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Experimental and theoretical studies on function of single and multiple molecules

Research Project

Project/Area Number 16H04773
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

Higuchi Hideo  東京大学, 大学院理学系研究科(理学部), 教授 (90165093)

Co-Investigator(Kenkyū-buntansha) 茅 元司  東京大学, 大学院理学系研究科(理学部), 助教 (00422098)
佐々木 一夫  東北大学, 工学研究科, 教授 (50205837)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywords分子モーター / 1分子 / 少数分子 / キネシン / ミオシン / ステップ / 理論 / 細胞 / ダイニン / 運動 / 1分子計測 / モータータンパク質 / メカニズム / 生物物理
Outline of Final Research Achievements

Processive molecular motors, kinesin-1, myosin-V and dynein-1, that play a role in vesicle transport in cells take hundred steps without dissociation from microtubule and actin filament. We constructed the unified model that explains the dwell time of steps and the ratio of numbers of forward to backward steps from the simple rate of forward and backward steps. The model was fitted well to the data obtained previously and in this work.
The myosin assembly purified from skeletal muscle interacted with actin filament. The force and displacement were measured by optical tweezers. The energy calculated from the force and step displacement was larger than the energy liberated from one ATP molecule hydrolysis, suggesting that the step reaction is cooperative interaction of myosin molecules. The oscillatory motion of single cardiac cell was also explained by the cooperative interaction with reverse reaction.

Academic Significance and Societal Importance of the Research Achievements

これまでの生命科学は、多様性や複雑性に関する研究が多い一方で、物理学が得意とするような統一性や普遍性の研究が少なく、これらの分野の今後の発展が望まれている。その意味で、今回の運動を簡単な数式で統一的に説明した研究は、輸送タンパク質にとどまらず、ATPを利用する多くのタンパク質の運動を説明できる可能性を秘めており、学術的意義が大きい。また、心筋細胞の振動現象のメカニズム解明は、心臓の運動を記述する際重要である。従って、今後心臓や心臓疾患を理解するための基礎となり、学術的にも社会的にも意義がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (32 results)

All 2019 2018 2017 2016

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Open Access: 7 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 13 results,  Invited: 11 results) Book (2 results)

  • [Journal Article] 3D rotational motion of an endocytic vesicle on a complex microtubule network in a living cell2019

    • Author(s)
      1.Seohyun Lee and Hideo Higuchi
    • Journal Title

      bioRxiv

      Volume: 576702 Pages: 1-12

    • DOI

      10.1101/576702

    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Journal Article] A unified walking model for dimeric motor proteins2018

    • Author(s)
      K. Sasaki, M. Kaya, and H. Higuchi.
    • Journal Title

      Biophys. J.

      Volume: 115 Issue: 10 Pages: 1-12

    • DOI

      10.1016/j.bpj.2018.09.032

    • NAID

      130007648128

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Step Sizes and Rate Constants of Single-headed Cytoplasmic Dynein Measured with Optical Tweezers2018

    • Author(s)
      Yoshimi Kinoshita, Taketoshi Kambara, Kaori Nishikawa, Motoshi Kaya and Hideo Higuchi
    • Journal Title

      Sci. Rep

      Volume: 16333 Issue: 1 Pages: 1-13

    • DOI

      10.1038/s41598-018-34549-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Numerical method for vesicle movement analysis in a complex cytoskeleton network.2018

    • Author(s)
      Seohyun Lee, Hyuno Kim, Hideo Higuchi.
    • Journal Title

      Opt. Express

      Volume: 26 Issue: 13 Pages: 16236-16239

    • DOI

      10.1364/oe.26.016236

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ファイマンラチェット-生体分子の運動の理解にむけて-2018

    • Author(s)
      樋口秀男
    • Journal Title

      数理科学

      Volume: 56 Pages: 34-39

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Focus Stabilization by Axial Position Feedback in Biomedical Imaging Microscopy2018

    • Author(s)
      Seohyun Lee, Hyuno Kim, Hideo Higuchi
    • Journal Title

      IEEE Sensors Applications Symposium

      Volume: 2018 Pages: 309-314

    • DOI

      10.1109/sas.2018.8336767

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coordinated force generation of skeletal myosins in myofilaments through motor coupling.2017

    • Author(s)
      Motoshi Kaya*, Yoshiaki Tani, Takumi Washio, Toshiaki Hisada and Hideo Higuchi
    • Journal Title

      Nature Communications

      Volume: 16036 Issue: 1 Pages: 1-14

    • DOI

      10.1038/ncomms16036

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of spontaneous oscillations for a three state power stroke model2016

    • Author(s)
      Takumi Washio, Seine A. Shintani, Hideo Higuchi and Toshiaki Hisada
    • Journal Title

      Physical Review E

      Volume: 22 Issue: 2 Pages: 473-482

    • DOI

      10.1103/physreve.95.022411

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In vivo muscle force and muscle power during near-maximal frog jumps2016

    • Author(s)
      Eug Kuan Moo, Daniel R Peterson, Timothy R Leonard, Motoshi Kaya, Walter Herzog
    • Journal Title

      PLoS One

      Volume: 12 Issue: 3 Pages: 1-12

    • DOI

      10.1371/journal.pone.0173415

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quantitative evaluation of malignant gliomas damage induced by photoactivation of IR700 dye2016

    • Author(s)
      Morito Sakuma, Sayaka Kita and Hideo Higuchi
    • Journal Title

      Science and Technology of Advanced Materials

      Volume: 17 Issue: 1 Pages: 473-482

    • DOI

      10.1080/14686996.2016.1205936

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 3D Nanoscale Tracking Data Analysis for Intracellular Organelle Movement using Machine Learning Approach.2018

    • Author(s)
      Seohyun Lee, Hyuno Kim, Masatoshi Ishikawa, and Hideo Higuchi
    • Organizer
      International conference on Artificial Intelligence in Information and Communication
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of self-oscillatory contraction of cardiac muscle2018

    • Author(s)
      S. Shintani, T. Washio, Y. Hwang, M, Kaya and H. Higuchi
    • Organizer
      International symposium on Nanomedicine
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 荷領域の測定から明らかになったキネシン1分子のステップ運動2018

    • Author(s)
      近藤雄一, 佐々木一夫, 樋口秀男
    • Organizer
      生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 心機能に適した心筋ミオシンの集団的性質2018

    • Author(s)
      黄勇太, 樋口秀男, 茅元司
    • Organizer
      生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Three-dimensional vesicle motion in complex cytoskeletal network revealed by numerical analysis method2018

    • Author(s)
      Lee Seohyun , Hideo Higuchi
    • Organizer
      生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Step sizes and rate constants of single-headedcytoplasmic dynein2018

    • Author(s)
      木下慶美, 神原丈敏, 西川香里, 茅元司, 樋口秀男
    • Organizer
      生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Behavior of polymerized microtubules interacted with dyneins still attached on a doublet microtubule detected by laser tweezers2018

    • Author(s)
      藤原貴史, 真行寺千佳子, 樋口秀男
    • Organizer
      生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光ピンセット技術を用いた心筋ミオシン集団の力学的性質2018

    • Author(s)
      黄勇太, 樋口秀男, 茅元司,
    • Organizer
      ナノ学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 広い負荷領域におけるキネシン1 分子のステップ運動2018

    • Author(s)
      近藤雄一, 佐々木一夫, 樋口秀男
    • Organizer
      ナノ学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Trafficking of Endocytic Vesicles on Cytoskeleton in LiveCancer Cells. A3 Foresight 9th Meeting2017

    • Author(s)
      Seohyun Lee, Motoshi Kaya, Kohsuke Gonda, Hideo Higuchi.
    • Organizer
      A3 Foresight 9th Meeting
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Property of Cardiac Myosin Assemble Measured by Optical Trapping2017

    • Author(s)
      Yongtae Hwang, Hideo Higuchi.. Motoshi Kaya
    • Organizer
      A3 Foresight 9th Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Step sizes and rate constants of single-headed dynein measured by optical tweezers2017

    • Author(s)
      Yoshimi Kinoshita, Hideo Higuchi.
    • Organizer
      International Workshop Dynein 2017, International Conference Center, Awaji Yumebutai (2017.10.29~11.1)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanical activity of dynein and its dynamical regulation underlying oscillatory movement of sperm flagella2017

    • Author(s)
      Chikako Shingyoji, Hiroshi Yoke,Yasuhide Izawa, Izumi Nakano,Yuichi Inoue, Hideo Higuchi
    • Organizer
      International Workshop Dynein 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Damage of cancer cells evaluated by intensity fluctuation of images under phase contrast microscope2017

    • Author(s)
      Morihito. Sakuma, Yuichi. Kondo and Hideo Higuchi
    • Organizer
      11th International Symposium on Nanomedicine
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Step sizes and rate constants of single-headed dynein measured by optical tweezers2017

    • Author(s)
      Yoshimi Kinoshita, Hideo Higuchi.
    • Organizer
      International Workshop Dynein 2017,
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanical activity of dynein and its dynamical regulation underlying oscillatory movement of sperm flagella2017

    • Author(s)
      Chikako Shingyoji, Hiroshi Yoke,Yasuhide Izawa, Izumi Nakano,Yuichi Inoue, Hideo Higuchi.
    • Organizer
      International Workshop Dynein 2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Damage of cancer cells evaluated by intensity fluctuation of images under phase contrast microscope.2017

    • Author(s)
      Morihito. Sakuma, Yuichi. Kondo and Hideo. Higuchi
    • Organizer
      11th International Symposium on Nanomedicine
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The step size and microtubule-binding time of single-headed dynein.2017

    • Author(s)
      Yoshimi Kinoshita , Taketoshi Kambara, Kaori Nishikawa, Motoshi Kaya, Hideo Higuchi.
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan(2017.9.19~9.21)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cooperative force generation by muscle myosin molecules interacting with actin filaments.2016

    • Author(s)
      Hideo Higuchi, Motoshi Kaya, Takumi Washio, Seine Shintani.
    • Organizer
      KIAS Protein Science Soul Korea
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanism of synchronous force generations among skeletal myosins.2016

    • Author(s)
      Motoshi Kaya.
    • Organizer
      Symposium: Modeling and Manupilation of Life
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Handbook of dynein2018

    • Author(s)
      H. Higuchi and C. Shingyoji
    • Total Pages
      300
    • Publisher
      Pan Stanford Publishing
    • Related Report
      2017 Annual Research Report
  • [Book] 少数性生物学2016

    • Author(s)
      茅 元司
    • Total Pages
      178
    • Publisher
      日本評論社
    • ISBN
      4535788162
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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