| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Outline of Final Research Achievements |
In order to elucidate the details of the destabilization of the enzyme-substrate complex, we established a base for high-resolution X-ray crystallographic analysis while avoiding the influence of crystal damage by X-rays. First, we enabled large-scale sample preparation comparing to several times larger than conventional ones. We then identified the crystal form which is the most suitable for analysis and established the protocol for its preparation. Also, we analyzed the relationship between the absorbed X-ray dose of the crystal and the damage of the internal structure of the enzyme. The analysis enabled us to collect damage-less diffraction dataset. The best resolution of the obtained dataset was 0.99 angstrom.
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