| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
A single molecule FRET (Förster Resonance Energy Transfer) measurement of the two heads of dynein molecule directly indicated that a transition process between the stack structure and the separated structure. To explore the regulatory mechanism to induce an active motion state (separated structure) from an auto-inhibition state (stack structure), we investigated the structure and the function of dynactin molecule which is known as a principle regulatory protein of dynein. The results showed that dynactin has two antagonistic domains to promote or suppress dynein motility within a molecule, and dynactin has a flexible structure and multiple conformations, which suggests an auto-regulation mechanism within a dynactin molecule.
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