| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Outline of Final Research Achievements |
Glycosylphosphatidylinositols (GPIs) are glycolipids that are attached to various proteins and act as their membrane anchors on the cell surface. GPIs also exist as free, non-protein linked glycolipids. The structure of core glycan part is conserved whereas side chains make variation. We aimed to clarify how GPI side chains are synthesized and to understand intracellular dynamics and pathological roles of free GPIs. We identified two enzymes that are involved in generation of a major side chain in mammalian GPI. Concerning free GPI, we found that GPIs synthesized in the endoplasmic reticulum of cells defective in GPI transamidase that transfers GPI to proteins, are transported to the cell surface. Using free GPI levels as a measure of GPI biosynthesis levels, we found a mechanism of GPI biosynthesis regulation. We also demonstrated that abnormally accumulated free GPIs cause autoinflammation.
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