| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
Vibrio alginolyticus has a single polar flagellum used for swimming motility. We found that FlhG, the negative regulator for flagellar number and ATPase, exists as a monomer in the presence of ATP and do not form dimer as seen for its paralog MinD. We suggest that an ATP-bound FlhG localizes at cell pole via the membrane protein HubP, and then directly inhibits FlhF, the positive regulator for flagellar number. On the other hand, FlhF intrinsically localizes at cell pole. We found that a GTP-bound FlhF forms dimer and its GTPase activity was stimulated by the N-terminal region of FlhG. Our current model proposes that the nucleotide-bound active states of FlhF and FlhG function at cell pole, and their regulatory activities to determine flagellar number is strictly balanced at pole, so as to generate only a single polar flagellum.
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