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Comprehensive research of signal transduction of cell

Research Project

Project/Area Number 16H04777
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

ISHIJIMA Akihiko  大阪大学, 生命機能研究科, 教授 (80301216)

Research Collaborator FUKUOKA Hajime  
CHE Yong-Suk  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywords走化性 / バクテリア / 生物物理
Outline of Final Research Achievements

To clarify the understanding of signal transduction of cell, we tried to measure multiple measuring systems. 1. using the mutant of CheZ that could not localize at cell pole, the delay time of switching depending the distance between the motor and cell pole was disappeared. 2. However, we tried to construct the fusion proteins that is function, it was still working state. 3. When the compulsive force was applied to the motor, a few molecules were more attached to the motor. Next stage is needed to consider theoretical proof.

Academic Significance and Societal Importance of the Research Achievements

従来の考えでは非常に小さな空間であるバクテリアは空間的な分布を考えずに一様として,化学反応,拡散現象で説明してきた.しかし,我々の研究により空間的な不均一性が発生していることを明らかにした.このことは細胞内の情報伝達を考える上で非常に重要な知見である.我々は国内・国際学会において発表,説明を行い,他の研究者らと議論を進めてきた.今後この空間の不均一性がどのように生理的に有利に働くかを明らかにしたい

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (18 results)

All 2019 2018 2017 Other

All Int'l Joint Research (1 results) Presentation (14 results) Book (3 results)

  • [Int'l Joint Research] University of Utah/Biology Department/John (Sandy) Parkinson(米国)

    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞内タンパク質動態に基づいた大腸菌走化性の1細胞解析2019

    • Author(s)
      福岡創,濱元樹,山越達矢,蔡栄淑,石島秋彦
    • Organizer
      2019年生体運動研究合同班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] FERT法による走化性受容体クラスター活性とべん毛モーター回転の1細胞同時計測2018

    • Author(s)
      福岡 創, 山越 達矢, 西村 彩莉奈, 蔡 栄淑, 石島 秋彦
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大腸菌におけるべん毛の回転方向とCheYの細胞内動態の同時計測2018

    • Author(s)
      山越 達矢, 蔡 栄淑, 石島 秋彦, 福岡 創
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大腸菌走化性応答におけるCheY極局在の役割2018

    • Author(s)
      蔡 栄淑, 石島 秋彦, 福岡 創
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] モーターの回転方向の同調的制御におけるCheR, CheBの役割2018

    • Author(s)
      濱元 樹, 蔡 栄淑, 石島 秋彦, 福岡 創
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Quantitative analysis for the ratio of WT and mutant receptors that collapses receptor cooperativity in chemotaxis in Escherichia coli2018

    • Author(s)
      小口 伸, 福岡 創, 石島 秋彦, 蔡 栄淑
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Difference on chemotaxis response of E.coli derived from the dependency of flagellar motor2018

    • Author(s)
      長瀧 瑛詔, 蔡 栄淑, 石島 秋彦, 福岡 創
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Quantitative observation of CheY-GFP binding to a flagellar motor in the presence of external load by electrorotation2018

    • Author(s)
      森島 健太, 蔡 栄淑, 石島 秋彦, 福岡 創
    • Organizer
      日本生物物理学会第56回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Minicell tethered assay that enables simultaneous observation of a flagellar motor rotation and the incorporation of stators to the motor2017

    • Author(s)
      Takao Nakajima, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      日本生物物理学会熊本年会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Development of simultaneous observation system for flagellar components and motor rotation with external load by electrorotation2017

    • Author(s)
      Kenta Morishima, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      日本生物物理学会熊本年会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Detection of the activity in receptor cluster by single cell FRET and motor rotation in Escherichia coli cell2017

    • Author(s)
      Yu Mitoro
    • Organizer
      日本生物物理学会熊本年会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Novel imaging method for chemotaxis protein using a super-duper chemiluminescent protein, Nano-lantern2017

    • Author(s)
      Shintaro Aso, Masahiro Nakano, Hajime Fukuoka, Takeharu Nagai, Akihiko Ishijima
    • Organizer
      日本生物物理学会熊本年会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Functional signaling-fluorescent fusion protein for the dynamics of signaling pathway in E.coli2017

    • Author(s)
      Ryota Shiono, Akihiko Ishijima, Hajime Hukuoka
    • Organizer
      日本生物物理学会熊本年会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Presentation] Computational simulation of spontaneous transition between active and inactive in whole chemoreceptor array in E. coli2017

    • Author(s)
      Tatsuki Hamamoto, Takashi Sagawa, Shin Koguchi, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      日本生物物理学会熊本年会
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Book] 生産と技術:「細胞内タンパク質動態と細胞応答の1細胞同時計測が明らかにする -大腸菌の情報伝達-」2018

    • Author(s)
      福岡創,蔡栄淑,石島秋彦
    • Total Pages
      60
    • Publisher
      社団法人 生産技術振興協会
    • Related Report
      2018 Annual Research Report
  • [Book] 少数性生物学ってなんだ?2017

    • Author(s)
      永井健治/企画
    • Total Pages
      137
    • Publisher
      羊土社
    • ISBN
      9784758125024
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Book] 光アライアンス 生命科学と光学顕微鏡-どのようにして照明してきたか2017

    • Author(s)
      福岡 創・蔡 栄淑・石島 秋彦
    • Total Pages
      62
    • Publisher
      日本工業出版
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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