ?染色体動態、分配と機能の理解に向かって
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 乳がんの高次クロマチンドメインに関わるノンコーディングRNA.2018

    • Author(s)
      斉藤典子
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 乳がんに関わるノンコーディングRNAと細胞核内ゲノム構造.2018

    • Author(s)
      斉藤 典子
    • Organizer
      千葉大学 第16回クロマチン代謝制御セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 再発乳がんの活性染色体ドメインに関わるエレノアノンコーディングRNA2018

    • Author(s)
      斉藤典子
    • Organizer
      第2回富山RNAワークショップ
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 乳がんと細胞核のかたち2018

    • Author(s)
      斉藤典子
    • Organizer
      新学術領域「動的クロマチン構造と機能」一般公開シンポジウム 遺伝子研究の最前線
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] A cluster of nuclear long non-coding RNAs defines an active chromatin domain in recurrent breast cancer cells2017

    • Author(s)
      Saitoh, N.
    • Organizer
      Wellcome Trust Centre for Cell Biology, Institute of Cell Biology Seminar
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Non-coding RNAs involved in active chromatin domain in breast cancer.2017

    • Author(s)
      Yamamoto, T., Abdalla, M.O., Fujiwara, S., Tomita, S., Nakao, M., and Saitoh, N.
    • Organizer
      HMGU-Japan Epigenetics and Chromatin Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Eleanor non-coding RNA defines the active ESR1 chromatin domain in breast cancer recurrence.2017

    • Author(s)
      Saitoh, N.
    • Organizer
      43rd Naito Conference on “Noncoding RNA: Biology, Disease, & Chemistry”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Active topologically associating domain is formed with non-coding RNA Eleanors in the breast cancer nucleus2017

    • Author(s)
      Yamamoto, T., Abdalla, MO., Nakao, M., and Saitoh, N.
    • Organizer
      Second international meeting on SMC proteins
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A cluster of non-coding RNAs, Eleanors determine the active chromatin domain in breast cancer.2017

    • Author(s)
      Yamamoto, T., Abdalla, MO. Fujiwara, S., Tomita, S., Nakao,M., and Saitoh, N
    • Organizer
      4D Nucleome: The Cell Nucleus in Space and Time
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 活性染色体ドメインに関する核内長鎖ノンコーディングRNA.2017

    • Author(s)
      斉藤典子、山本達郎、モハメドオサマアブダラ、藤原沙織、冨田さおり、前原一満、大川恭行、立和名博昭、中尾光善.
    • Organizer
      生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Non-coding RNAs involved in active chromatin domain formation in breast cancer2017

    • Author(s)
      Saitoh, N.
    • Organizer
      Seminar at Division of Biological Science
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Eleanor non-coding RNAs form the active chromatin domain in breast cancer recurrence.2017

    • Author(s)
      Saitoh,N., Yamamoto, T., Fujiwara, S., Tomita,S. and Nakao, M.
    • Organizer
      The 76th Annual Meeting of the Japanese Cancer Association
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] エピジェネティクスに関わるノンコーディングRNA Eleanor2017

    • Author(s)
      斉藤 典子
    • Organizer
      第5回X染色体研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 乳がんエピジェネティクスに関わる核内長鎖ノンコーディングRNA2017

    • Author(s)
      斉藤 典子
    • Organizer
      千葉県がんセンター集談会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Formation of the membrane-less nuclear organelle, the nucleolus.2017

    • Author(s)
      Saitoh, N., Matsumori, H., Haraguchi, T., Ito. Y., Tokunaga, M., Sogawa, K., Nakao, M.
    • Organizer
      The 40th Annual Meeting of the Japan neuroscience Society
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 核小体の構造維持と物性に関わる60Sリボソームタンパク質2017

    • Author(s)
      斉藤典子
    • Organizer
      日本大学文理学部生命科学科セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 乳がん細胞でノンコーディングRNA群により規定される活性染色体ドメイン2017

    • Author(s)
      斉藤典子、山本達郎、モハメドオサマアブダラ、藤原沙織、冨田さおり、前原一満、大川恭行、中尾光善.
    • Organizer
      第11回日本エピジェネティクス研究会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Nuclear domains implicated in gene regulations and diseases: the nucleolus and RNA clouds.2017

    • Author(s)
      Noriko Saitoh
    • Organizer
      第735回 生医研セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 乳がんにおいて非コードRNA群が規定する活性染色体ドメイン2016

    • Author(s)
      斉藤典子、モハメドオサマアブダラ、藤原沙織、山本達郎、冨田さおり、前原一満、大川恭行、中尾光善.
    • Organizer
      第39回日本分子生物学会年会 シンポジウム1AS13「発生・老化・疾患をつかさどるクロマチンイベント」
    • Place of Presentation
      神奈川県 横浜市 パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ER陽性乳がんにおけるエレノアドメインのダイナミックなクロマチン制御2016

    • Author(s)
      山本達郎、前原一満、大川恭行、三浦尚、平谷伊智朗、斉藤典子、中尾光善
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      神奈川県 横浜市 パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 機械学習画像解析ウインチャームによる胃がん組織の判別2016

    • Author(s)
      安田洋子、徳永和明、坂本智代美、斉藤典子、中尾光善
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      神奈川県 横浜市 パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 非コードRNAにより規定されるクロマチンドメイン2016

    • Author(s)
      斉藤典子、モハメドオサマアブダラ、藤原沙織、山本達郎、冨田さおり、前原一満、大川恭行、中尾光善.
    • Organizer
      遺伝研研究会「クロマチン・細胞核の動的構造変換とエピジェネティック制御」
    • Place of Presentation
      静岡県 三島市 遺伝学研究所
    • Year and Date
      2016-10-27
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] BRD4はMMP2のエピジェネティックな調節を介して口腔扁平上皮癌の転移に関与する2016

    • Author(s)
      山本達郎、廣末晃之、中元雅史、吉田遼司、川原健太、永田将士、田中拓也、斉藤典子、中尾光善、中山秀樹
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      神奈川県 横浜市 パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 乳がんの治療耐性獲得に関わる核内非コードRNAエレノア2016

    • Author(s)
      斉藤典子、モハメドオサマアブダラ、藤原沙織、山本達郎、冨田さおり、前原一満、大川恭行、中尾光善
    • Organizer
      第89回日本生化学会大会 シンポジウム1S15「疾患における細胞核・クロマチンの動態変動 」
    • Place of Presentation
      宮城県 仙台市 東北大学川内北キャンパス
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] siRNA ハイコンテントスクリーニングによる細胞核構築の解析2016

    • Author(s)
      斉藤典子、松森はるか、安田洋子、坂本智代美、檜枝美紀、Ilya G Goldberg、中尾光善
    • Organizer
      バイオイメージ・インフォーマティクス ワークショップ2016
    • Place of Presentation
      大阪府 吹田 大阪大学 吹田キャンパス 銀杏会館
    • Year and Date
      2016-06-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] パターン認識プログラムと細胞核形態に着目した癌組織の定量化2016

    • Author(s)
      安田洋子、徳永和明、坂本智代美、斉藤典子、中尾光善
    • Organizer
      バイオイメージ・インフォマティクスワークショップ2016
    • Place of Presentation
      大阪府 吹田市 大阪大学吹田キャンパス
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] Nuclear structures for gene regulation: the nucleolus and RNA clouds2016

    • Author(s)
      Saitoh, N.
    • Organizer
      NIA/NIH seminar
    • Place of Presentation
      NIA/NIH, Bethesda, Maryland, USA
    • Year and Date
      2016-05-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Machine learning image analysis reveals structural roles of ribosomal proteins in the nucleolus and Diamond-Blackfan anemia2016

    • Author(s)
      Matsumori, H., Osakada, H., Haraguchi, T., Ito, E., Goldberg, I.G., Nakao, M., and Saitoh, N.
    • Organizer
      Cold Spring Harbor Laboratory Meeting; Nuclear organization & function
    • Place of Presentation
      Cold Spring Harbor Laboratory, NY, USA
    • Year and Date
      2016-05-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] 「実験医学」増刊 第36巻第17号、平野達也・胡桃坂仁志 編 「染色体の新常識」2018

    • Author(s)
      野澤竜介、斉藤典子
    • Publisher
      羊土社
    • Related Report
      2018 Annual Research Report
  • [Book] 基礎分子生物学II : 遺伝子発現制御機構 田村隆明・浦聖惠編 編2017

    • Author(s)
      山本達郎、中尾光善、斉藤典子
    • Publisher
      東京化学同人
    • Related Report
      2017 Annual Research Report
  • [Book] 核内イベントの時空間制御2017

    • Author(s)
      山本達郎、中尾光善、斉藤典子.
    • Publisher
      医学書院
    • Related Report
      2017 Annual Research Report
  • [Book] 基礎分子生物学II : 遺伝子発現制御機構 (田村隆明・浦聖惠 編) クロマチンから核構造へ2017

    • Author(s)
      山本達郎、中尾光善、斉藤典子
    • Publisher
      東京化学同人
    • Related Report
      2016 Annual Research Report
  • [Remarks] がん研究所

    • URL

      https://www.jfcr.or.jp/laboratory/department/cancer_biology/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] 熊本大学発生医学研究所

    • URL

      http://www.imeg.kumamoto-u.ac.jp/

    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] グリセオリンIの作用機序とその利用2018

    • Inventor(s)
      山本達郎、立和名博昭、斉藤典子、井出剛、落合孝次
    • Industrial Property Rights Holder
      公益財団法人がん研究会、大豆エナジー株式会社
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-192177
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2022-01-28  

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