| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
The interdomain interaction within the molecule of MyD88 was revealed by AFM observation and NMR spectroscopy. The interaction sites were also identified by mutagenesis. In addition, it was found by using NMR that the propeptide portion of the precursor of IL-18 extensively interacted with the main body of IL-18. CD spectra and NMR measurements revealed that the thermodynamic stability of the precursor was higher than that of mature IL-18. A peptide containing the propeptide sequence was found to bind to IL-18, reproducing the interaction seen in the precusor of IL-18. In addition, the crystal structure of IL-37 was determined.
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